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Arabidopsis thaliana

ABSTRACT: Expression data from 2-week-old Arabidopsis untreated seedlings grown under a short day condition

OTHER RELATED OMICS DATASETS IN: E-GEOD-17499

PROVIDER: PRJNA118923 | ENA |

REPOSITORIES: ENA

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E-GEOD-17499

Project description:Expression data from 2-week-old Arabidopsis untreated seedlings grown under a short day condition

2015-05-15 | E-GEOD-17499 | ExpressionAtlas

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Project description:Methyl CpG Binding (MBD) proteins are a class of protein that binds with methylated DNA. In the model plant Arabidopsis thaliana there are 13 MBD proteins are there. To understand the gene regulation we performed microarray analysis of mutants of three genes (atmbd4, atmbd6 and atmbd11). Ten day old seedlings of these three mutant were compared with the wild type plants grown in same condition.

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Project description:We sequenced the poly(A)+ and poly(A)- samples of the roots and shoots from 10-day-old WT seedlings grown under P+ and P- condition. The WT plant refers to Columbia ecotype Arabidopsis seedlings. Each condition has two replicates. After total RNA extraction, ribosomal RNAs were removed using RiboMinus™ Plant Kit repeated two times. The poly(A)+ and poly(A)- constituent were separated with oligo(dT) magnetic beads (Oligotex mRNA Mini Kit, QIAGEN). Using a 2-fold change and a P-value <0.05 as the cut-off for selecting the differentially expressed transcripts, we globally identified novel noncoding lncRNAs.

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Arabidopsis thaliana

Project description:2-week-old Arabidopsis seedlings (Columbia ecotype)

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Arabidopsis ult1-3 mutant restored LFYasEMF1's phenotype

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Transcript analysis under calcium deficiency in 6 day old Marchantia polymorpha wildtype (Tak-1) and Gβ-null mutant (gpb1-2) gemmalings and inter-species comparison of M. polymorpha, Arabidopsis and lettuce under calcium deficiency

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