Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Caenorhabditis elegans

ABSTRACT: Single-Nucleus Neuronal Transcriptional Profiling of Male C. elegans Uncovers Regulators of Sex-Specific and Sex-Shared Behaviors

PROVIDER: PRJNA1195922 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR34148065_1.fastq.gz	Fastqsanger.gz
	SRR34148065_2.fastq.gz	Fastqsanger.gz
	SRR34146787_1.fastq.gz	Fastqsanger.gz
	SRR34146787_2.fastq.gz	Fastqsanger.gz
	SRR34146788_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

A Blueprint of Sex-Specific Neuronal Regulation in the C. elegans Nervous System at Single-Cell Resolution

Project description:Sex-specific behaviors within a species are often attributed to variances in neuronal wiring and molecular signatures. However, how the genetic sex shapes the molecular architecture of the nervous system at a single neuron level is still unknown. To address this gap, we used single-cell RNA-sequencing (scRNA-seq) to profile the transcriptome of the sex-shared nervous system of adult male and hermaphrodite Caenorhabditis elegans. By ranking neurons based on the degree of molecular dimorphism, we uncover novel sexually dimorphic neurons such as PLM neurons, where functional dimorphism was corroborated by diminished posterior touch responses in hermaphrodites. We identify sex-specific regulators of mechanosensory behavior and neuronal function by combining our dataset with reverse genetic screens. While most sex-shared neurons retain their neurotransmitter identity, the male neuropeptide connectome undergoes substantial remodeling, with most of the neuropeptides showing a male-bias expression. This reinforces the notion that neuropeptides are crucial for diversifying connectome outputs. Furthermore, by correlating gene expression with outgoing synaptic connectivity, we identified regulatory candidates for synaptic wiring, including both shared and sex-specific genes. This comprehensive resource provides foundational insights into the molecular drivers of sexual dimorphism, facilitating future exploration of regulatory mechanisms and their impact on sex-specific behaviors in higher organisms.

2025-01-12 | GSE286418 | GEO

Caenorhabditis elegans

Project description:Age-related behaviors have distinct transcriptional profiles in C. elegans

| PRJNA113579 | ENA

Caenorhabditis elegans

Project description:Caenorhabditis elegans Neuronal Cells RNA sequence data

| PRJNA938805 | ENA

Caenorhabditis elegans

Project description:A sex chromosome piRNA promotes robust dosage compensation and sex determination in C. elegans

| PRJNA433627 | ENA

Caenorhabditis elegans

Project description:Function of a sex-chromosome derived piRNA inCaenorhabditis elegans

| PRJNA398958 | ENA

Caenorhabditis elegans

Project description:Microarray profiling, C elegans: Neuronal miRISC IP vs Total RNA

| PRJNA196523 | ENA

Caenorhabditis elegans

Project description:Uncoupling X chromosome number from sex determination separates contribution of sex and X dose to sex-biased gene expression in C. elegans

| PRJNA311565 | ENA

Caenorhabditis elegans

Project description:Adult C. elegans neuronal XBP-1s dependent transcriptional changes in the intestine

| PRJNA482604 | ENA

Sex-Specific Developmental Gene Expression Atlas Unveils Dimorphic Gene Networks in C. elegans

Project description:Sex-specific traits and behaviors are facilitated by the acquisition of unique properties in the nervous system of each sex during development and continues in adulthood. However, the genetic events responsible for introducing these sex-specific features remain poorly understood. In this study, we created a comprehensive gene expression atlas for both sexes of the nematode Caenorhabditis elegans across development. By comparing the transcriptome of pure populations of hermaphrodites and males from early larval stages to adulthood, we discovered numerous differentially expressed genes, including neuronal gene families like transcription factors, neuropeptides, and GPCRs. We identified INS-39, an insulin-like peptide, as a prominent male-biased gene expressed specifically in ciliated sensory neurons. We show that INS-39 serves as an early-stage male marker, facilitating the effective isolation of males in high-throughput experiments. Analysis of ins-39 knock out animals revealed pleiotropic sex-specific roles in temperature sensation, survival in cold temperatures, resilience against high hydrogen peroxide levels, and dauer entry, while also playing a shared, dimorphic role in early life stress. This study offers a comparative sexual and developmental gene expression database for C. elegans, which will facilitate research into the genetic regulation of the sexual development of other organisms. Furthermore, it highlights conserved candidate genes that may underlie the sexually-dimorphic manifestation of different human diseases

2024-03-17 | GSE237937 | GEO

Caenorhabditis elegans

Project description:Contrasting invertebrate immune defense behaviors caused by a single gene, the Caenorhabditis elegans neuropeptide receptor gene npr-1

| PRJNA257404 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data