Project description:Transcriptional profiling of HMEC-1 cells comparing untreated control cells with TNF-alpha-treated cells. Goal was to include more evidence by adding another research method. Keywords: Inflammatory stimulation
Project description:Cells were treated with Interferon at concentrition of 1000IU/ml and the non-treated cells were used as control. Samples with 4h, 8h, and 24h of Interferon treatment were collected. Then the cells were stored at -80 centigrade.
Project description:CUT&RUN on Spen-degron TX1072 mESCs was performed in 2 biological replicates. Cells were treated with doxycycline (1ug/mL) for 0h, 4h, 8h, 24h and 8h doxycycline and auxin (500uM).
Project description:To investigate effects of 4 mixture of cytokines including TNF-alpha, IL-1beta IFN-gamma, and TGF-bata1 on rhematoid arthritis synovial fibroblasts (RASFs), we treated RASFs (n = 3) with TNF-alpha (1 ng/mL), IL-1beta (0.1 ng/mL), IFN-gamma (10 ng /mL), TGF-beta1 (1 ng/mL) (4mix) or vehicle control (Veh) for 24 hours and conducted RNA-seq analysis.
Project description:TNFa stimulated human cultured podocytes compared with unstimulated (vehicle control (VC)) podocytes. Incubation of podocytes with high concentrations of TNF alpha led to an overexpression of ICAM1 in particular. We studied this system as a comparator for organoids also stimulated with the same concentration of TNFa. Four conditions (6 replicates each) 1) treatment (5 ng/mL TNFa) for 24 hours 2) control (PBS) for 24h 3) treatment (5 ng/mL TNFa) for 48 hours 4) control (PBS) for 48h
Project description:TNFa stimulated human cultured podocytes compared with unstimulated (vehicle control (VC)) podocytes. Incubation of podocytes with high concentrations of TNF alpha led to an overexpression of ICAM1 in particular. We studied this system as a comparator for organoids also stimulated with the same concentration of TNFa. Four conditions (6 replicates each) 1) treatment (5 ng/mL TNFa) for 24 hours 2) control (PBS) for 24h 3) treatment (5 ng/mL TNFa) for 48 hours 4) control (PBS) for 48h
Project description:Effect of TNF-alpha on microRNAs levels in Human Umbilical Endothelial Cells (HUVECs). HUVEC that were treated or not for 2 or 24 hours with TNF (10 ng/ml). Duplicate samples (1 or 2) of two different isolations of HUVEC (A or B)
Project description:HMEC cultures were left untreated or stimulated for 5h with 2 ng/ml TNF. Comparison of the gene expression profiles revealed the TNF-mediated gene expression changes. Keywords: parallel sample
