Project description:The alkaliphilic halotolerant bacterium Bacillus sp. N16-5 often faces salt stress in its natural habitats. One-color microarrays was used to investigate transcriptome expression profiles of Bacillus sp. N16-5 adaptation reactions to prolonged grown at different salinities (0%, 2%, 8% and 15% NaCl) and the initial reaction to suddenly alter salinity from 0% to 8% NaCl.
Project description:Numerous non-mycorrhizal fungi have been reported to form close symbiotic relationships with plants. These tight mutual relationships can provide physiological benefits to the interacting organisms, including the improvement of nutrient assimilation or enhanced stress tolerance. The root-colonizing fungus Penicillium sp. strain D7 has been isolated from roots of Digitalis thapsi L. that were grown on the As-rich tailings of the Mónica mine in Bustaviejo, Spain. Here, we report the promotion of plant biomass production and stress tolerance of Arabidopsis thaliana L. plants inoculated with D7. The comparative analysis of the effect of mock- and D7-inoclulated wild-type Arabidopsis plants (Col-0) in the absence and presence of arsenite As(III) stress conditions (15 µM NaAsO2). Our data provide evidence for a profound effect of D7 on As(III) detoxification through the activation of a regulatory network that includes several plant hormones. In addition, fungus inoculation resulted in a significant induction of As sequestration in the root, which allowed for an enhanced shoot growth under As(III) stress conditions.
Project description:The alkaliphilic halotolerant bacterium Bacillus sp. N16-5 often faces salt stress in its natural habitats. One-color microarrays was used to investigate transcriptome expression profiles of Bacillus sp. N16-5 adaptation reactions to prolonged grown at different salinities (0%, 2%, 8% and 15% NaCl) and the initial reaction to suddenly alter salinity from 0% to 8% NaCl. Salt induced gene expression was measured when culture was grown on different salinities (0%, 2%, 8% and 15% NaCl) to mid-logarithmic phase. And salt induced gene expression was also measured at 0 min, 10 min, 30 min, 60min, 120min after a sudden change salinity from 0% to 8% NaCl.
Project description:Alkaline hemicellulytic bacteria Bacillus sp. N16-5 has abroad substrate spectrum and exhibits great growth ability on complex carbohydrates. In order to get insight into its carbohydrate utilization mechanism, global transcriptional profiles were separately determined for growth on glucose, fructose, mannose, galactose, arabinose, xylose, galactomannan, xylan, pectin and carboxymethyl cellulose by using one-color microarrays.
