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Analysis of transcriptome and metabolome characteristics of blood in yaks at different reproductive stages

ABSTRACT: Analysis of transcriptome and metabolome characteristics of blood in yaks at different reproductive stages

PROVIDER: PRJNA1188607 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31416700_1.fastq.gz	Fastqsanger.gz
	SRR31416700_2.fastq.gz	Fastqsanger.gz
	SRR31416701_1.fastq.gz	Fastqsanger.gz
	SRR31416701_2.fastq.gz	Fastqsanger.gz
	SRR31416702_1.fastq.gz	Fastqsanger.gz

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Analysis of transcriptome and metabolome characteristics of blood in yaks at different reproductive stages

Project description:The reproductive physiology of yaks differs significantly from that of other cattle breeds due to late sexual maturity, low fecundity and short oestrus time. How to improve the reproductive efficiency of yaks has become the main research content and goal of yak reproduction technology. In this study, we collected blood samples from adult female yaks (4-8 years old) during different reproductive periods, including the period of anestrus (Y-A), estrus (Y-E) and pregnancy (Y-P), and investigated the changes of RNA expression and steroid hormone levels in yaks during different reproductive periods by using RNA-seq and target metabolomics, and screened for the genes and regulatory pathways that were differentially expressed. and related regulatory pathways. DEGs such as PDK4, ALAS2, GLP1R, SLC25A39, PGAP6, FOS, CD36, MMP9 and BCL-6 were identified to play key roles in ovarian function, follicular development, hormone homeostasis and energy metabolism. Functional annotation and enrichment analysis indicated that DEGs were involved in ovarian angiogenesis, hormone synthesis and follicular development. It was found that SLC25A39 may affect glucocorticoid homeostasis and physiological readiness by regulating energy metabolism during anestrus, MARCHF2 and DHEA may be closely related to reproductive hormone fluctuation and system activation during estrus, glucocorticoid down-regulation in pregnancy and maintenance of hormone homeostasis and regulation of immune tolerance by DHEA. The results of this study provide a theoretical basis for improving the reproductive performance of yaks and further analysing the reproductive characteristics of yaks.

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Ribo-seq analysis elucidates the dynamic translation landscape of yak ovarian tissue across different reproductive stages

Project description:Translation, as a crucial link in the transmission of genetic information, plays an indispensable regulatory role in follicular development, ovulation, corpus luteum formation, and degeneration. In order to deeply analyze the molecular mechanisms of reproductive regulation at the translation level, our study focused on the ovarian tissues of yaks at different reproductive stages to analyze gene expression changes using Ribosome profiling and integrating RNA-seq data. Meanwhile, the small open reading frames (sORFs) of ovarian tissue was characterized, and the impact of the translation efficiency of focused genes on their sequence feature was analyzed. The results showed that over 80% of genes in the two groups exhibited inconsistent changes at the transcription and translation levels, indicated that the changes in genes at both levels were complex and not simply synergistic. Upon the pathway enrichment analysis, the signaling pathways involved in these differentially expressed genes were further clarified, such as the estrogen signaling pathway PI3K-Akt, MAPK, calcium signaling pathway and ovarian steroidogenesis. Further research has found that some genes related to ovarian function have inconsistent expression at two levels and exhibit dynamic changes in translation activity, including PALB2, EGR2, BMP7, SOX9, PIK3R2, and WNT2B. Besides, we identified 66 predicted translatable small open reading frames (sORFs) and also explored the impact of upstream ORFs (uORFs) on the translation efficiency of downstream major ORFs (mORFs). This study systematically revealed the gene expression characteristics of ovarian tissues in yaks at different reproductive stages from the perspective of translational level for the first time, providing a new perspective for a deeper understanding of the physiological mechanisms of yak reproduction.

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