Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Anitbody affinity birth through somatic hypermutation

ABSTRACT: Anitbody affinity birth through somatic hypermutation

PROVIDER: PRJNA1191854 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31543360.fastq.gz	Fastqsanger.gz
	SRR31543361.fastq.gz	Fastqsanger.gz
	SRR31543362.fastq.gz	Fastqsanger.gz
	SRR31543363.fastq.gz	Fastqsanger.gz
	SRR31543364.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Somatic hypermutation generates antibody specificities beyond the primary repertoire

Project description:B cell somatic hypermutation (SHM) and selection in germinal centers (GCs) enhance antibody affinity for antigen. Here, we investigated whether SHM-based antibody evolution is restricted to specificities established through V(D)J recombination in the primary repertoire. Tracking pre-defined non-specific B cells across multiple immunization models revealed that non-cognate B cells within GCs undergo SHM. Under conditions of limited B cell competition, these B cells generated de novo antigen recognition to multiple epitopes across diverse model antigens. Phylogenetic analyses identified diverse mutational pathways leading to new antigen affinities, and enhanced T cell co-stimulation further promoted new antigen recognition. Our data support a model in which B cell competition-rather than an intrinsic requirement for specific affinity-limits the emergence of new affinities through SHM, highlighting the mammalian adaptive immune system's ability to explore antibody-antigen interactions beyond those encoded by the V(D)J-dependent primary repertoire, demonstrating the flexibility of SHM in not only ripening but also reshaping specificity.

2024-12-04 | GSE283094 | GEO

UNG shapes the specificity of AID-induced somatic hypermutation

Project description:This SuperSeries is composed of the following subset Series: GSE39108: UNG shapes the specifity of AID-induced somatic hypermutation in non B cells GSE39114: UNG shapes the specifity of AID-induced somatic hypermutation in B cells Refer to individual Series

2012-07-04 | E-GEOD-39115 | biostudies-arrayexpress

Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effect

Project description:Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effect

| PRJNA1259990 | ENA

Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effect [scRNA-Seq]

Project description:Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effect [scRNA-Seq]

| PRJNA1284894 | ENA

Mapping and Analysis of Caenorhabditis elegans Transcription Factor Binding Specificities

Project description:The nematode Caenorhabditis elegans is a powerful model for studying gene regulation, as it has a compact genome and a wealth of genomic tools. However, identification of regulatory elements has been hampered by the fact that DNA binding motifs are known for only 71 (9%) of the estimated 763 high-confidence sequence-specific transcription factors (TFs). To address this problem, we performed protein binding microarray (PBM) experiments on representatives of canonical TF families in the C. elegans TF repertoire, obtaining motifs for 129 distinct TFs. Moreover, we can infer motifs for 97 additional TFs that have DNA binding domains that are very similar to those already characterized, resulting in a total coverage of binding specificities for almost 40% of the C. elegans TF repertoire. These data highlight the diversification of binding motifs for the nuclear hormone receptor (NHR) and C2H2 zinc finger families, and reveal unexpected diversity of motifs for others, including the T-box and DM families. Enrichment of motifs in the promoters of functionally related genes is consistent with known biology in many cases, and also identifies putative new regulatory roles for poorly characterized TFs. The motifs are available at http:// http://cisbp.ccbr.utoronto.ca.

2015-04-23 | GSE65719 | GEO

Mapping and Analysis of Caenorhabditis elegans Transcription Factor Binding Specificities

2015-04-23 | E-GEOD-65719 | biostudies-arrayexpress

Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effect [scRNA-Seq 2]

Project description:Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effect [scRNA-Seq 2]

| PRJNA1284896 | ENA

Addressing the repertoire of T helper Cells in the context of myocardial infarction

Project description:T-cells can influence the post-myocardial infarction healing process. The fundamental role of T cells in adaptive immunity relies mainly on a diverse repertoire of T cell receptor (TCR) α and β chains that display unique antigen specificities. Thus, we performed high-throughput sequencing of the TCRβ chains on CD4+ T cells purified by fluorescence-activated cell sorting (FACS) from the heart and med-LNs of MI and sham-operated mice as an unbiased approach to screen for MI-related TCR signatures.

2019-08-14 | GSE117076 | GEO

Independent roles of switching and hypermutation in the development and persistence of B lymphocyte memory [Nurr77]

Project description:Somatic hypermutation (SHM) and class switch recombination (CSR) increase the affinity and diversify the effector functions of antibodies during immune responses. Although SHM and CSR are fundamentally different, their independent roles in regulating B cell fate have been difficult to uncouple because a single enzyme, activation-induced cytidine deaminase (encoded by Aicda), initiates both reactions. Here, we used a combination of Aicda and antibody mutant alleles that separate the effects of CSR and SHM on polyclonal immune responses. We found that class-switching to IgG1 biased the fate choice made by B cells, favoring the plasma cell over memory cell fate without significantly affecting clonal expansion in the germinal center (GC). In contrast, SHM reduced the longevity of memory B cells by creating polyreactive specificities that were selected against over time. Our data define the independent contributions of SHM and CSR to the generation and persistence of memory in the antibody system.

2016-10-19 | GSE76865 | GEO

Independent roles of switching and hypermutation in the development and persistence of B lymphocyte memory [IgM_IgG1]

2016-10-19 | GSE76864 | GEO