Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1195537 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Overexpression of OsMYB103L leads to leaf rolling in transgenic rice plants. To explore the possible molecular mechanism of OsMYB103L’s effects on rice leaf development, we examined the expression profiles of OsMYB103L overexpression transgenic rice plants and wild type leaf blades using Digital Gene Expression (DGE) profiling analysis.
2016-11-13 | GSE52394 | GEO
Project description:To understand downstream genes affected by overexpression of OsbHLH148, the Rice 3’-Tiling Microarray analysis (GreenGene Biotech, Yongin, Korea) was carried out to profile gene expression of OsCc1:OsbHLH148 transgenic plants in comparison with wild-type plants under normal growth conditions. RNA samples from these plants were used to generate cyanine-3 (Cy3)-labeled complementary DNA (cDNA) probes, which were then hybridized to the microarray. Each data set was obtained from two biological repeats with independent transgenic lines.
2011-01-01 | GSE22676 | GEO
Project description:To identify genes involved in the OsDIS1-mediated drought-responsive pathway, we performed microarray analysis of the OsDIS1 overexpression and wild-type plants under both normal and drought stress conditions using the Agilent rice Genechip. Seven-day-old plants of the OsDIS1 overexpression line 9-4-2 as well as the wild-type plants were used in the drought treatment. OsLEA3 was used as a positive control for the drought treatment. Genes with more than two-fold changes in the overexpression plants compared with the wild-type plants were selected. The expression pattern of some differentially expressed genes was further confirmed by real-time PCR.
2012-01-01 | GSE30151 | GEO
Project description:High mobility group (HMG) proteins play an important role in regulation of gene transcription through modulate the structure of DNA. In this study, OsHMGB707, a HMG gene localized in rice drought resistance QTL interval, was isolated and the function on rice stress resistance was identified. Overexpression of OsHMGB707 significantly enhanced the drought resistance of the transgenic rice plants, whereas the OsHMGB707-RNAi transgenic rice plants exhibited slightly decrease in drought stress tolerance. To search the downstream genes regulated by OsHMGB707, we performed microarray analysis of the OsHMGB707-overexpressing, OsHMGB707-RNAi and wild-type plants under both normal conditions using Affymetrix Rice Genome Genechip. 21-day-old plants of the OsHMGB707-overexpressing line OE1, OsHMGB707-RNAi line RNAi1 as well as the wild-type plants were used in the normal condition.
2019-05-08 | GSE130821 | GEO
Project description:Several homeobox genes belonging to HD-ZIP I subfamily are highly induced by drought stress at various developmental stages in rice. To analyze the role of a candidate HD-ZIP I subfamily member, OsHOX24, we constitutively overexpressed it in rice. The physiological analyses revealed that overexpression of OsHOX24 gene reduced drought stress tolerance in transgenic plants as compared to wild-type. We used microarrays to study the global effect of OsHOX24 overexpression in rice as compared to wild-type under control and drought stress condition.
2017-06-12 | GSE79212 | GEO
Project description:Jasmonates (JA) and abscisic acid (ABA) are phytohormones known to play important roles in plant response and adaptation to various abiotic stresses including salinity, drought, wounding, and cold. JAZ (JASMONATE ZIM-domain) proteins have been reported to play negative roles in JA signaling. However, direct evidence is still lacking that JAZ proteins regulate drought resistance. In this study, OsJAZ1 was investigated for its role in drought resistance in rice. Expression of OsJAZ1 was strongly responsive to JA treatment, and it was slightly responsive to ABA, salicylic acid, and abiotic stresses including drought, salinity, and cold. The OsJAZ1-overexpression rice plants were more sensitive to drought stress treatment than the wild-type rice Zhonghua 11 (ZH11) at both the seedling and reproductive stages, while the jaz1 T-DNA insertion mutant plants showed increased drought tolerance compared to the wild-type plants. The OsJAZ1-overexpression plants were hyposensitive to MeJA and ABA, whereas the jaz1 mutant plants were hypersensitive to MeJA and ABA. In addition, there were significant differences in shoot and root length between the OsJAZ1 transgenic and wild-type plants under the MeJA and ABA treatments. A subcellular localization assay indicated that OsJAZ1 was localized in both the nucleus and cytoplasm. Transcriptome profiling analysis by RNA-seq revealed that the expression levels of many genes in the ABA and JA signaling pathways exhibited significant differences between the OsJAZ1-overexpression plants and wild-type ZH11 under drought stress treatment. Quantitative real-time PCR confirmed the expression profiles of some of the differentially expressed genes, including OsNCED4, OsLEA3, RAB21, OsbHLH006, OsbHLH148, OsDREB1A, OsDREB1B, SNAC1, and OsCCD1. These results together suggest that OsJAZ1 plays a role in regulating the drought resistance of rice partially via the ABA and JA pathways.
2018-02-28 | GSE107425 | GEO
Project description:Membraneless biomolecular condensates formed through liquid-liquid phase separation (LLPS) are known to play key roles in plant growth and stress responses. How plant viruses utilize LLPS to escape host immunity remains largely unexplored. Here, we present evidence demonstrating that the P6 protein encoded by southern rice black-streaked dwarf virus (SRBSDV) undergoes LLPS. P6 interacts with OsTSN1 and triggers the assembly of P6-OsTSN1-containing droplets that co-localize with stress granules (SGs). Within these P6-OsTSN1-containing droplets, P6 enhances the nuclease activity of OsTSN1 via promoting its multimerization to degrade transcripts with G-A-rich motifs of two transcription factors (TFs), OsNAC15 and OsLHY. These TFs regulate the transcription of OsJAZ6, OsJAZ12, and OsATG8C, involved in jasmonic acid (JA)- and autophagy-associated defense pathways in plants. Additionally, the degradation of OsNAC15 and OsLHY transcripts in the P6-OsTSN1-containing droplets in SGs weakens JA- and autophagy-mediated defenses in rice, facilitating SRBSDV infection. Interestingly, similar to SRBSDV P6, intrinsically disordered region (IDR)-containing RNA silencing suppressors encoded by other rice viruses such as rice black-streaked dwarf virus and rice stripe virus, also interact with OsTSN1, promoting the degradation of OsNAC15 and OsLHY transcripts to enhance viral infection. Our findings indicate that OsTSN1 acts as a central positive regulator of virus infection in rice, convergently co-opted by viruses. These insights help us to better understand the roles of LLPS and OsTSN1 in virus infection in rice.
2025-07-07 | GSE283861 | GEO
Project description:Membraneless biomolecular condensates formed through liquid-liquid phase separation (LLPS) are known to play key roles in plant growth and stress responses. How plant viruses utilize LLPS to escape host immunity remains largely unexplored. Here, we present evidence demonstrating that the P6 protein encoded by southern rice black-streaked dwarf virus (SRBSDV) undergoes LLPS. P6 interacts with OsTSN1 and triggers the assembly of P6-OsTSN1-containing droplets that co-localize with stress granules (SGs). Within these P6-OsTSN1-containing droplets, P6 enhances the nuclease activity of OsTSN1 via promoting its multimerization to degrade transcripts with G-A-rich motifs of two transcription factors (TFs), OsNAC15 and OsLHY. These TFs regulate the transcription of OsJAZ6, OsJAZ12, and OsATG8C, involved in jasmonic acid (JA)- and autophagy-associated defense pathways in plants. Additionally, the degradation of OsNAC15 and OsLHY transcripts in the P6-OsTSN1-containing droplets in SGs weakens JA- and autophagy-mediated defenses in rice, facilitating SRBSDV infection. Interestingly, similar to SRBSDV P6, intrinsically disordered region (IDR)-containing RNA silencing suppressors encoded by other rice viruses such as rice black-streaked dwarf virus and rice stripe virus, also interact with OsTSN1, promoting the degradation of OsNAC15 and OsLHY transcripts to enhance viral infection. Our findings indicate that OsTSN1 acts as a central positive regulator of virus infection in rice, convergently co-opted by viruses. These insights help us to better understand the roles of LLPS and OsTSN1 in virus infection in rice.
2025-07-07 | GSE283860 | GEO
Project description:OsTMF is a transcription factor and negatively regulates cold tolerance We used microarray analysis to find differentially expressed genes in OsTMF overexpression (OsTMF-OE) plants compared with the wild-type rice ZH11.
2019-12-31 | GSE129481 | GEO
Project description:miR6024 overexpression may lead to changes in the transcriptome profile of tomato plants. Further changes may be noticed on infecting these plants with the necrotrophic pathogen Alternaria solani. These changes can only be gauged by carrying out a comparative transcriptome analysis with the wild type plants under similar conditions. We have used tomato (Pusa Ruby) for generation of miR6024 overexpressing transgenics. Disease study on these plants were carried out with the necrotrophic fungus A. solani. We carried an RNA-seq analysis using Illumina hiseq sequencing of 5 RNA libraries created from leaf tissues of wild type, OVX6024 transgenics and A. solani infected wild type and OVX6024 plants. The analysis revealed that 334 and 781 genes were significantly regulated in the transgenic plants and the infected transgenic plants respectively, with respect to their suitable wild type controls. GO enrichment analysis and pathway analysis have been carried out as well. This work is supported by grants from DBT and SERB, GoI.
2021-07-02 | GSE166013 | GEO