Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

ABSTRACT: Differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

PROVIDER: PRJNA1183361 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

Project description:Screening of differentially expressed miRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection. We independently transfected SW1990 and Panc-1 cells with Gli1 cDNA for mRNA and miRNA microarray analysis. The results unveiled significant differential expression patterns, with numerous miRNAs and mRNAs showing changes post-Gli1 transfection. Remarkably, miR-301a exhibited high expression in both SW1990 and Panc-1 cells transfected with Gli1 cDNA.

2025-01-29 | GSE281315 | GEO

Differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

Project description:Screening of differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection. We independently transfected SW1990 and Panc-1 cells with Gli1 cDNA for mRNA and miRNA microarray analysis. The results unveiled significant differential expression patterns, with numerous miRNAs and mRNAs showing changes post-Gli1 transfection. Remarkably, miR-301a exhibited high expression in both SW1990 and Panc-1 cells transfected with Gli1 cDNA

2025-01-29 | GSE281316 | GEO

Differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

Project description:Differentially expressed mRNAs in SW1990 and PANC-1 cells following GLI1 cDNA transfection.

| PRJNA1183369 | ENA

Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1

Project description:Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 3 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array GLI1 direct target genes were identified in Panc-1 cells. Gene expression profiles of Panc-1GLI1ER and Panc-1ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 3 hours after treatment of beta-estradiol (E2) at doses of 10nM.

2013-07-18 | E-GEOD-28901 | biostudies-arrayexpress

Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1

2013-07-18 | GSE28901 | GEO

Identification of GLI1 direct and indirect target genes in human pancreatic cancer cell line Panc-1

Project description:Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 24 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array

2017-04-01 | GSE28902 | GEO

Identification of ZIC2 target genes in human pancreatic cancer cell lines PANC-1 and KP-4

2014-04-14 | GSE39704 | GEO

Identification of ZIC2 target genes in human pancreatic cancer cell lines PANC-1 and KP-4

Project description:PANC-1Tet/ZIC2 and PANC-1Tet/empty were established from human pancreatic cancer cell line PANC-1. PANC-1Tet/ZIC2 cells express FLAG-tagged human ZIC2 on the withdrawal of DOX. On the other hand, PANC-1Tet/empty was transfected an empty vector for the control experiment. To identify ZIC2 target genes, total RNAs were purified from the cells before and 48 hours after the DOX withdrawal. Gene expression profiles were analyzed by AGILENT human 4x44k cDNA microarray. As well as ZIC2-inducible system, we performed ZIC2-knockdown experiments in PANC-1 human pancreatic cancer cells. After 96 hours transfection of siRNAs for ZIC2 and its control, total RNAs were purified and gene expression profiles were analyzed by AGILENT human 4x44k cDNA microarray. ZIC2 target genes were identified in PANC-1 cells. Gene expression profiles of PANC-1Tet/ZIC2 and PANC-1Tet/empty cells were analyzed by AGILENT human 4x44k cDNA microarray before and 48 hours after the DOX withdrawal. As well as ZIC2-inducible system, gene expression profiles of control- and ZIC2-knocdown PANC-1 cells were also analyzed by AGILENT human 4x44k cDNA microarray.

2014-04-14 | E-GEOD-39704 | biostudies-arrayexpress

GLI1 knock-down in Panc1 cells

Project description:Panc1 (human pancreatic adenocarcinoma cells) cells were transfected with control siRNA (targeting firefly luciferase, siLuc) or siRNA targeting GLI1 (siGLI1, Pool of four siRNAs). 72h following transfection, RNA was prepared for array analysis.

2012-03-31 | E-MEXP-3379 | biostudies-arrayexpress

The differences between CD166+ pancreatic cancer cells and CD166- cells

Project description:Microarray analyses using CD166+ and CD166- pancreatic cancer cell lines from both Panc-1 and SW1990 cells. We carried out microarray analyses using the CD166+ and CD166- separated cells from both Panc-1 and SW1990. The quality of the RNA samples was evaluated using a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). We used a Human HT-12v4 Expression BeadChip (Illumina, San Diego, CA) for these analyses. The data were analyzed using BeadStudio software version 3.2.3 (Illumina).

2014-02-25 | E-GEOD-55294 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data