Impact of TAZ (WWTR1) depletion on gene expression during primary first-trimester extravillous trophoblast differentiation
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ABSTRACT:
PROVIDER: PRJNA1190489 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR31508644.fastq.gz | Fastqsanger.gz | |||
| SRR31508645.fastq.gz | Fastqsanger.gz | |||
| SRR31508646.fastq.gz | Fastqsanger.gz | |||
| SRR31508647.fastq.gz | Fastqsanger.gz | |||
| SRR31508648.fastq.gz | Fastqsanger.gz |
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Project description:Human placental cytotrophoblasts undergo differentiation into extravillous trophoblasts (EVTs) that anchor the placenta to the uterine wall and establish blood flow to the developing fetus. The transcriptional co-activator TAZ (WWTR1) is one of the main effectors of the Hippo signaling pathway, which regulates organ size by controlling important cellular processes such as cell proliferation, apoptosis, stem cell self-renewal and differentiation. Additionally, TAZ is involved in mechano-sensing and cell contact inhibition. In order to gain deeper insights into the function of TAZ in the EVT differentiation process, siRNA (small interfering RNA) treatment was employed to reduce TAZ levels in cytotrophoblasts, which were collected from placentae between 8th and 10th weeks of pregnancy, and underwent EVT differentiation in vitro. The objective of the study was to elucidate the influence of TAZ on the differentiation and function of EVTs by comparing the gene expression profiles of TAZ gene-silenced cells to those with normal TAZ levels.
2025-03-28 | GSE282830 | GEO
Project description:The hippo signaling cofactor TAZ (Also known as WWTR1) regulates expression of genes upon interaction with TEAD transcription factors. Among the TEAD transcription factors, TEAD4 is implicated in regulation of the self-renewal process of human trophoblast stem cells. However, we have a poor understanding about the contribution of TAZ, a co-factor of TEAD4, in that process. Thus, the goal of this study is to define the importance of TAZ in orchestrating global gene expression program in human trophoblast stem cells. Using a RNA interference strategy (RNAi), we depleted TAZ expression in human trophoblast stem cells and compared global gene expression patterns via RNA sequencing (RNA-seq). Three individual experiments were performed. The submitted files contain the data (raw fastq files, count data and deseq2 output) from those experiments.
2022-08-08 | GSE188738 | GEO
Project description:Uterine Natural Killer (uNK) cells regulate essential developmental processes at the maternal-fetal interface during early pregnancy. Uterine NK cell functions are tightly regulated during early placentation to stimulate trophoblast invasion and remodel uterine spiral arteries. Access to human 1st and 2nd trimester uterine tissues allowed us to investigate the transcriptional changes in uNK cells during the early stages of early human pregnancy. Microarray analysis identified 97 upregulated genes in 2nd compared to 1st trimester purified uNK cells of which the majority (61%) clustered as interferon-stimulated-genes (ISG), with ISG15 and ISG20 being upregulated profoundly. Type I interferons (IFNα/β), but not type II interferon (IFNɣ) increased expression of the identified interferon target genes ISG15 and ISG20 in uNK cells in vitro. Moreover, the cytokine-like protein ISG15 stimulated in vitro trophoblast invasion. Second trimester uNK cells promoted trophoblast invasion in vitro, whereas both 1st and 2nd trimester uNK cells stimulated endothelial tube formation. IFNα but not IFNβ stimulation of 1st trimester uNK cells enhanced their capacity to promote trophoblast invasion. In conclusion, the uNK cell interferon transcriptome is upregulated during the 2nd trimester allowing uNK cells to promote trophoblast invasion. Type I interferon signaling regulates uNK cell-induced trophoblast invasion via induction of effector molecules like ISG15. First trimester uNK cells can be induced to act like second trimester uNK cells by IFNα with respect to promotion of trophoblast invasion. Key words: Gene expression profiling ■ uterine natural killer cells ■ extravillous trophoblast invasion ■ interferon alpha ■ ISG15
2020-01-30 | GSE64898 | GEO
Project description:During placentation, placental cytotrophoblast cells differentiate into syncytiotrophoblast cells and extravillous trophoblast cells. In placenta, the expression of various genes is regulated by the Hippo pathway through the transcriptional coactivator YAP/TAZ-TEAD activity. To examine the effect of YAP/TAZ and/or TEAD on trophoblast differentiation, knockdown experiments were performed. Microarray analysis were performed to identify YAP/TAZ and/or TEAD target genes in human trophoblast.
2022-07-17 | GSE182900 | GEO
Project description:Gene expression analysis of extravillous trophoblast cells during the first-trimester
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Project description:To better understand how DNA methylation influences placentation, DNA from first trimester primary trophoblast populations (side-population trophoblasts, cytotrophoblasts and extravillous trophoblasts) isolated using FACS underwent reduced representation bisulfite sequencing and were compared to publicly available data of blastocyst-derived and somatic cell populations.
2018-12-25 | GSE109682 | GEO
Project description:We used adenoviral-mediated overexpression of MYC-BioID2, MYC-BioID2-SKI, MYC-BioID2-WWTR1 (TAZ) in human primary cardiac fibroblasts to elucidate the interaction between SKI and the Hippo signaling pathway. Original data is also available on the Global Proteome Machine (http://hs2.proteome.ca/tandem/thegpm_tandem.html). Datasets are identified as follows: GPM10000002938 and 2939 are untreated negative control cell lysates; GPM10000002941 and 2942 are "empty" MYC-BioID2 vector; GPM10000002943 and 2944 are MYC-BioID2-SKI; and GPM10000002944 and 2945 are MYC-BioID2-WWTR1(TAZ).
2021-09-09 | PXD018246 | Pride
Project description:The transcriptional co-activator TAZ/WWTR1 plays a central role in the Hippo signaling pathway and acts as crucial mediator in maintaining organ size and tissue homeostasis. Changes in its activity can lead to diseases, including cancer, due to uncontrolled cell growth or aberrant cellular behavior. The aim of this study was to identify gene signatures that are specifically regulated by TAZ in human trophoblast cells. This was achieved by comparing JEG-3 wild-type and TAZ knockout choriocarcinoma cells, generated with CRISPR-Cas9 technology.
2025-03-28 | GSE282831 | GEO
Project description:TAZ, also known as WWTR1, is the one of the effectors of Hippo pathway. With its paralog, YAP, TAZ promotes organ size growth as well as tumor metastasis. In human renal carcinoma cells, we found that TAZ-silencing induces resisntance toward erastin-induced ferroptosis. In this study, TAZ is silencing in clear cell carcinoma cell line RCC4 to elucidate the downstream targets that promotes the resistance toward erastin-induced ferroptosis.
2019-09-03 | GSE121689 | GEO
Project description:Purpose: During early placentation, extravillous trophoblast (EVT) cell invasion into maternal decidua plays a crucial role in establishment of the successful pregnancy. However, little is known about the characteristic features of EVT-associated ncRNAs. The purpose of this study is to elucidate gene expression profile for both coding and non-coding genes (i.e., mRNAs, lncRNAs, and miRNAs) expressed in EVT cells isolated from the first-trimester human placenta by RNA sequencing analysis. Methods: Human first trimester placental tissues (at gestational week 7, n=3) were obtained after legal abortions. EVT cells growing from explanted human chorionic villi were isolated. Minced chorionic villous tips were defined as first-trimester chorionic villous trophoblast (CVT) cells. Using total RNAs of these samples, mRNA/lncRNA-sequencing analysis was carried out on an Illumina MiSeq (San Diego, California, USA) using MiSeq v2 Reagent Kit 300cycle (cat. no. MS-102-2002; Illumina) and MiSeq v2 Reagent Kit 50cycle (cat. no. MS-102-2001; Illumina), respectively. The paired-end reads of 151 bp length for mRNA/lncRNA-sequencing and single reads of 26 bp length for miRNA-sequencing were obtained as fastq files which were imported into CLC genomics workbench (version 8.0.1; Qiagen, Venlo, Netherlands). Clean reads were aligned to the human genome sequence retrieved from the Ensembl database (Assembly GRCh38.p13, database version 82.38 and 101.38) and microRNA database miRbase (release 21), using CLC Genomics Workbench Map Reads to Reference tool. Normalization of gene expression data was performed by the TMM method of edgeR package. Statistically differentially expressed transcripts were conducted using edgeR. Genes that had a false discovery rate (FDR)-corrected p-value (q-value) of < 0.05 and an absolute log2 fold change (log FC) ≥ 1 were judged to be significantly differentially expressed (i.e., differentially expressed genes, DEGs). Results: Five thousand ninty-two mRNAs , 422 lncRNAs and 400 miRNAs were found to be differentially expressed. One hundred differentially expressed genes (i.e., 10 mRNAs, 7 lncRNAs, and 83 miRNAs) that are located in the chromosome 14q32.2 region containing the eutherian-specific imprinted DLK1-DIO3 domain were significantly down-regulated in EVT cells. In terms of imprinted genes (http://www.geneimprint.com/site/genes-by-species), 68 genes were found to be differentially expressed; 71% of these genes were down-regulated in EVT cells. lncRNA H19 was a DEG (q = 1.29.E-09, log FC = 2.98) and the most highly expressed lncRNA (70.47%) in EVT cells. Conclusions: The present research should be valuable for a resource for future ncRNA research in EVT cells.
2021-02-07 | GSE163651 | GEO