Project description:To study the population genetics context of the Saqqaq individual we carried out Illumina Bead-Array-based genotyping on four native North American and twelve north Asian populations.
Project description:Array CGH analysis of Helicobacter pylori strains isolated from a North American cohort of symptomatic pediatric patients. Keywords: genotyping_design
Project description:Legumes have evolved lineage-specific peptides to control rhizobia and sustain nitrogen fixation. In inverted repeat-lacking clade (IRLC) legumes, nodule-specific cysteine-rich (NCR) peptides enforce terminal bacteroid differentiation, maximizing symbiotic output but restricting nodule lifespan. By contrast, Robinioid legumes, the IRLC’s sister clade, lack NCRs yet yet include perennial species that maintain productive nodules for decades. Here we identify a nodule-proline-glycine-rich peptide family (NPGs) as a conserved, genus-wide innovation of Robinia. NPGs are highly abundant, localize to the symbiosome membrane, and are intrinsically disordered. Exposure of Mesorhizobium robiniae to recombinant NPGs reprograms physiology, inducing nitrogenase expression while dampening growth without loss of viability. NPGs thus exemplify a distinct, reversible strategy of symbiont control, expanding the peptide repertoire that supports nitrogen fixation in perennial legumes.
Project description:Legumes have evolved lineage-specific peptides to control rhizobia and sustain nitrogen fixation. In inverted repeat-lacking clade (IRLC) legumes, nodule-specific cysteine-rich (NCR) peptides enforce terminal bacteroid differentiation, maximizing symbiotic output but restricting nodule lifespan. By contrast, Robinioid legumes, the IRLC’s sister clade, lack NCRs yet yet include perennial species that maintain productive nodules for decades. Here we identify a nodule-proline-glycine-rich peptide family (NPGs) as a conserved, genus-wide innovation of Robinia. NPGs are highly abundant, localize to the symbiosome membrane, and are intrinsically disordered. Exposure of Mesorhizobium robiniae to recombinant NPGs reprograms physiology, inducing nitrogenase expression while dampening growth without loss of viability. NPGs thus exemplify a distinct, reversible strategy of symbiont control, expanding the peptide repertoire that supports nitrogen fixation in perennial legumes.
2026-05-06 | GSE307062 | GEO
Project description:Backbone phylogeny and evolution of Apioideae (Apiaceae): New insights from phylogenomic analyses of plastome data
Project description:The goal of the study was to test whether CBD103 genotype of North American gray wolves impacts the gene expression response to polyI:C or to live canine distemper virus. We established 24 primary cultures of epidermal keratinocytes from skin punches of North American gray wolves, and also generated an immortalized keratinocyte line and a CRISPR/Cas9 edited cell line. We evaluated the gene expression response of cells to either 24 hours challenge with 1 ug/ml polyI:C or to five days challenge with live canine distemper virus (100 TCID50/ml). Every challenged cell culture had a paired null control sample (plated and collected at same time points).
Project description:Purpose: The goal of the current study was to find the candidate genes responsible for the habita specific clock variation in N. discreta. Methods: We performed RNA-seq experiment using four strains ; African parent (FGSC8831), North American parent (FGSC 8578) and two representative progeny representing African clock phenotype (N309-89) and North American clock phenotype (N309-50). Results: We identified one candidate gene that meets our criteria; confirmed it's expression by qPCR and it's expression pattern is associated with parent genotype. Conclusions: Our approach using the expression profiles and SNP data of two parents and two representative progeny led us to identify a candidate gene for a complex clock adaptation phenotype.
2018-09-18 | GSE120053 | GEO
Project description:Genomic studies of Crimean rare and endemic Apiaceae species
Project description:We measured transcriptional profiles of individuals of Andropogon gerardii, a C4 grass native to North American grasslands, in a field experiment in which both temperature and precipitation have been manipulated to simulate key aspects of forecasted climate change.
