Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1199219 | ENA |
REPOSITORIES: ENA
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Project description:The aim of this study was to explore whether, and if so, how Bacillus subtilis KC1 can enhance the growth performance of broilers that have been adversely affected by Mycoplasma gallisepticum (MG) infection. A total of 96 1-day-old male broilers were randomly divided into 4 groups: the control group (basal diet), the MG group (basal diet + MG challenge), the Bacillus subtilis KC1 group (basal diet + Bacillus subtilis KC1 supplementation), the Bacillus subtilis KC1 + MG group (basal diet + Bacillus subtilis KC1 supplementation + MG challenge). The trial lasted 42 days, and the results showed that the MG group had significantly reduced body weight and average daily gain, as well as increased feed conversion ratio of broilers, compared to the control group. Dietary supplementation with Bacillus subtilis KC1 significantly improved the growth performance of MG-infected broilers. In addition, dietary supplementation with Bacillus subtilis KC1 significantly improved oxidative stress and inflammatory response markers, characterized by increased superoxide dismutase levels and reduced levels of malondialdehyde, interleukin-1β, and tumor necrosis factor-α. Furthermore, both metabolomics and transcriptomics analyses indicated that MG infection markedly disrupted amino acid metabolism in broilers, whereas Bacillus subtilis KC1 supplementation alleviated the abnormal amino acid metabolism caused by MG infection. These results suggested that Bacillus subtilis KC1 may alleviate the poor growth performance caused by MG infection in broilers by improving amino acid metabolism.
2024-10-23 | GSE273913 | GEO
Project description:RNA N6-melthyladenosine has been suggested to play important roles in various biological processes. Chicken ovary development is a process controlled by complex gene regulations. In this study, transcriptome-wide m6A methylation of the Wuhua yellow-feathered chicken ovaries before and after sexual maturation was profiled to identify potential molecular mechanisms underlying chicken ovary development. The results showed that m6A levels of mRNAs changed dramatically during sexual maturity. A total of 1476 differential m6A peaks were found between these two stages with 662 significantly up-regulated methylation peaks and 814 down-regulated methylation peaks after sexual maturation. A positive correlation was found between the m6A peaks and gene expression levels. Functional enrichment analysis indicated that apoptosis related pathways might be the key molecular regulatory pathway underlying the poor reproductive performance of Wuhua yellow-feathered chicken. The fine expressional regulation of genes related to follicles development and follicle atresia controlled by m6A during the maturity results in the poor reproductive performance in the Wuhua yellow-feathered chicken. However, the regulatory mechanisms are still unclear, thus more further studies are required. The pathways and corresponding candidate genes found here may be useful for molecular design breeding for improving egg production performance in Chinese local chicken breed, and it will also benefit for the genetic resource protection of valuable avian species.
2023-11-01 | GSE239644 | GEO
Project description:The multiple toxic effects of Ochratoxin A(OTA) are a real threat for human beings and animal health. It has been suggested that probiotic improve the epithelial barrier disruption and reduce cell damage induced by mycotoxins. However, the exact effect and mechanism of probiotic Bacillus subtilis CW14 on epithelial barrier function is not well understood. The aim of this study was to examine whether B. subtilis CW14 could protect against OTA-induced barrier disruption and cell damage in human intestinal epithelial cell line (Caco-2). The results showed that OTA was absorbed efficiently in Caco-2 cells, led to microvilli disruption and tight junction protein(ZO-1 and claudin-1) damage, and suppressed cell proliferation by arresting cell cycle in G2/M phase and promoting apoptosis. The addictive of B. subtilis CW14 strains could partially reversed the tight junction injury by improving the ZO-1 protein expression and reduced the apoptosis induced by OTA. Furthermore, the transcriptome analysis data indicated that OTA mainly down-regulated the gene expression involved in tight junctions, cell cycle, and apoptosis-related signaling pathways. While the B. subtilis CW14 has the potential to protect epithelial barrier through activating the toll-like receptor signaling pathway, and could partially repair the OTA damage by down-regulating the TRAIL death receptor genes and up-regulating the DNA repair genes. Our findings suggest an important implication for the role of B. subtilis CW14 on the regulation of tight junction proteins and reduction cell death in intestine epithelial cells, and that is a potential candidate as a food additive to protect against intestinal damage.
2019-12-31 | GSE115081 | GEO