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Role of TET3 in mouse embryonic and adult hematopoiesis [EM-Seq]

ABSTRACT: Role of TET3 in mouse embryonic and adult hematopoiesis [EM-Seq]

PROVIDER: PRJNA1190065 | ENA |

REPOSITORIES: ENA

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Role of TET3 in mouse embryonic and adult hematopoiesis

Project description:Role of TET3 in mouse embryonic and adult hematopoiesis

| PRJNA1190062 | ENA

Role of TET3 in mouse embryonic and adult hematopoiesis [EM-Seq]

Project description:In this study: we mapped genome-wide DNA methylation levels in Tet3F/F (control) and cKO Tie2+/cre; Tet3F/F (cKO) Lin– bone marrow cells by enzymatic methyl-sequencing (EM-seq).

2025-04-22 | GSE282718 | GEO

Role of TET3 in mouse embryonic and adult hematopoiesis [E5hmC-seq]

Project description:Role of TET3 in mouse embryonic and adult hematopoiesis [E5hmC-seq]

| PRJNA1190066 | ENA

Role of TET3 in mouse embryonic and adult hematopoiesis [RNA-Seq]

Project description:Role of TET3 in mouse embryonic and adult hematopoiesis [RNA-Seq]

| PRJNA1190067 | ENA

Role of TET3 in mouse embryonic and adult hematopoiesis [RNA-Seq]

Project description:In this study: We identified TET3 target genes by transcriptomic profiling of Tet3F/F and Tie2+/cre; Tet3F/F Lin– bone marrow cells by RNA-sequencing.

2025-04-22 | GSE282719 | GEO

Role of TET3 in mouse embryonic and adult hematopoiesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-04-22 | GSE282720 | GEO

Redirected nuclear glutamate dehydrogenase supplies Tet3 with a-ketoglutarate in neurons

Project description:Tet3 is an Fe2+-dependent enzyme that oxidizes genomic 5-methylcytosine to 5-hydroxymethylcytosine with the help of alpha-ketoglutarate and oxygen. It is the most abundant Tet enzyme in differentiated tissues including brain. Adult brain contains the highest 5-hydroxymethylcytosine levels. How alpha-ketoglutarate is made available for the oxidation of mC in brain cells and how the Tet activity is linked to neural activity are unsolved questions. Our experiments with full mouse brains show that Tet3 interacts in the nucleus directly with selected enzymes of the mitochondrial citric acid cycle. This leads to the formation of isocitrate. Tet3 also interacts with aspartate aminotransferase, which produces oxaloacetate. Although oxaloacetate and isocitrate are biosynthetic alpha-ketoglutarate precursors, they function as inhibitors of Tet3 and are needed to protect the reactive Fe2+ center from degrading DNA. The supply of Tet3 with alpha-ketoglutarate is established by a direct interaction of Tet3 with glutamate dehydrogenase (Glud1), which converts the neurotransmitter glutamate directly into alpha-ketoglutarate. This links Tet3 function to neural activity.

2021-05-10 | PXD004518 | Pride

Tet3 ablation in adult brain neurons increases anxiety-like behavior and regulates cognitive function in mice

Project description:TET3 is a member of the Ten-eleven translocation (TET) family of enzymes which oxidize 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). Tet3 is highly expressed in the brain, where 5hmC levels are most abundant. In adult mice, we observed that TET3 is present in mature neurons and oligodendrocytes but is absent in astrocytes. To investigate the function of TET3 in adult post-mitotic neurons, we crossed Tet3 floxed mice with a neuronal Cre-expressing mouse line, Camk2a-CreERT2, obtaining a Tet3 conditional KO mouse line. Ablation of Tet3 in adult mature neurons resulted in increased anxiety-like behavior with concomitant hypercorticalism, and impaired hippocampal-dependent spatial orientation. Transcriptome and gene-specific expression analysis of the hippocampus showed dysregulation of genes involved in glucocorticoid signaling pathway (HPA axis) in the ventral hippocampus, whereas upregulation of immediate early genes (IEGs) was observed in both dorsal and ventral hippocampal areas. Additionally, Tet3 cKO mice exhibit increased dendritic spine maturation in the ventral CA1 hippocampal subregion. Based on these observations, we suggest that TET3 is involved in molecular alterations, that govern hippocampal-dependent functions. These results reveal a critical role for epigenetic modifications in modulating brain functions, opening new insights into the molecular basis of psychiatric disorders.

2020-01-16 | GSE140850 | GEO

Role of TET3 in mouse embryonic and adult hematopoiesis [E5hmC-seq]

Project description:In this study: we mapped genome-wide DNA hydroxymethylation levels in Tet3F/F (control) and cKO Tie2+/cre; Tet3F/F (cKO) Lin– bone marrow cells by enzymatic 5-hydroxymethylation sequencing (E5hmC-seq).

2025-04-22 | GSE282717 | GEO

TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn

Project description:Ten-eleven-translocation (TET) proteins catalyze DNA hydroxylation, playing an important role in demethylation of DNA in mammals. Remarkably, although hydroxymethylation levels are high in the mouse brain, the potential role of TET proteins in adult neurogenesis is unknown. We show here that a non-catalytic action of TET3 is essentially required for the maintenance of the neural stem cell (NSC) pool in the adult subventricular zone (SVZ) niche by preventing premature differentiation of NSCs into non-neurogenic astrocytes. This occurs through direct binding of TET3 to the paternal transcribed allele of the imprinted gene Small nuclear ribonucleoprotein-associated polypeptide N (Snrpn), contributing to transcriptional repression of the gene. The study also identifies BMP2 as an effector of the astrocytic terminal differentiation mediated by SNRPN. Our work describes a novel mechanism of control of an imprinted gene in the regulation of adult neurogenesis through an unconventional role of TET3.

2019-04-23 | GSE127815 | GEO

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