Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1199219 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The aim of this study was to explore whether, and if so, how Bacillus subtilis KC1 can enhance the growth performance of broilers that have been adversely affected by Mycoplasma gallisepticum (MG) infection. A total of 96 1-day-old male broilers were randomly divided into 4 groups: the control group (basal diet), the MG group (basal diet + MG challenge), the Bacillus subtilis KC1 group (basal diet + Bacillus subtilis KC1 supplementation), the Bacillus subtilis KC1 + MG group (basal diet + Bacillus subtilis KC1 supplementation + MG challenge). The trial lasted 42 days, and the results showed that the MG group had significantly reduced body weight and average daily gain, as well as increased feed conversion ratio of broilers, compared to the control group. Dietary supplementation with Bacillus subtilis KC1 significantly improved the growth performance of MG-infected broilers. In addition, dietary supplementation with Bacillus subtilis KC1 significantly improved oxidative stress and inflammatory response markers, characterized by increased superoxide dismutase levels and reduced levels of malondialdehyde, interleukin-1β, and tumor necrosis factor-α. Furthermore, both metabolomics and transcriptomics analyses indicated that MG infection markedly disrupted amino acid metabolism in broilers, whereas Bacillus subtilis KC1 supplementation alleviated the abnormal amino acid metabolism caused by MG infection. These results suggested that Bacillus subtilis KC1 may alleviate the poor growth performance caused by MG infection in broilers by improving amino acid metabolism.
2024-10-23 | GSE273913 | GEO
Project description:RNA N6-melthyladenosine has been suggested to play important roles in various biological processes. Chicken ovary development is a process controlled by complex gene regulations. In this study, transcriptome-wide m6A methylation of the Wuhua yellow-feathered chicken ovaries before and after sexual maturation was profiled to identify potential molecular mechanisms underlying chicken ovary development. The results showed that m6A levels of mRNAs changed dramatically during sexual maturity. A total of 1476 differential m6A peaks were found between these two stages with 662 significantly up-regulated methylation peaks and 814 down-regulated methylation peaks after sexual maturation. A positive correlation was found between the m6A peaks and gene expression levels. Functional enrichment analysis indicated that apoptosis related pathways might be the key molecular regulatory pathway underlying the poor reproductive performance of Wuhua yellow-feathered chicken. The fine expressional regulation of genes related to follicles development and follicle atresia controlled by m6A during the maturity results in the poor reproductive performance in the Wuhua yellow-feathered chicken. However, the regulatory mechanisms are still unclear, thus more further studies are required. The pathways and corresponding candidate genes found here may be useful for molecular design breeding for improving egg production performance in Chinese local chicken breed, and it will also benefit for the genetic resource protection of valuable avian species.
2023-11-01 | GSE239644 | GEO
Project description:<p>This study aimed to investigate the effects of wet-fermented brewer's grains (WFBG) on gut development and serum metabolism in white-feathered broilers. A total of 192 one-day-old male broilers (initial body weight: 36.46 ± 0.93 g) were randomly assigned to two treatment groups: the control group (0% WFBG) and the experimental group (20% WFBG inclusion), with six replicates of 16 birds per replicate. The results of intestinal morphological parameters quantified using ImageJ software after hematoxylin-eosin (HE) staining showed that compared with the control group, broilers fed a diet supplemented with 20% WFBG had significantly improved duodenal development. Specifically, the duodenal villus height (VH) increased by 10.2% (P < 0.05), and the villus height-to-crypt depth ratio (VH/CD) increased by 27.2% (P < 0.05)—both indicators reflecting the enhanced duodenal development. Through untargeted metabolomics analysis for screening differentially-expressed metabolites (DEMs) of serum samples, 211 DEMs were identified, including 98 upregulated DEMs and 113 downregulated DEMs in the WFBG group. KEGG pathway enrichment analysis revealed that these DEMs were significantly associated with key metabolic processes, including linoleic metabolic pathways, linoleic acid metabolism, phenylalanine metabolism, and other relevant pathways. Specifically, key DEMs involved in amino acid metabolism showed significantly increased phenylalanine levels and decreased 4-HPA and 3-HPA levels (P < 0.05). In conclusion, the inclusion of 20% WFBG in the diet of white-feathered broilers significantly promoted intestinal development. These favorable outcomes tie to modified serum metabolic profiles and adjustments to lipid/amino acid metabolism in broilers, underscoring WFBG’s significance for boosting broiler intestinal development while also adding to the theoretical framework for poultry by-product-based feeds. </p>
2025-09-30 | MTBLS13078 | MetaboLights