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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [PRO-seq]

ABSTRACT: SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [PRO-seq]

PROVIDER: PRJNA1187537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31381464_1.fastq.gz	Fastqsanger.gz
	SRR31381464_2.fastq.gz	Fastqsanger.gz
	SRR31381465_1.fastq.gz	Fastqsanger.gz
	SRR31381465_2.fastq.gz	Fastqsanger.gz
	SRR31381466_1.fastq.gz	Fastqsanger.gz

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SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [PRO-seq]

Project description:SPT6 (suppressor of Ty 6) is a conserved histone chaperone that facilitates transcription elongation, nucleosome reassembly, and RNA polymerase II progression. The functions of SPT6 in regulating chromatin remain not fully understood. Here we show that SPT6 localized to H3K14Ac enriched regions and promoted H3K14Ac densities in mouse embryonic stem cells (mESCs). Loss of SPT6 results in decreased H3K14Ac and transcription. Mechanistically, we showed that SPT6 enhances the chromatin binding levels of acetyltransferases KAT7 and KAT6A/KAT6B. In addition, we showed that SPT6 also promotes H3K14Ac in a KAT7/6A/6B independent manner. Our findings identify a vital role of SPT6 in maintaining the integrity of H3K14Ac profile in mESCs, thus suggesting a crosstalk between transcription machinery and H3K14Ac in mammals.

2025-05-22 | GSE282181 | GEO

SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [RNA-seq]

2025-05-22 | GSE282180 | GEO

SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-HEK293T]

2025-05-22 | GSE282179 | GEO

SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-mES]

2025-05-22 | GSE282178 | GEO

SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [RNA-seq]

Project description:SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [RNA-seq]

| PRJNA1187539 | ENA

SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-mES]

Project description:SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-mES]

| PRJNA1187535 | ENA

SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-HEK293T]

Project description:SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-HEK293T]

| PRJNA1187536 | ENA

Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction

Project description:Spt6 is a histone chaperone that associates with RNA polymerase II and deposits nucleosomes in the wake of transcription. Although Spt6 has an essential function in nucleosome deposition, it is not known whether this function is regulated by post-translational modification. Here, we report that casein kinase II (CKII) phosphorylation of Spt6 directs nucleosome reassembly at the 5’ ends of a broad range of genes to prevent aberrant antisense transcription and enforce transcriptional directionality. Mechanistically, we show that interaction of Spt6 with Spn1 – a constitutive binding partner required for chromatin reassembly and full recruitment of Spt6 to genes is positively regulated by CKII phosphorylation of Spt6. Together, our study defines a previously unknown function for CKII phosphorylation in transcription, and further, highlights the importance of post-translational modification as a mechanism to fine-tune the functions of histone chaperones.

2018-11-30 | GSE122620 | GEO

SPT6 functions in transcriptional pause-release via PAF1C recruitment [PRO-seq]

Project description:In vitro studies identified various factors including P-TEFb, SEC, SPT6, PAF1, DSIF, and NELF functioning at different stages of transcription elongation driven by RNA polymerase II (RNA Pol II). What remains unclear is how these factors cooperatively regulate pause/release and productive elongation in the context of living cells. Using an acute protein-depletion approach, we report that SPT6 depletion results in release of paused RNA Pol II. Short genes demonstrate a prominent release and a subsequent increase in mature transcripts, whereas long genes fail to yield mature transcripts due to a loss of processivity. Unexpectedly, the recruitment of PAF1 complex (PAF1C) to RNA Pol II fails upon SPT6 depletion, leading to the release of NELF-bound RNA Pol II into the gene bodies. Furthermore, SPT6 depletion impairs heat shock-induced pausing, pointing to a role for SPT6 in regulating RNA Pol II pause-release through PAF1C recruitment and NELF removal during the early elongation.

2022-08-09 | GSE202187 | GEO

Establishment of constitutive heterochromatin in Drosophila early embryos involves H3K14ac-mediated recruitment of Eggless/SetDB1

Project description:The establishment of heterochromatin is a crucial process in the early embryonic development. Recent research suggests that SetDB1 is necessary for heterochromatin establishment in the early embryonic development of Drosophila. Additionally, SetDB1 has been found to bind to H3K14ac in mammalian cells. However, the role of the H3K14ac in heterochromatin establishment during embryogenesis remains unknown. Here we discovered that the active modification H3K14ac serves as an intergenerational marker from oocytes to early embryos, and it is recognized by Eggless/SetDB1 through its Tudor domains. Furthermore, we have introduced a point-mutation in the endogenous Eggless/SetDB1 locus, resulting in the loss of H3K14ac-binding. Both H3K14ac attenuation and SetDB1 mutant embryos exhibit abnormal heterochromatin formation and genomic instability. We reveal a novel model of heterochromatin establishment via H3K14ac-mediated recruitment of SetDB1.

2024-08-06 | GSE242239 | GEO

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