Project description:The acute lymphoblastic leukemia cells lines JM-1, REH, Nalm-27, and SUP-B15 were analyzed for baseline expression of extacellular matrix and adhesion molecules using a pathway focused cDNA microarray (SABiosciences). RNA isolated from the ALL cell lines JM-1, REH, Nalm-27, and SUP-B15 was analyzed using the Extracellular Matrix and Adhesion Molecules Oligo GEArray (SABIosciences).
Project description:Accumulated research has suggested the importance of the adhesion molecules modulation as therapeutic approach for bronchial asthma. Adhesion molecules expression alteration contributes to the pathogenesis of asthma. In order to probe the relationship between expression imbalance of adhesion molecules and asthma pathogenesis, expression profiling of adhesion molecules was performed using cDNA microarray. The results showed that there were various adhesion molecules with abnormal expressions in peripheral blood leucocytes of asthma patients. RNA was extracted from leucocytes in peripheral blood of 4 normal adults and 6 asthma patients by using TRIzol Reagent. Microarray expression studies were performed using the GEArray Q Series Human Extracellular Matrix & Adhesion Molecules Gene Array (SABiosciences Corporation, USA). This microarray profiles the expression of 96 genes key to the functions of cell adhesion. A negative control (PUC18DNA and blank), and the housekeeping genes including β-actin, GAPDH, Cyclophilin A and ribose body protein L13a were spread on each chip.
Project description:The transcription factor ISL1 regulates a unique set of genes in retinal ganglion cells. KEGG Pathway analysis of differentially expressed genes in Isl1 cKO retina revealed multiple genes involved in axon guidance, extracellular matrix-receptor interactions, and cell adhesion molecules.
Project description:The transcription factor ISL1 regulates a unique set of genes in retinal ganglion cells. KEGG Pathway analysis of differentially expressed genes in Isl1 cKO retina revealed multiple genes involved in axon guidance, extracellular matrix-receptor interactions, and cell adhesion molecules.
Project description:SPARC is a matricellular glycoprotein involved in regulation of the extracellular matrix, growth factors, adhesion, and migration. SPARC-null mice have altered basement membranes and develop posterior sub-capsular cataracts with cell swelling and equatorial vacuoles. Exchange of fluid, nutrients, and waste products in the avascular lens is driven by a unique circulating ion current. Here we demonstrate that SPARC-null mouse lenses exhibit abnormal circulation of fluid, ion, and small molecules which leads to altered fluorescein distribution in vivo, loss of resting membrane polarization, and altered distribution of small molecules. Microarray analysis of SPARC-null lenses showed changes in gene expression of ion channels and receptors, matrix and adhesion genes, cytoskeleton, immune response genes, and cell signaling molecules. Our results demonstrate that the regulation of SPARC on cell-capsular matrix interactions can influence the circulation of fluid and ions in the lens, and the phenotype in the SPARC-null mouse lens is the result of multiple intersecting pathways. Experiment Overall Design: Lens epithelial cells from 7 lenses of littermate mice were isolated by laser capture microdissection. 3 wild-type lenses from 3 different mice and 4 knock-out lenses from 3 different mice were used as biological replicates.
Project description:While investigating the causes of unstable adhesiveness of primary human hepatocytes, we observed that non-adhering cells had some kind of extracellular matrix on their surface, and this extracellular matrix inhibited cell adhesion to culture vessels. Removal of the matrix from the cell surface of non-adhering cells improved cellular attachment to culture vessels. We also performed comparative gene expression analysis between adhering and non-adhering cells to elucidate the molecular basis of differential adhesion behavior. We investigated the unstable adhesiveness of primary human hepatocytes and found that non-adhering cells had extracellular matrix on their surface that inhibited cell adhesion. We also observed that matrix removal improved cellular attachment.