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New insights into the cis-regulation of the CFTR gene in pancreatic cells [ATAC-seq]

ABSTRACT: New insights into the cis-regulation of the CFTR gene in pancreatic cells [ATAC-seq]

PROVIDER: PRJNA1198966 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31736537_1.fastq.gz	Fastqsanger.gz
	SRR31736537_2.fastq.gz	Fastqsanger.gz
	SRR31736538_1.fastq.gz	Fastqsanger.gz
	SRR31736538_2.fastq.gz	Fastqsanger.gz
	SRR31736539_1.fastq.gz	Fastqsanger.gz

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New insights into the cis-regulation of the CFTR gene in pancreatic cells [CUT&Run]

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Project description:Loss of CFTR function in the pancreatic duct leads to dysregulated luminal pH causing premature activation of digestive enzymes and tissue necrosis. Drastic alterations in pancreatic tissue architecture and cellular composition changes the microenvironment of the islets. Given that CFTR is expressed in the pancreatic ducts, we hypothesized that loss of functional CFTR impacts islet function by modifying the ductal secretome. To this end, we developed a long-term in vitro pancreatic duct epithelial cell culture system and polarized both WT and CFTR-KO (CF) ferret duct epithelial cells. We profiled the apical and basolateral secretome, and the cellular proteome of both WT and CF duct epithelium using quantitative mass spectrometry. Bioinformatic analysis of differentially secreted proteins mapped to their cognate receptors provided a list of putative paracrine interactions that affect islet function. Signaling pathways and upstream regulators that alter the secretome and cellular proteome profile were computationally mined to characterize disease causing mechanisms. In this study, we provide a proteomic roadmap of perturbed autocrine and paracrine signals from the CF pancreatic duct.

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