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ABSTRACT: RNA sequencing of Atractylodes chinensis

PROVIDER: PRJNA1177777 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31156760_1.fastq.gz	Fastqsanger.gz
	SRR31156760_2.fastq.gz	Fastqsanger.gz
	SRR31156761_1.fastq.gz	Fastqsanger.gz
	SRR31156761_2.fastq.gz	Fastqsanger.gz
	SRR31156762_1.fastq.gz	Fastqsanger.gz

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Transcriptomics coupled with Metabonomics for elucidating differences between the components in different varieties Atractylodes

Project description:Atractylodes macrocephala is a traditional Chinese medicine in China. It is widely used in clinic and the market demand is great. The quality difference between A. chinensis(Bunge) Koidz and A. japonica Koidz.ex Kitam is great, which seriously affects its clinical curative effect. Determination of Atractylosin by High-performance liquid chromatography. Transcriptomics coupled with Metabonomics for elucidating differences between the components in A. chinensis(Bunge) Koidz and A. japonica Koidz.ex Kitam. It was found that ACS, ACC, PAL and NOS were the key genes and metabolites related to the synthesis of atractylodes macrocephala. The decrease of salicylic acid content in A. chinensis(Bunge) Koidz may lead to the decrease of its ability to mediate the elicitor of endophytic fungi, resulting in the down-regulation of the expression of TGAL4 transcription factor and the up-regulation of DOGL3 transcription factor in A. chinensis(Bunge) Koidz cells, which may affect the accumulation of Atractylosin. The study above provides a theoretical basis for elucidating the biosynthesis pathway of atractylosin in A. chinensis(Bunge) Koidz.

2024-09-28 | GSE278290 | GEO

Project description:RNA-seq of Atractylodes chinensis

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Sex-specific small RNA profiling and target gene expression in dioecious and monoecious Pistacia chinensis

Project description:Pistacia chinensis Bunge is known as dioecious, but we have found wild monoecious individuals. In order to screen the candidate genes which may influence the sex expression or floral phenotypic differences of P. chinensis, the inflorescence buds for different sex types associated with the sex differentiation were selected and tested for small RNA sequencing. Sex-specific differentially expressed small RNA were discovered, combined with real-time PCR data, the regulation patterns of various sex types were first revealed. Our study represents the first detailed analysis of small RNA sequencing, providing more clues for understanding the mechanism of sex determination on P. chinensis.

2020-01-01 | GSE128023 | GEO

Atractylodes chinensis

Project description:Atractylodes chinensis Raw sequence reads

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Project description:Full-length transcriptome of Atractylodes chinensis

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RNA-seq of Taxus chinensis cells

Project description:We reported the application of high-throughput sequencing technology (RNA-seq) for the transcriptome of T. chinensis cells and the transcriptional alternatives of that responded to MeJA were comprehensively and quantitatively assessed with high-throughput sequencing technology (RNA-seq). By sequencing > 29 million reads (200 bp in length) of cDNA from each of MeJA-treated T. chinensis cells at 16 h (T16) and the control (T0), we identified 46,581 transcripts and uncovered 13,469 genes differentially expressed in response to MeJA. We provided functional clues for understanding the regulation mechanisms of MeJA-mediated defense responses and taxol biosynthesis.

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Project description:Purpose:We aimed to characterize genome-widely the miRNA population and phasiRNA-generating genes/loci in litchi (Litchi chinensis). Multiple high throughput sequencing strategies, including sRNA sequencing, parallel analysis of RNA ends (PARE) sequencing, and strand-specific RNA-seq, were applied in combination with thorough bioinformatics analyses using a variety of computational methods.

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Transcriptome Analysis Reveals the Biocontrol Mechanism of Endophytic Bacteria AM201, Rhodococcus sp., against Root Rot Disease of Atractylodes macrocephala

Project description:Transcriptome analysis was performed on the rhizome tissues of Atractylodes macrocephala under different treatments. The four treatments were: sterile water irrigation alone, FS root irrigation, FS and AM201 root irrigation, and FS combined with methyltobuzin (TM) root irrigation. And the differential genes between AM201 and FO groups were identified and compared, which helps to reveal the resistance mechanism of AM201 to Atractylodes macrocephala root rot disease

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Project description:Transcriptome data of Atractylodes chinensis after treatment with microbial inoculants

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Project description:This study aimed to identify the mode of action of Schisandra chinensis water extracts (SCW) and Schisandra chinensis ethanol extracts (SCE) in SW1783 cell line.

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