Project description:The alkaliphilic halotolerant bacterium Bacillus sp. N16-5 often faces salt stress in its natural habitats. One-color microarrays was used to investigate transcriptome expression profiles of Bacillus sp. N16-5 adaptation reactions to prolonged grown at different salinities (0%, 2%, 8% and 15% NaCl) and the initial reaction to suddenly alter salinity from 0% to 8% NaCl. Salt induced gene expression was measured when culture was grown on different salinities (0%, 2%, 8% and 15% NaCl) to mid-logarithmic phase. And salt induced gene expression was also measured at 0 min, 10 min, 30 min, 60min, 120min after a sudden change salinity from 0% to 8% NaCl.

2015-01-03 | E-GEOD-64621 | biostudies-arrayexpress

Global Analysis of Carbohydrates Utilization of the Alkaline Hemicellulytic Bacteria Bacillus sp. N16-5

Project description:Alkaline hemicellulytic bacteria Bacillus sp. N16-5 has abroad substrate spectrum and exhibits great growth ability on complex carbohydrates. In order to get insight into its carbohydrate utilization mechanism, global transcriptional profiles were separately determined for growth on glucose, fructose, mannose, galactose, arabinose, xylose, galactomannan, xylan, pectin and carboxymethyl cellulose by using one-color microarrays.

2014-06-17 | GSE38276 | GEO

Differential regulation of genes in response to exogenous ganglioside GM2 treatment in Cancer cells.

Project description:Gangliosides have been implicated in various diseases including but not limited to cancer. Overexpression of one of these gangliosides, namely ganglioside GM2 has been associated with several cancers like Glioblastoma (GBM), Renal Cell Carcinoma (RCC) and several others. Despite the definite role of GM2 in tumor-induced host immune suppression has been extensively studied, not much was known regarding its involvement in the alteration of tumor cell behaviour. Our laboratory established that ganglioside GM2 plays a pivotal role in promoting migration and invasion of cancer cells and in inducing epithelial-mesenchymal transition (EMT) primarily by acquiring anoikis resistance and anchorage independence. Our initial data indicates that GM2's pro-tumorigenic function may be elaborated through modulation of a diverse, yet distinct signaling pathways. To address this, we undertook this task of mapping the differential regulation of genes in response to exogenous GM2 treatment in HeLa cells.

2025-12-31 | GSE296987 | GEO

Plasma Membrane Remodelling in GM2 Gangliosidoses Drives Synaptic Dysfunction

Project description:Glycosphingolipids (GSL) are important bioactive membrane components. GSLs containing sialic acids, known as gangliosides, are highly abundant in the brain and diseases of ganglioside metabolism cause severe early-onset neurodegeneration. The ganglioside GM2 is processed by β-hexosaminidaseA and when non-functional GM2 accumulates causing Tay-Sachs and Sandhoff diseases. We have developed i3Neuron-based disease models demonstrating storage of GM2 and severe endolysosomal dysfunction. Additionally, the plasma membrane (PM) is significantly altered in its lipid and protein composition. These changes are driven in part by lysosomal exocytosis causing inappropriate accumulation of lysosomal proteins on the cell surface. There are also significant changes in synaptic protein abundances with direct functional impact on neuronal activity. Lysosomal proteins are also enriched at the PM in GM1 gangliosidosis supporting that lysosomal exocytosis is a conserved mechanism of PM proteome change in these diseases. This work provides mechanistic insights into neuronal dysfunction in gangliosidoses highlighting that these are severe PM disorders with implications for other lysosomal and neurodegenerative diseases.

2025-06-24 | PXD064632 | Pride

Draconibacterium mangrovi strain:GM2-18

Project description:Draconibacterium mangrovi strain:GM2-18 Genome sequencing and assembly

| PRJNA600331 | ENA

Bacillus sp.

Project description:Bacillus sp. genome sequencing

| PRJNA672175 | ENA