Project description:The transcriptomes of FACS-sorted siglec-F+ alveolar macrophages and siglec-f- CD11b+ exudative macrophages from inducible airway GM-CSF over-expressing transgenic mice (DTGM) were compared to non-inducible littermate controls during influenza A virus infection.
2018-01-12 | GSE109099 | GEO
Project description:Infectome Analysis of Bat Kidneys in Yunnan
Project description:Purpose: Anti-angiogenic treatment is well established in the management of exudative AMD, but not sufficient in all patients. Characterization of factors driving this chronic disease could serve to identify additional treatment options. The purpose of this study was to assess gene expression patterns and distinct changes in cells derived from surgically extracted CNV (chorioidal neovascularization) membranes. Materials and Methods: Expression of >11.000 genes was analyzed by means of a microarray in cells cultured from two late-stage CNV membranes, compared to primary human RPE and ARPE-19 cells. A pathway analysis was performed to identify gene expression patterns associated with exudative AMD. Results: The analysis revealed significant alterations in gene sets associated with inflammatory processes in CNV-derived cells, involving up-regulation of pro-inflammatory factors IL6, C3, and C5 and downregulation of anti-inflammatory CFB and CFI. Factors associated with angiogenesis, such as VEGFA or ANGPT2, were not significantly regulated in the two RPE-derived cell lines. Conclusion: In late-stage CNV membrane-derived RPE, gene expression was shifted towards a pro-inflammatory state. Angiogenesis-associated factors were regulated differently in the two CNV-derived RPE membranes. While inflammation seems to be continuously stimulated by RPE associated with late exudative AMD, this seems not the case with regard to angioregulatory mechanisms.
