Project description:Untargeted metabolomics analysis of cecal contents from the Siberian chipmunk (Tamias sibiricus) across four physiological stages of hibernation: pre-hibernation (preH), deep torpor (torpor), interbout arousal (IBA), and post-hibernation (postH). The study aims to characterize the functional reprogramming of the gut metabolome during hibernation.
Study Design:
Species: Tamias sibiricus
Sample Type: Cecal contents
Groups: 4 groups (preH, torpor, IBA, postH) with 5 biological replicates per group (Total 20 samples).
Method: Samples were analyzed using LC-MS/MS on a Thermo UHPLC-Q Exactive HF-X system in both positive and negative ion modes.
Related Manuscript: Combining gut metatranscriptome and metabolome reveals metabolic adaptation of the food-storing Siberian chipmunk (Tamias sibiricus) to hibernation.
