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Transcriptional changes in PC12 cells induced by repetitive transcranial magnetic stimulation (rTMS) in MSCs-inhibited inflammatory conditions

ABSTRACT: Transcriptional changes in PC12 cells induced by repetitive transcranial magnetic stimulation (rTMS) in MSCs-inhibited inflammatory conditions

PROVIDER: PRJNA1188501 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31407980_1.fastq.gz	Fastqsanger.gz
	SRR31407980_2.fastq.gz	Fastqsanger.gz
	SRR31407981_1.fastq.gz	Fastqsanger.gz
	SRR31407981_2.fastq.gz	Fastqsanger.gz
	SRR31407982_1.fastq.gz	Fastqsanger.gz

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Transcriptional changes in PC12 cells induced by repetitive transcranial magnetic stimulation (rTMS) in MSCs-inhibited inflammatory conditions

Project description:Neuronal death is the primary cause of poor outcomes in cerebral ischemia. The Inflammatory infiltration in the early phase of ischemic stroke plays a vital role in triggering neuronal death. Either transplantation of mesenchymal stem cells (MSCs) derived from humans or repetitive transcranial magnetic stimulation (rTMS) have respectively proved to be neuroprotective and anti-inflammatory in cerebral ischemia. However, either treatment above has its limitations. Whether these two therapies have synergistic effects on improving neurological function and the underlying mechanisms remains unclear.Using an in vitro model of PC12 nerve cell co-culture with MSCs, we demonstrated a transcriptional response pattern, including REST, in which rTMS inhibited PANoptosis of PC12 cells in a low-inflammation environment induced by MSCs. This molecular investigation is helpful in providing a basis for sorting out the complex molecular mechanism of combination of rTMS and MSCs in treating ischemic stroke.

2024-12-06 | GSE282396 | GEO

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VIVO

Project description:Transcranial Magnetic Stimulation (TMS) is a noninvasive technique that uses pulsed magnetic fields to affect the physiology of the brain and central nervous system. Repetitive TMS (rTMS) has been used in the study and treatment of neurological conditions including major depression, stroke, epilepsy, schizophrenia, multiple sclerosis, Parkinson’s, and Alzheimer’s disease, as well as other, non-neurological disorders. Among the effects of rTMS, low-frequency protocols (≤ 1Hz) are generally understood to result in cortical inhibition, whereas high-frequency protocols (≥ 3Hz) increase cortical excitability. However, the complex molecular basis of rTMS is largely unexplored. Using three complementary rat models, in vitro, ex vivo, and in vivo, we show complex patterns of hippocampal and neocortical transcriptional response to stimulation in glutamatergic and GABAergic signaling pathways, as well as multiple inflammatory pathways, among others. This broad-based molecular survey helps provide a foundation to tease out the complex molecular mechanisms of the effects of rTMS.

2023-05-30 | GSE230149 | GEO

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_EX_VIVO

2023-05-30 | GSE230148 | GEO

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VITRO

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High-Frequency Repetitive Transcranial Magnetic Stimulation (rTMS) Protects Against Ischemic Stroke by Inhibiting M1 Microglia Polarization through Let-7b-5p/HMGA2/NF-κB Signaling Pathway

Project description:Modulating M1/M2 polarization is a potential therapy for treating ischemic stroke. Repetitive transcranial magnetic stimulation (rTMS) held the capacity to regulate astrocytic polarization, but little is known about rTMS effects on microglia. Therefore, the present study aimed to investigate whether and how rTMS influence microglia polarization in ischemic stroke models. The 10-Hz rTMS was applied to transient middle cerebral artery occlusion (MCAO) rats and oxygen and glucose deprivation/ reoxygenation injured BV2 cells. Western blot, immunofluorescence and ELISA were used to detect M1/M2 markers. High-throughput sequencing, RT-PCR and FISH staining were adopted to test microRNA changes. The 10-Hz rTMS inhibited ischemia/reperfusion induced M1 microglia and significantly increased let-7b-5p. HMGA2 was proved to be the target protein of let-7b-5p and its downstream NF-κB signaling pathway were inhibited by rTMS. Microglia culture medium (MCM) collected from rTMS treated microglia had low TNF-α but high IL-10 concentration, leading to reduced neural death, minor ischemic volumes and improved functional recovery of MCAO animals. However, knockdown of let-7b-5p by antagomir reversed rTMS effects on microglia. In conclusion, high-frequency rTMS could improve functional recovery through inhibiting M1 microglia polarization via regulating let-7b-5p/HMGA2/NF-κB signaling pathway in cerebral ischemic stroke models.

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Repetitive transcranial magnetic stimulation induces cortical layer, brain region, and protocol dependent neural plasticity [Spatial transcriptomics]

Project description:Repetitive transcranial magentic stimulation (rTMS) is a non-invasive toll commonly used to study neural plasticity processes and treat neurological disorders. Despite the popularity of rTMS, it is still unclear what neural plasticity mechanisms are induced in the brain regions at and beyond the stimulation site, and how this varies with different rTMS protocols. Here we used spatial transcriptomics to map the neural plasticity mechanisms induced across cortical and sub-cortical regions following intermittent or continuous theta-burst stimulation protocols to the mouse sensorimotor cortex. Our results revealed that rTMS alters the expression of genes related to several cellular processes and neural plasticity mechanisms across the brain which was both brain region and rTMS protocol dependent. In the cortex, the effect of rTMS was not only dependent on the cortical region, but also the cortical layer within each cortical region. These findings uncover the neural plasticity mechanisms induced across the brain following rTMS and help inform how different stimulation protocols can be used to drive specific neural plasticity mechanisms.

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Repetitive transcranial magnetic stimulation induces cortical layer, brain region, and protocol dependent neural plasticity [Bulk RNA-seq]

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Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS).

Project description:Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS).

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Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VITRO

Project description:Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VITRO

| PRJNA957741 | ENA

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VIVO

Project description:Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VIVO

| PRJNA957742 | ENA