Project description:Barley (Hordeum vulgare L.) is amongst the oldest domesticated crop plants and remains one of the world's most important crop species. It is diploid with a haploid genome of 5.1 gigabases (Gb), twice the size of those of human and maize, and closely related to the most widely grown crop, hexaploid wheat. To meet global demand for food, feed and fibre, it is commonly agreed that reference genome sequences of our crop plants are urgently required to enable genome-assisted crop improvement. As part of the The International Barley Genome Sequencing Consortium (IBSC) we present here raw data obtained from Illumina (GAII) sequencing of RNA samples from nine different barley cultivars for the purpose of Single Nucleotide Polymorphism (SNP) discovery.
Project description:A transcriptome analysis of developing abi5 and wild type (R108) seeds from Medicago truncatula was performed to decipher the role of ABI54 in the regulation of late seed maturation and seed longevity.
Project description:Climate change has increased the frequency and intensity of floods that impact global agricultural productivity. To better understand the response mechanisms and evolutionary history of gene family member regulation across angiosperm phyla, we studied the rapid submergence response of rice, the legume Medicago truncatula, and two Solanum species, domesticated tomato (S. lycopersicum cv. M82) and its dryland-adapted wild relative S. pennellii. Response to hypoxic conditions was measured by analyzing transcriptional and post-translational regulation in root tips of each species. This was achieved by the use of Nuclei Tagged in specific Cell Types (INTACT) and Translating Ribosome Affinity Purification to obtain chromatin and sub-populations of gene transcripts. (1) Chromatin accessibility was evaluated by coupling INTACT with ATAC-seq (assay for Transposon-Accessible Chromatin). (2) INTACT was used to capture nuclear RNA (nRNA). (3) Polyadenylated mRNA (polyA RNA) was obtained by standard oligo(dT) selection. (4) Ribosome-associated polyA mRNA (polyA RNA) was obtained by use of Translating Ribosome Affinity Purification (TRAP). Ribosome footprinting (Ribo-seq) was accomplished by using TRAP to capture ribosome protected fragments after RNAseI digestion. Samples evaluated include the apical root tip (four species) and shoot region (Solanum species only) under control conditions and after 2 h of submergence
Project description:ABI4 (MtrunA17_Chr5g0437371) is an AP2 transcription factor that is upregulated during seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Tnt1 insertion mutants were identified and RNA sequencing was performed on two abi4 mutants and the wild type seeds at 13 DAP, 24 DAP and mature seeds.
Project description:HSFA9 (Medtr4g126070) is a seed-specific heat shock transcription factor that is upregulated during the later stages of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Tnt1 insertion mutants were identified and RNA sequencing was performed on mature seeds from two hsfa9 mutants and the wild type seeds.