Xylella fastidiosa
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA118559 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This is the study of the Heat Shock response of phytopathogenic bacteria Xylella fastidiosa. This series keeps the 25 minutes 40oC stimulus response (Aug 2005). Keywords: stress response; heat shock response
2006-06-01 | GSE3044 | GEO
Project description:In the xylem vessels of susceptible hosts, such as citrus trees or grapevines, Xylella fastidiosa forms biofilm like-colonies that can block water transport, which appears to correlate to disease symptoms. Besides helping host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMP). Here we show that gomesin, a potent AMP from a Brazilian tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min treatment with a sublethal concentration. Data from DNA microarray hybridizations revealed that among the up-regulated coding sequences (CDS), some are related to biofilm production. In addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by non-treated cells or cells exposed to streptomycin. We have also observed that treatment of X. fastidiosa with sublethal concentration of gomesin before inoculation in tobacco plants correlates with reduction in CVC symptoms, an effect possibly due to trapping of bacterial cells to fewer xylem vessels given the enhancement in biofilm production. Together, our results suggest that X. fastidiosa can selectively sense a sublethal concentration of gomesin modulating its gene expression to produce a stronger biofilm that may protect itself against the toxic effects of this AMP. Two-condition experiment, control (PW media) vs. gomesin-treated cells. Biological replicates: 5 control, 5 gomesin-treated, independently grown and harvested. Two replicates (left and right side of slide) per array. Total of 10 slides were hibridized, including dye-swap of and two self-self experiments.
2010-05-16 | E-GEOD-17605 | biostudies-arrayexpress
Project description:In the xylem vessels of susceptible hosts, such as citrus trees or grapevines, Xylella fastidiosa forms biofilm like-colonies that can block water transport, which appears to correlate to disease symptoms. Besides helping host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMP). Here we show that gomesin, a potent AMP from a Brazilian tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min treatment with a sublethal concentration. Data from DNA microarray hybridizations revealed that among the up-regulated coding sequences (CDS), some are related to biofilm production. In addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by non-treated cells or cells exposed to streptomycin. We have also observed that treatment of X. fastidiosa with sublethal concentration of gomesin before inoculation in tobacco plants correlates with reduction in CVC symptoms, an effect possibly due to trapping of bacterial cells to fewer xylem vessels given the enhancement in biofilm production. Together, our results suggest that X. fastidiosa can selectively sense a sublethal concentration of gomesin modulating its gene expression to produce a stronger biofilm that may protect itself against the toxic effects of this AMP.
2010-01-06 | GSE17605 | GEO
Project description:Xylella fastidiosa is a Gram-negative plant pathogen responsible for severe diseases in a variety of economically important crops. A critical aspect of its pathogenicity is the production of outer membrane vesicles (OMVs). While some knowledge exists on the molecular cargo of X. fastidiosa (Xf)-OMVs, nucleic acid cargoes remain unknown. Since OMVs protect DNA from degradation during transfer, vesiduction increases the efficiency of HGT, aiding bacterial evolution and adaptation. In order to understand if this mechanism is relevant for Xf, we sequenced DNA extracted from OMVs and compared it to DNA extracted from whole bacterial cells.
2025-05-30 | E-MTAB-14493 | biostudies-arrayexpress
Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa grown in minimal media XFM and XFM supplied with pectin or glucan (Host polysaccharides) , compared to cell grown in the complex media PWG. The cells grown in the minimal medium XFM supplied with host polysaccharides specially pectin are transmissible by the insect vector when delivered to the vector through artificial diet system. This does not happen with cells grown in the complex media. 4 (4 plex chips) study using total RNA recovered from 4 independents replicates for Xylella fastidiosa grown on PWG, XFM, XFM-glucan and XFM-pectin.
2009-10-03 | E-GEOD-18369 | biostudies-arrayexpress
Project description:Xylella fastidiosa Genome sequencing- Iran
| PRJNA915469 | ENA
Project description:Xylella fastidiosa is a Gram-negative plant pathogen responsible for severe diseases in a variety of economically important crops. A critical aspect of its pathogenicity is the production of outer membrane vesicles (OMVs). While some knowledge exists on the molecular cargo of X. fastidiosa (Xf)-OMVs, nucleic acid cargoes remain unknown. Other bacterial species show modulation of host immune signaling by OMV-packaged RNAs, aiding bacterial infection success. In order to understand if Xf employs a similar mechanism, we sequenced total RNA extracted from OMVs and compared it to RNA extracted from whole bacterial cells.
2025-05-30 | E-MTAB-14502 | biostudies-arrayexpress