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RNA sequencing of LT-HSCs from the bone marrow of unirradiated mice after treated with saline or carnosine

ABSTRACT: RNA sequencing of LT-HSCs from the bone marrow of unirradiated mice after treated with saline or carnosine

PROVIDER: PRJNA1181876 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31221145_1.fastq.gz	Fastqsanger.gz
	SRR31221145_2.fastq.gz	Fastqsanger.gz
	SRR31221146_1.fastq.gz	Fastqsanger.gz
	SRR31221146_2.fastq.gz	Fastqsanger.gz
	SRR31221147_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of LT-HSCs from the bone marrow of unirradiated mice after treated with saline or carnosine

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Project description:We have developed a new conditional transgenic mouse showing that MLL-ENL, at an endogenous-like expression level, induces leukemic transformation selectively in LT-HSCs. To investigate the molecular mechanism of leukemic transformation in LT-HSCs conditionally expressing MLL-ENL, we preliminarily performed comprehensive gene expression profiling of CreER-transduced LT-HSCs and ST-HSCs using cDNA microarray analysis. For initial screening of candidate genes invloved in the leukemic transformation, total RNA was extracted from colony-forming cells derived from LT-HSCs and ST-HSCs transduced with CreER or mock. Four samples were analyzed, and CreER-transduced LT/ST-HSC-derived cells were compared with mock-transduced LT/ST-HSC-derived cells, while CreER/mock-transduced LT-HSC-derived cells were compared with CreER/mock-transduced ST-HSC-derived cells.

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Project description:Here, we used bulk-RNAseq of bulk-HSCs (Lineage-c-Kit+Sca-1+CD150+CD34-/loFlk2- ), LT-HSCs (Hoxb5+Lineage-c-Kit+Sca-1+CD150+CD34-/loFlk2- ), and ST-HSCs (Hoxb5-Lineage-c-Kit+Sca-1+CD150+CD34-/loFlk2- ) from young (2-3 months) or aged (23-25 months) mice to compare age-associated transcriptomic changes.

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