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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-mES]

ABSTRACT: SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-mES]

PROVIDER: PRJNA1187535 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31379732_1.fastq.gz	Fastqsanger.gz
	SRR31379732_2.fastq.gz	Fastqsanger.gz
	SRR31379733_1.fastq.gz	Fastqsanger.gz
	SRR31379733_2.fastq.gz	Fastqsanger.gz
	SRR31379734_1.fastq.gz	Fastqsanger.gz

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-mES]

Project description:SPT6 (suppressor of Ty 6) is a conserved histone chaperone that facilitates transcription elongation, nucleosome reassembly, and RNA polymerase II progression. The functions of SPT6 in regulating chromatin remain not fully understood. Here we show that SPT6 localized to H3K14Ac enriched regions and promoted H3K14Ac densities in mouse embryonic stem cells (mESCs). Loss of SPT6 results in decreased H3K14Ac and transcription. Mechanistically, we showed that SPT6 enhances the chromatin binding levels of acetyltransferases KAT7 and KAT6A/KAT6B. In addition, we showed that SPT6 also promotes H3K14Ac in a KAT7/6A/6B independent manner. Our findings identify a vital role of SPT6 in maintaining the integrity of H3K14Ac profile in mESCs, thus suggesting a crosstalk between transcription machinery and H3K14Ac in mammals.

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [PRO-seq]

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [RNA-seq]

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [PRO-seq]

Project description:SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [PRO-seq]

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [RNA-seq]

Project description:SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [RNA-seq]

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-HEK293T]

Project description:SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-HEK293T]

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Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction

Project description:Spt6 is a histone chaperone that associates with RNA polymerase II and deposits nucleosomes in the wake of transcription. Although Spt6 has an essential function in nucleosome deposition, it is not known whether this function is regulated by post-translational modification. Here, we report that casein kinase II (CKII) phosphorylation of Spt6 directs nucleosome reassembly at the 5’ ends of a broad range of genes to prevent aberrant antisense transcription and enforce transcriptional directionality. Mechanistically, we show that interaction of Spt6 with Spn1 – a constitutive binding partner required for chromatin reassembly and full recruitment of Spt6 to genes is positively regulated by CKII phosphorylation of Spt6. Together, our study defines a previously unknown function for CKII phosphorylation in transcription, and further, highlights the importance of post-translational modification as a mechanism to fine-tune the functions of histone chaperones.

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Histone H3K9 and H3K14 acetylation mark active enhancers and promoters together with bivalent promoters in mouse embryonic stem cells

Project description:Transcription regulation in pluripotent embryonic stem (ES) cells is a complex process that involves multitude of regulatory layers, one of which is post-translational modification of histones. Here we have investigated the genome-wide occurrence of two histone marks, acetylation of histone H3K9 and K14 (H3K9ac and H3K14ac), in mouse ES cells. We demonstrate genome-wide that H3K9ac and H3K14ac show very high correlation. Examination of H3K9ac and H3K14ac in mES cells

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