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Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia [snRNA-Seq]

ABSTRACT: Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia [snRNA-Seq]

PROVIDER: PRJNA1187215 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31370856.fastq.gz	Fastqsanger.gz
	SRR31370857.fastq.gz	Fastqsanger.gz
	SRR31370858.fastq.gz	Fastqsanger.gz
	SRR31370859.fastq.gz	Fastqsanger.gz
	SRR31370860.fastq.gz	Fastqsanger.gz

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Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia [snRNA-Seq]

Project description:Single-nucleus RNA sequencing of human periventricular white matter comparing healthy age-matched controls with vascular dementia patients.

2024-12-06 | GSE282111 | GEO

Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia [scRNA-Seq]

Project description:Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia [scRNA-Seq]

| PRJNA1187214 | ENA

snRNAseq of human vascular dementia white matter vs control

Project description:Transcriptional profiling of cells in white matter of the brain from cases with vascular dementia vs control cases

2022-09-28 | GSE213897 | GEO

snRNAseq of human vascular dementia white matter vs control

Project description:snRNAseq of human vascular dementia white matter vs control

| PRJNA882878 | ENA

Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia.

Project description:Vascular dementia (VaD) refers to a variety of dementias driven by cerebrovascular disease and is the second leading cause of dementia globally. VaD may be caused by ischemic strokes, intracerebral hemorrhage, and/or cerebral small vessel disease, commonly identified as white matter hyperintensities on MRI. The mechanisms underlying these white matter lesions in the periventricular brain are poorly understood. In this study we perform an extensive transcriptomic analysis on human postmortem periventricular white matter lesions in patients with VaD with the goal of identifying molecular pathways in the disease. We find increased cellular stress responses in astrocytes, oligodendrocytes, and oligodendrocyte precursor cells as well as transcriptional and translational repression in microglia in our dataset. We show that several genes identified by GWAS as being associated with white matter disease are differentially expressed in cells in VaD. Finally, we compare our dataset to an independent snRNAseq dataset of PVWM in VaD and a scRNAseq dataset on human iPSC-derived microglia exposed to oxygen glucose deprivation (OGD). We identify the increase of the heat shock protein response as a conserved feature of VaD across celltypes and show that this increase is not linked to OGD exposure. Overall, our study is the first to show that increased heat shock protein responses are a common feature of lesioned PVWM in VaD and may represent a potential therapeutic target.

| S-EPMC11661092 | biostudies-literature

Single-nucleus RNA sequencing of human periventricular white matter in vascular dementia [scRNA-Seq]

Project description:Single-cell RNA sequencing of human iPSC-derived microglia (iMGL) exposed to normoxia, hypoxia or oxygen-glucose deprivation (OGD) for 24 hrs.

2024-12-06 | GSE282112 | GEO

Circular RNA expression alteration in peripheral blood of premature infants with periventricular white matter damage

Project description:Periventricular white matter damage (PWMD) is the principal pathological type of brain damage in premature. It causes irreversible damage to the overall function of the central nervous system resulting in cerebral palsy, convulsions, epilepsy, cognitive, motor dysfunction and other late effects. CircRNAs are participate in the biological processes underlying many nervous system diseases. However, the circRNA expression profile of peripheral venous blood of premature infants with PWMD is not completely understood. Three premature with white matter damage (PWMD group) and three infants without brain injury (Normal group) were enrolled. Peripheral venous blood was collected from both groups for extraction of RNA and circRNA sequencing was performed. The RNA-seq technique was used to screen the differentially expressed circRNA in peripheral blood of infants with PWMD. The accuracy of sequencing results was verified by quantitative reverse transcription polymerase chain reaction (q-PCR) to the differentially express partial circRNA in the sequencing results. Bioinformatics analysis of Host genes was performed with differential circRNA. TargetScan and Miranda were used to predict circRNA-binding miRNAs and mapped into a circRNA-miRNA co-expression network. There were 119 significantly different circRNAs as compared with premature without brain injury, along with 1 circRNA was up-regulated and 4 circRNAs were down-regulated expression in the PWMD group. Combined with the existing research results and bioinformatics analysis results after sequencing, it is suggested that circRNA may regulate the occurrence and development of white matter damage in premature infants by interacting with miRNA. This first study of its kind further identified the expression profile of circRNA in peripheral blood of premature with WMD, and provide a novel targets for further investigation about the molecular mechanisms underlying PWMD and potential therapeutic intervention.

2020-05-20 | GSE131475 | GEO

Gene expression changes in the periventricular white matter of neonatal piglets at a defined 48h time point following a hypoxic-ischemic insult: a comparison of piglets left untreated and those treated with a remote post-conditioning stimulus applied to the hind leg immediately after the insult.

Project description:Remote ischemic postconditioning (RIPostC) is a promising therapeutic intervention whereby brief episodes of ischemia/reperfusion of one organ (limb) mitigate damage in another organ (brain) that has experienced severe hypoxia-ischemia. 16 Large White newborn female piglets were exposed to a tightly controlled hypoxic-ischemic insult using magnetic resonance spectroscopy (MRS) biomarkers. After hypoxia-ischemia (HI), piglets were randomized to: (i) no intervention (nâ=â8); (ii) RIPostC â with four, 10-min cycles of bilateral lower limb ischemia/reperfusion immediately after HI (nâ=â8). Piglets in the remote post-conditioned group exhibited a reduction in white matter proton MRS lactate/N acetyl aspartate and increased whole brain phosphorus-31 MRS ATP over the 48âh after hypoxia-ischemia. Cell death (apoptosis) was reduced with RIPostC in the periventricular white matter, internal capsule and corpus callosum. There was also reduced microglial activation in corpus callosum and more surviving oligodendrocytes in corpus callosum and periventricular white matter. Our results demonstrate changes in the expression of 74 genes in the periventricular white matter 63 were down regulated and 11 were upregulated, that accompany the reductions in cell death and microglial activation seen following a remote post conditioning stimulus at a single 48h time point. (significance was calculated at p<0.05, one way ANOVA, Mann-Whitney unpaired, noe were found to be significant following a Benjamini-Hochberg FDR multipe testing correction). Many of these genes are thought to be important for the mediation of the neuroprotective effects of post-conditioning which suggests its genomic effects may persist for 48h. A full description of this work can be accessed at doi: 10.1177/0271678X15608862.

2016-09-21 | E-MTAB-5125 | biostudies-arrayexpress

Deconstructing the Intercellular Interactome in Vascular Dementia with Focal Ischemia for Therapeutic Applications

Project description:Vascular dementia (VaD), the second-leading cause of dementia, is primarily a white matter ischemic disease with no direct therapies. Cell-cell interactions within lesion sites dictate disease progression or repair. To elucidate key intercellular pathways, we employed a VaD mouse model with focal ischemia replicating many elements of the complex pathophysiology of human VaD, combined with transcriptomic and functional analyses. Integrating cell-type-specific mouse VaD transcriptomes and human VaD snRNA-Seq data, plus a custom ligand-receptor database (4,053 human and 2,032 mouse pairs), conserved dysregulated intercellular pathways in both species were identified. We demonstrate that two intercellular signaling systems, Serpine2-Lrp1 and CD39-A3AR, are disrupted in VaD. Reduced Serpine2 expression enhances oligodendrocyte progenitor cell (OPC) differentiation, promoting repair, while an A3AR-specific agonist—currently in clinical trials for psoriasis—restores tissue integrity and behavioral function in the VaD model. This study reveals intercellular signaling targets and provides a foundation for developing innovative therapies for VaD.

2025-07-07 | GSE298799 | GEO

Transcriptomic analysis of age-associated periventricular lesions reveals dysregulation of the immune response

Project description:Age-associated Periventricular white matter lesions (PVL), reflecting white matter injury, are a common feature of the ageing brain. The aim of this study was to evaluate the transcriptomic profile of PVL using microarray analysis, in order to identify which pathways and/or gene expression changes may contribute to the pathogenesis of these lesions.

2020-11-18 | GSE157363 | GEO

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