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Pinctada maxima breed:silver-lipped pearl oyster

ABSTRACT: Pinctada maxima breed:silver-lipped pearl oyster Genome sequencing

PROVIDER: PRJNA1199952 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR31787121_subreads.fastq.gz	Fastqsanger.gz
	SRR31789460_1.fastq.gz	Fastqsanger.gz
	SRR31789460_2.fastq.gz	Fastqsanger.gz
	SRR31790697_1.fastq.gz	Fastqsanger.gz

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Global gene expression profile for pearl oyster

Project description:This SuperSeries is composed of the following subset Series: GSE13980: Analysis of the global gene expression profile for pearl oyster, Pinctada maxima, exposed to organotin (tributyltin) GSE14303: Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima GSE14305: The microstructural, mineralogical and transcriptional developments of shell biomineralization of Pinctada maxima Refer to individual Series

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Proteomic characterisation of byssus components in the pearl oyster, Pinctada maxima

Project description:Many bivalve species produce groups of strong proteinaceous byssal threads to rigidly attach to underwater substrates. Fibres like these have potential applications as biomedical materials due to their unique mechanical characteristics. The byssus and byssal thread producing glands of Pinctada maxima have not yet been characterised. RNA was isolated from P. maxima foot and byssal stem region tissues and sequenced using the Illumina platform. A de novo reference transcriptome comprising 34,281 contiguous sequences was assembled, and tissue replicates were mapped against the reference for quantitative analysis. Tryptic digests of byssal threads were analysed by LC-MS/MS. The resultant peptides were matched to 62 protein sequences derived from our reference transcriptome. Components of the byssus were identified for further characterisation, including a highly expressed perlucin-like foot protein (Pmfp1) and a recently identified protein that we refer to herein as glycine-rich thread (GRT) protein. This work provides principal knowledge on the molecular components of the byssus for P. maxima and the foot ultrastructure involved in the creation of byssal threads. This study advances our knowledge of byssus biosynthesis in non-mytilids, providing a platform for the design of new marine biopolymers.

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Project description:To elucidate the modulatory participation of miRNAs in mollusk biomineralization, we have employed high-throughput sequencing to identify miRNAs of pearl oyster, Pinctada fucata. Our study focused on the miRNA expression profile of the mantle, an organ responsible for shell formation of the oyster. The pearl oysters were cultured in the tank with the maintaining conditions of temperature 19 ℃, PH 8.1 and salinity 33‰ in recirculating seawater.

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Project description:Silver-lipped pearl oyster transcriptome project

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Comparative proteomics reveals the immune reaction of the pearl oyster Pinctada fucata serum to xenograft from Pinctada maxima, the pearl oyster, allograft transplantation, xenograft transplantation, Immune rejection, comparative proteomics, pearl

Project description:To determine the mechanism underlying the immune response after allograft (mantle grafts of Pinctada fucata) and xenograft (mantle pieces of Pinctada maxima) transplantations in the pearl oyster Pinctada fucata, two sets of serum were obtained at different times (6, 12, 24, 48, 96, 144 and 192 h) after allograft and xenograft transplantations and proteomic responses were evaluated by using isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography (LC) / tandem mass spectrometric (MS) analysis.

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