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ChIP-seq of H2A H2A.Z H2A.W H3 in Col-0, h2a, h2a.z, and h2a.w ChIP-seq of H3K36me3 H3K9me2 in Col-0

ABSTRACT: ChIP-seq of H2A H2A.Z H2A.W H3 in Col-0, h2a, h2a.z, and h2a.w ChIP-seq of H3K36me3 H3K9me2 in Col-0

PROVIDER: PRJNA1191503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31537800_1.fastq.gz	Fastqsanger.gz
	SRR31537800_2.fastq.gz	Fastqsanger.gz
	SRR31537801_1.fastq.gz	Fastqsanger.gz
	SRR31537801_2.fastq.gz	Fastqsanger.gz
	SRR31537802_1.fastq.gz	Fastqsanger.gz

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ChIP-seq of H2A H2A.Z H2A.W H3 in Col-0, h2a, h2a.z, and h2a.w; ChIP-seq of H3K36me3 H3K9me2 in Col-0

Project description:ChIP-seq of H2A H2A.Z H2A.W H3 in Col-0, h2a, h2a.z, and h2a.w; ChIP-seq of H3K36me3 H3K9me2 in Col-0

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MNase-seq of seedlings in Arabidopsis thaliana Col and h2a.w mutants

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Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone H2A variants and histone modifications in WT Col-0 seedlings.

Project description:How histone intrinsic sequence variation or regulatory modifications regulate nucleosome interactions with transcription remain unclear. To clarify this question, we examined how histone variants and histone modifications assemble in the Arabidopsis thaliana genome, identifying a limited number of chromatin states that divide euchromatin and heterochromatin in biologically significant subdomains. We showed that histone variants were as significant as histone modifications to determine the composition of chromatin states. The loss of function of the chromatin remodeler DECREASED IN DNA METHYLATION (DDM1) prevented the exchange between the histone variants H2A.Z and H2A.W over transposons resulting in their enrichment in chromatin states found only on proteins coding genes in the wild type. Hence, the dynamics of histone H2A variants exchange impacted the definition and distribution of chromatin states. We propose that dynamics of histone variants control the organization of histone modifications into chromatin states to achieve landmarks that signify the ability for transcription. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone H2A variants and histone modifications in seedlings .

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Total RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0 in Arabidopsis [msh1_graft_leaf_RNA-seq]

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RNA-seq for Col-0 WT, ddm1(-/-) and Col-0 X ddm1 (F1) to evaluate transposon expression in F1 Col-0Xddm1 and ddm1

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Bisulfite (methylome) sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_BS-seq]

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