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Bulk RNA seq of mouse epiblast stem cells with Mixl1 differential timing expression

ABSTRACT: Bulk RNA seq of mouse epiblast stem cells with Mixl1 differential timing expression

PROVIDER: PRJNA1175619 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31059965_1.fastq.gz	Fastqsanger.gz
	SRR31059965_2.fastq.gz	Fastqsanger.gz
	SRR31059966_1.fastq.gz	Fastqsanger.gz
	SRR31059966_2.fastq.gz	Fastqsanger.gz
	SRR31059967_1.fastq.gz	Fastqsanger.gz

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Bulk RNA seq of mouse epiblast stem cells with Mixl1 differential timing expression

Project description:During mouse gastrulation, the formation of germ layers proceeds concurrently with the transition pluripotency state and the allocation of the multipotent epiblast cells to the germ layer derivatives. Lineage analysis and fate-mapping studies have revealed that groups of cells in different regions of the epiblast and the primitive streak of the gastrulating embryo are allocated to separate progenitors of the ectoderm, mesoderm and endoderm lineages three germ layers with descendants of each progenitor contributing to specific types of germ layer derivatives. Spatiotemporal transcriptomic analysis of the epiblast cell population has inferred that besides the allocation of the epiblast cells to progenitors of germ layers, a population of putative mesendoderm progenitors may be present in the gastrulating embryos.

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Project description:Microarray analysis of isolated hES cells from day 3 of cardiac differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes. We identified 6,757 differentially regulated genes, of which 2,520 were upregulated â¥2-fold in the eGFP+ (MIXL1+) mesoderm population A stencil differentiation protocol was used to isolate mesodermal cells based on GFP expression from the MIXL1 locus. Microarray analysis of isolated cells from day 3 of differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes.

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