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Pioneering new enhancers by GATA3: role of facilitating transcription factors and chromatin remodeling

ABSTRACT: Pioneering new enhancers by GATA3: role of facilitating transcription factors and chromatin remodeling

PROVIDER: PRJNA1180107 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR31173201_1.fastq.gz	Fastqsanger.gz
	SRR31173201_2.fastq.gz	Fastqsanger.gz
	SRR31173202_1.fastq.gz	Fastqsanger.gz
	SRR31173202_2.fastq.gz	Fastqsanger.gz

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Pioneering new enhancers by GATA3: role of facilitating transcription factors and chromatin remodeling [CUTnRUN]

Project description:The mechanistic details by which pioneer transcription factors interact with partner transcription factors and chromatin modification/remodeling enzymes to activate enhancers remains an emerging area of study. Here, we utilize inducible expression of the pioneer factor GATA3 and study cofactor association and structural alterations in local chromatin. ATAC-seq analysis and footprinting revealed co-occurrence of GATA3 with other transcription factors, including AP-1, in newly accessible chromatin. GATA3 and AP-1 co-localize at primed enhancers with p300 and BRG1 where nucleosome positioning is responsive to GATA3 location, not to AP-1. Genetic inhibition of AP-1 binding alters chromatin opening at some, but not most, GATA3-bound loci. Pharmacologic inhibition of SWI/SNF ATPase function results in loss of GATA3-dependent chromatin accessibility, binding, and alterations in local chromatin architecture. We conclude that GATA3-dependent gains in chromatin accessibility require chromatin remodeling and that accessibility at some loci is facilitated by a partner transcription factor, in this case AP-1.

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Pioneering new enhancers by GATA3: role of facilitating transcription factors and chromatin remodeling

Project description:This SuperSeries is composed of the SubSeries listed below.

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Interaction of the pioneer transcription factor GATA3 with nucleosomes

Project description:We developed a high-resolution nucleosome mapping method to explore nucleosome positioning around GATA3 binding sites during GATA3-mediated mesenchymal-to-epithelial transition. We found specific correlations between nucleosome positioning and pioneering activities of GATA3.

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