Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1177644 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR31131005_1.fastq.gz | Fastqsanger.gz | |||
| SRR31131005_2.fastq.gz | Fastqsanger.gz | |||
| SRR31131006_1.fastq.gz | Fastqsanger.gz | |||
| SRR31131006_2.fastq.gz | Fastqsanger.gz | |||
| SRR31131007_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 68 |
Similar Datasets
Project description:SMXL7 nuclear condensates regulate transcription and chromatin architecture in Arabidopsis
| PRJNA1182661 | ENA
Project description:This study investigates SMXL7's role in transcriptional repression and chromatin organization in Arabidopsis. SMXL7 forms nuclear condensates that sequester transcription factors and associate with H3K27me3 heterochromatin, thereby modulating chromatin accessibility and genome architecture. These findings uncover a link between hormone signaling and epigenetic regulation in plant development.
2025-06-30 | GSE281171 | GEO
Project description:This study investigates the role of SMXL7 protein condensates in regulating gene expression and chromatin organization in the model plant Arabidopsis thaliana. The researchers found that SMXL7 forms phase-separated nuclear condensates that sequester transcription factors and histone demethylases, thereby repressing gene expression. These condensates also associate with and modulate levels of the repressive histone mark H3K27me3, influencing higher-order chromatin structure. The findings reveal a novel mechanism linking plant hormone signaling to epigenetic regulation and nuclear architecture, with implications for understanding how plants control development in response to environmental cues. This work provides new insights into the functions of biomolecular condensates in transcriptional control and hormone signaling in plants.
2025-06-30 | GSE280943 | GEO
Project description:This study investigates the role of SMXL7 protein condensates in regulating gene expression and chromatin organization in the model plant Arabidopsis thaliana. The researchers found that SMXL7 forms phase-separated nuclear condensates that sequester transcription factors and histone demethylases, thereby repressing gene expression. These condensates also associate with and modulate levels of the repressive histone mark H3K27me3, influencing higher-order chromatin structure. The findings reveal a novel mechanism linking plant hormone signaling to epigenetic regulation and nuclear architecture, with implications for understanding how plants control development in response to environmental cues. This work provides new insights into the functions of biomolecular condensates in transcriptional control and hormone signaling in plants.
2025-06-30 | GSE280948 | GEO
Project description:This study investigates the role of SMXL7 protein condensates in regulating gene expression and chromatin organization in the model plant Arabidopsis thaliana. The researchers found that SMXL7 forms phase-separated nuclear condensates that sequester transcription factors and histone demethylases, thereby repressing gene expression. These condensates also associate with and modulate levels of the repressive histone mark H3K27me3, influencing higher-order chromatin structure. The findings reveal a novel mechanism linking plant hormone signaling to epigenetic regulation and nuclear architecture, with implications for understanding how plants control development in response to environmental cues. This work provides new insights into the functions of biomolecular condensates in transcriptional control and hormone signaling in plants.
2025-06-30 | GSE280945 | GEO
Project description:This study investigates the role of SMXL7 protein condensates in regulating gene expression and chromatin organization in the model plant Arabidopsis thaliana. The researchers found that SMXL7 forms phase-separated nuclear condensates that sequester transcription factors and histone demethylases, thereby repressing gene expression. These condensates also associate with and modulate levels of the repressive histone mark H3K27me3, influencing higher-order chromatin structure. The findings reveal a novel mechanism linking plant hormone signaling to epigenetic regulation and nuclear architecture, with implications for understanding how plants control development in response to environmental cues. This work provides new insights into the functions of biomolecular condensates in transcriptional control and hormone signaling in plants.
2025-06-30 | GSE280950 | GEO
Project description:This study investigates the role of SMXL7 protein condensates in regulating gene expression and chromatin organization in the model plant Arabidopsis thaliana. The researchers found that SMXL7 forms phase-separated nuclear condensates that sequester transcription factors and histone demethylases, thereby repressing gene expression. These condensates also associate with and modulate levels of the repressive histone mark H3K27me3, influencing higher-order chromatin structure. The findings reveal a novel mechanism linking plant hormone signaling to epigenetic regulation and nuclear architecture, with implications for understanding how plants control development in response to environmental cues. This work provides new insights into the functions of biomolecular condensates in transcriptional control and hormone signaling in plants.
2025-06-30 | GSE280949 | GEO
Project description:The genetic elements required to tune gene expression are partitioned in active and repressive nuclear condensates. Chromatin compartments include transcriptional clusters whose dynamic establishment and functioning depends on multivalent interactions occurring among transcription factors, cofactors and basal transcriptional machinery. However how chromatin players contribute to the assembly of transcriptional condensates has not been addressed. By interrogating the effect of KMT2D haploinsufficiency in Kabuki Syndrome, we found that MLL4 contributes in the assembly of transcriptional condensates through liquid-liquid phase separation. MLL4 loss-of-function impaired Polycomb-dependent chromatin compartmentalization, altering nuclear architecture. By releasing the nuclear mechanical stress through the inhibition of the mechano-sensor ATR, we re-established the mechano-signaling of mesenchymal stem cells and their commitment towards chondrocytes both in vitro and in vivo. This study supports the notion that in Kabuki Syndrome the haploinsufficiency of MLL4 causes an altered functional partitioning of chromatin, which determines the architecture and mechanical properties of the nucleus.
2020-09-22 | PXD021616 | Pride
Project description:Chromatin is partitioned into distinct topological domains in an activity-dependent manner, with topological boundaries limiting the interaction between adjacent domains. Recent studies support the concept that several well-established nuclear compartments are assembled as ribonucleoprotein condensates. Here we ask whether the physical processes driving the assembly of the nuclear condensates play any role in three-dimensional chromatin architecture. We report that the insulation of approximately 20% of topological boundaries in human embryonic stem cells is substantially weakened following brief treatment with 1,6-hexanediol, a chemical known to disrupt several nuclear condensates. The disrupted boundaries are characterized by a high level of transcription, striking spatial clustering, and the augmented presence of transcription units widely expressed in diverse cell types. These topological boundary regions tend to be spatially associated, even inter-chromosomally, and segregate with nuclear speckles. These observations reveal a previously unappreciated mode of genome organization mediated by conserved boundary elements harboring widely-expressed transcription units and associated transcriptional condensates.
2022-06-15 | GSE195561 | GEO
Project description:The genetic elements required to tune gene expression are partitioned in active and repressive nuclear condensates. These chromatin compartments include enhancer-associated clusters whose dynamic establishment and functioning depends on multivalent interactions occurring among transcription factors, cofactors and basal transcriptional machinery. However how chromatin modifiers contribute to the assembly of enhancer-associated condensates have not been addressed. We herein defined the non-catalytic function of MLL4 in driving the formation active nuclear condensates and its interplay with Polycomb compartment in determining the nuclear architecture. By interrogating the role of haploinsufficiency of KMT2D in Kabuki Syndrome, we found that MLL4 guides the assembly of enhancer-associated condensates through liquid-liquid phase separation. The loss-of-function of MLL4 impaired the correct chromatin compartmentalization of Polycomb proteins, altering nuclear architecture. By releasing the nuclear mechanical stress through the inhibition of the mechano-sensor ATR, we re-established the mechano-signaling of MSCs and their commitments towards chondrocytes. This study supports the notion that MLL4 contributes to the functional partitioning of chromatin, which is required to determine the structure and the mechanical properties of the nucleus.
2020-09-09 | GSE135550 | GEO