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Meiotic time course: open chromatin and expression profile

ABSTRACT: Meiotic time course: open chromatin and expression profile

PROVIDER: PRJNA119599 | ENA |

REPOSITORIES: ENA

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Meiotic time course: open chromatin and expression profile

Project description:This SuperSeries is composed of the following subset Series: GSE16163: Meiotic time course of open chromatin as measured by FAIRE GSE18181: Meiotic time course expression profile Refer to individual Series

2009-09-25 | E-GEOD-18256 | biostudies-arrayexpress

Meiotic time course of Histone H3 occupancy

2008-11-01 | GSE10836 | GEO

Meiotic time-course expression profile

Project description:To investigate the dynamics of the meiotic transcriptome and to ensure meiotic synchrony of our samples, we measured RNA abundance in vegetative cells, respiratory (pre-meiotic) cells and in synchronously sporulating cells at various times after transfer to sporulation media (SM). SK1 yeast strain SHy002 (MATa/MATÎ± ho::LYS2/ho::LYS2 leu2::hisG/leu2::hisG lys2/lys2 ura3/ura3) Meiotic Time Course with samples collected during vegetative growth (YPD), respiratory growth (pre-meiotic/YPA 8 hours) and 0 hr, 1.5hr, 3hr, 4.5hr, 6hr, 9hr and 12hr after transfer to sporulation media (SM). 3 separate biological replicates were used for each condition/time-point. Total RNA from each sample was hybridized competitively with a genomic DNA common reference. The expression analysis was carried out from the same biological samples as the FAIRE analysis.

2010-05-22 | E-GEOD-18181 | biostudies-arrayexpress

Meiotic time course of Histone H3 occupancy

Project description:To study the meiotic dynamic of the methylation of H3K4, in relation with the transcriptomic regulation, we determine the meiotic time-course of H3 occupancy by ChIP-chip. Keywords: ChIP-chip, meiotic time course, histone modification Strain ORD7339 meiotic time-course from 0 to 6h. Immunoprecipitation with rabbit polyclonal anti H3Cter (Abcam #1791)

2010-05-17 | E-GEOD-10836 | biostudies-arrayexpress

Meiotic time course of open chromatin as measured by FAIRE

Project description:To investigate the relationship between chromatin organization and meiotic processes, we used Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) to map open chromatin during the transition from mitosis to meiosis in the budding yeast Saccharomyces cerevisiae.

2009-07-01 | GSE16163 | GEO

Meiotic time course of Histone H3 occupancy in a mutant Spo11F

Project description:To study the meiotic dynamic of the methylation of H3K4, in relation with the transcriptomic regulation, we determine the meiotic time-course of H3 occupancy by ChIP-chip in a mutant Spo11F. Keywords: ChIP-chip, meiotic time course, histone modification

2008-11-01 | GSE10838 | GEO

WT_SK1 meiotic time course on expression/splicing microarrays

Project description:synchronized WT_SK1 meiotic time course The SK1 strain background undergoes meiosis with high efficiency and synchronicity (ATCC:MYA-2089)

2011-04-05 | GSE24681 | GEO

Meiotic time course of the trimethylation of the Lysine 4 of Histone H3 (H3K4me3)

Project description:To study the meiotic dynamic of the methylation of H3K4, in relation with the transcriptomic regulation, we determine the meiotic time-course of H3K4me3 by ChIP-chip. Keywords: ChIP-chip, meiotic time course, histone modification

2008-11-01 | GSE10840 | GEO

Meiotic time-course expression profile

2009-09-23 | GSE18181 | GEO

time course expression profile of ap1-cal after induction of an ap1-transgene-Time course of meiotic gene expression in Arabidopsis

Project description:rs11-09_meotic - time course expression profile of ap1-cal after induction of an ap1-transgene - CDKs are major regulators of the mitotic as well as the meiotic cell cycle. In comparison with the mitotic cell cycle much less is known about the regulation of meiosis, especially in plants. One of the reasons for this is the very low abundance and the difficult accessibility of cell undergoing meiosis. We have developed a system to enrich for meiocytes. This will be the material with which we will search for CDK substrates in a biochemical approach. To first get an overview about the meiotic genes expressed in this system, we want to perform here a time series at four time points after the induction that leads to the synchronized development of meiocytes. The in the microarray identified genes, will then set the frame for the upcoming biochemical experiments. - An ap1-cal double mutants carrying an Glucocorticoid-receptor fusion to AP1 was indcued and samples were collected 2 days after induction (dai), 8dapi, 9dai, 10dai and 11dai. Then, the expression profile of 2dai were compared with 8dai, 8 with 9, 9 with 10, and 10 with 11.

2013-02-01 | GSE43646 | GEO

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