Project description:To understand the role of IFITM1 in HLECs, HLECs were transfected with siIFITM1 or sicontrol . RNAs isolated from these cells were subjected to RNA seq analysis. DOI: 10.1096/fj.202500783R
Project description:To understand the role of ID3 in human limbal epithelial cells (HLECs), HLECs were transfected with siID3 or sicontrol . RNAs isolated from these cells were subjected to RNA seq analysis
Project description:RNA profiling by RNA-Seq analyses showed that in comparison with the control Dotap-treated cells, miR-574-5p transfection for 24 h resulted in significant up-regulation of 277 genes and down-regulation of 80 genes by at least 2-fold in the miR-574-5p-transfected splenocytes. Gene ontology and pathway enrichment analyses showed that the 277 significantly up-regulated genes in miR-574-5p-transfected cells were highly enriched and clustered in pathways associated with interferon and cytokine signaling, antiviral mechanisms, translesion synthesis and autoimmunity .