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MOF and Ce-MOF improve soil

ABSTRACT: MOF and Ce-MOF improve soil Raw sequence reads

PROVIDER: PRJNA1175978 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31072900_1.fastq.gz	Fastqsanger.gz
	SRR31072900_2.fastq.gz	Fastqsanger.gz
	SRR31072901_1.fastq.gz	Fastqsanger.gz
	SRR31072901_2.fastq.gz	Fastqsanger.gz
	SRR31072902_1.fastq.gz	Fastqsanger.gz

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MOF and Ce-MOF for FS soil

Project description:MOF and Ce-MOF for FS soil Raw sequence reads

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The histone acetyl-tranferase MOF is required for metabolic function in the liver .

Project description:Purpose: Conditional MOF deletion in mouse livers resulted in acute hepatic necrosis. The goal of this study was to identify transcriptomic changes in the livers of MOF-depeleted mice livers compared to WT. Methods: Transcriptomic profiles of MOF∆ and WT mouse livers were generated in triplicate. Sequenced reads were quality filtered using Trimmomatic. Quality reads were aligned to the mouse genome using TopHat2. Reads were counted using htseq-count and differentially expressed transcripts were identified using DESeq. Results: 1408 differentially expressed genes were identified between MOF∆ and WT livers. 774 genes were down-regulated in MOF∆ and primarilly associated with metabolic pathways. Branched chain amino acid and fatty acid metabolic pathways, which are deregulated in non-alcoholic fattly liver disease (NAFLD) prgression were down-regulated. 664 genes were upregulated and associated with increased proliferation. Conclusions: These data show that MOF is required for maintaining proper metabolic function in the liver and deregulation of MOF may be involved in progression of NAFLD.

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Expression data from Lean and Obese Rat Mammary Glands, with and without treatment with bazedoxifene + conjugated estrogens (BZA/CE)

Project description:Many risk-eligible women refuse tamoxifen for primary prevention of breast cancer due to concerns about common side effects such as vasomotor symptoms. Tamoxifen may also induce or worsen insulin resistance and hypertriglyceridemia, especially in women with obesity. Bazedoxifene/conjugated estrogens (BZA/CE) reduces vasomotor symptoms and is currently undergoing evaluation for breast cancer risk reduction. However, the impact of BZA/CE on insulin resistance and metabolic health, particularly in those with excess adiposity, is understudied. Here, we examined the effects of obesity on response to BZA/CE in a rat model of breast cancer risk using older ovary-intact rats. Female Wistar rats received carcinogen to increase mammary cancer risk and were fed a high-fat diet to promote obesity. Lean and obese rats were selected based on adiposity, then randomized to BZA/CE or vehicle for 8 weeks. BZA/CE reduced adiposity, enriched small (insulin-sensitive) mammary adipocytes, increased the abundance of beneficial metabolic gut microbes (Faecalbaculum rodentium and Odoribacter laneus), and reversed obesity-associated changes in lipids and adipokines. BZA/CE also reversed obesity-induced mammary enrichment of cell proliferation pathways, consistent with risk-reducing effects. Together, these data support the use of BZA/CE to improve metabolic health and reduce breast cancer risk in individuals with obesity. RNA from mammary glands was analyzed by gene expression microarray.

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Project description:We utilized primary MEFs to further delineate the role of MOF in proliferating cells and demonstrate that MOF directly activates genes required for cell cycle progression.

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