Project description:Wastewater treatment plants (WWTPs) and Drinking water treatment plants (DWTPs) are critical points for public health for persistently remaining microorganisms after treatment may pose a risk. This study aimed to conduct microbial metagenomic analyses on waters from both DWTPs and WWTPs under the Istanbul Water and Sewerage Administration (ISKI). In this study a total of 52 samples were included, comprising 18 samples from DWTPs and 34 from WWTPs. All water samples underwent pre-isolation filtration. DNA isolation was conducted using filter material, followed by library preparation and sequencing on a NovaSeq 6000 instrument following the manufacturer's guidelines.
Project description:Sorghum has been widely used for liquor production and brewing, but how to make efficiently utilize sorghum straw (SS) has become an urgent problem. Meanwhile, the wastewater produced by winemaking is typical organic wastewater with a high ammonium concentration. To solve the problem of resource utilization of SS and remove ammonium from water, SS was used to prepare biochar as an adsorbent for ammonium adsorption. Batch adsorption experiments were carried out to study the influencing factors and adsorption mechanisms of ammonium onto sorghum straw biochar (SSB). The results showed that the adsorption capacity of SSB was much higher than that of SS. The SSB pyrolyzed at 300 °C had the highest adsorption capacity. The favorable pH was 6-10, and the optimal dosage was 2.5 g/L. The adsorption process and behavior conformed to the pseudo-second-order kinetic and Langmuir isotherm adsorption models. The maximum ammonium adsorption capacity of SSB at 45 °C was 7.09 mg/g, which was equivalent to 7.60 times of SS. The ammonium adsorption of SS and SSB was mainly chemical adsorption. The regeneration test indicated that SSB had good regeneration performance after three adsorption-regeneration cycles. This work suggests that SSB could be potentially applied to sewage treatment containing ammonium to achieve the purpose of resource recycling.
Project description:Salinized soil often exhibits high salt content and low nutrient availability, leading to the reduction of soil ecosystem function and crop productivity. Although straw return has profound effects on saline soil improvement, how soil quality index (SQI), soil ecosystem multifunctionality (EMF), and crop yield respond to different organic ameliorants remain unclear. Herein, a field experiment was established to explore the influence of various straw management strategies (no organic ameliorant, CK; corn straw return, CS; and corn straw biochar return; CB) on the saline soil functions and crop productivity. In relation to CK and CB, CS significantly improved SQI by 52% and 35%, respectively. This may be due to the decreased soil salt (especially soluble Na+) and increased available nutrients under corn straw return. Furthermore, CS increased soil EMF than CK by 71% and CB by 39%, which was caused by the increased activities of 1,4-β-glucosidase, β-1,4-N-acetyl-glucosaminidase, and leucine aminopeptidase. The linear model further supported that soil enzyme activities are positively related to available nutrient contents and negatively correlated with salt content. Moreover, the crop yield under CS significantly increased by 22% compared to CK. Also, soil quality positively influenced crop yield, with soil salt and available phosphorus being the primary influencing factors. However, crop yield was not sensitive to soil EMF. In summary, straw return was more beneficial to improving soil quality and crop productivity than biochar in the short term in saline soils.
Project description:The seasonal out-of-limit of manganese ions (Mn2+) in the drinking water reservoirs is an intractable problem to water supply, which can pose a threat to the human health. In this study, the removal of Mn2+ by using pristine (BC), pre-alkali (Pre-BC) and post-alkali (Post-BC) modified biochar originating from rice straw was investigated. The maximum adsorption capacities obtained for BC, Pre-BC, and Post-BC were 20.59, 28.37, and 8.06 mg g-1, respectively. The Langmuir isotherm model and the pseudo-second-order kinetic model were suitable fitting models to describe the adsorption process. The investigation of adsorption functions was carried out that revealed that the predominant forces were precipitation and cation exchange with the proportions of 43.38-69.15% and 38.05-55.79%, respectively. With regard to precipitation, Mn(ii) particles (Al-Si-O-Mn and MnCO3) and insignificantly oxidized insoluble Mn(iv) particles (MnO2) were formed on the biochar surface. Alkali and alkaline earth metals facilitated the behavior of cation exchange, where the primary contributing ions for cation exchange were Na+, Mg2+ and Ca2+ during the adsorption process. These outcomes suggest that alkali pre-treated modification of biochar is practical for the application of manganese pollution control in lakes and reservoirs.
Project description:Nano-sized biochar (NBC) has received increasing attention due to its unique physicochemical characteristics and environmental behaviour, but an understanding of its redox properties is limited. Herein, the redox properties of NBC derived from wheat straw were investigated at two pyrolysis temperatures (400 and 700 °C). These NBC materials were prepared from bulk-biochar by grinding, ultrasonication and separation treatments. The resulting NBC had average particle sizes of 78.8 ± 1.9 and 122.0 ± 2.1 nm after 400 and 700 °C treatments, respectively. The physicochemical measurements demonstrated that both the NBC prepared at 400 °C (NBC-400) and the NBC prepared at 700 °C (NBC-700) were enriched in carboxyl and phenolic oxygen-content groups. Electrochemical analyses showed that both NBC-400 and NBC-700 were redox active and had an electron transfer capacity (ETC) of 196.57 μmol-1 gC -1 and 363.47 μmol-1 gC -1, respectively. On the basis of its redox activity of NBC, the NBC was capable of mediating the reduction of iron and manganese minerals as well as the degradation of methyl orange (MO) by sulfide. The NBC-700 could stimulate these reactions better than the NBC-400 due to its higher redox activity. Meanwhile, the NBC was more active in stimulating these reactions than bulk-biochar. Our results highlight the importance of size in evaluating the redox reactivity of biochar and related environmental processes and improve our understanding of the redox properties of biochar.
Project description:Cadmium contamination in waters and soils can lead to food chain accumulation and ultimately deterioration in human health; means for reducing bioavailable Cd are desperately required, and biochars may play a role. Long-term (240 d) lab incubation experiments were utilized to explain wheat straw-derived biochar effects on Cd sorption and decreasing Cd bioavailability in soils and solutions (0, 5, and 15% biochar as wt:wt or wt:vol, respectively), and to identify Cd forms present using both the European Community Bureau of Reference (BCR) chemical sequential extraction procedure and synchrotron-based X-ray absorption spectroscopy (XAS). Biochar Cd removal was up to ~90% from Cd-containing solutions and contaminated soil as compared to the control. Based on the wet chemical sequential extraction procedure in conjunction with XAS, biochar application promoted the formation of (oxy)hydroxide, carbonate, and organically bound Cd phases. As a material, biochar may be promoted as a tool for reducing and removing bioavailable Cd from contaminated waters and soils. Thus, biochar may play a role in reducing Cd bioaccumulation, trophic transfer, and improving environmental quality and human health.
Project description:The utilization of high-efficiency adsorption materials to reduce cadmium pollution in aquatic environments is the focus of current environmental remediation research. Straw waste and sludge, which are available in huge amounts, can be best utilized in the preparation of environmental remediation materials. In this study, six types of biochar (SBC, CBC, DBC, SD1BC, SRDBC, and SCDBC) were prepared from straw and sludge by co-pyrolysis, and their cadmium adsorption mechanisms were explored. Cd(II) adsorption isotherms and kinetics on the biochar were determined and fitted to different models. Kinetic modeling was used to characterize the Cd(II) adsorption of biochar, and findings revealed the process of sorption followed pseudo-second-order kinetics (R2 > 0.96). The Langmuir model accurately represented the isotherms of adsorption, indicating that the process was monolayer and controlled by chemical adsorption. SCDBC had the highest capacity for Cd(II) adsorption (72.2 mg g-1), 1.5 times greater than that of sludge biochar, and 3 times greater than that of corn straw biochar. As the pH level rose within the range of pH 5.0 to 7.0 and the ionic strength decreased, the adsorption capacity experienced an increase. SCDBC contained CaCO3 mineral crystals before Cd(II) adsorption, and CdCO3 was found in SCDBC after adsorbing Cd(II) via X-ray diffraction analysis; the peak of Cd could be observed by Fourier transform infrared spectroscopy after the adsorption of Cd(II). The possible adsorption of Cd(II) by SCDBC occurred primarily via surface complexation with active sorption sites, precipitation with inorganic anions, and coordination with π electrons. Collectively, the study suggested that the six types of biochar, particularly SCDBC, could be used as highly efficient adsorbents for Cd(II) removal from aquatic environments.
Project description:We present metaproteome data from wheat rhizosphere from saline and non-saline soil. For collection and acquisition of metaproteome from wheat rhizosphere under saline and normal conditions, a survey was conducted in regions of Haryana, India. Samples from 65 days old plants (wheat var HD2967) were collected and pooled and based on EC,saline (NBAIM B; EC 6mS cm-1; pH 9.0; Bhaupur 2, Haryana, INDIA; 29°19'8"N;76°48'53"E) and normal soil samples (NBAIM C; EC 200 uS cm-1; pH 7.2; Near Nainform, Haryana, INDIA; 29°19'8"N;76°48'53"E) were selected for isolation of proteome with the standardized protocol at our laboratory followed by metaproteome analysis with the standardized pipepline. In total 1538 and 891 proteins were obtained from wheat rhizosphere from saline and non-saline respectively with the given parameters and software. Among 1410 proteins unique for saline soil, proteins responsible for glycine, serine and threonine metabolism and arginine and proline biosynthesis were found in saline and absent in non-saline. The present study extends knowledge about the physiology and adaptations of the wheat rhizosphere associated microbiota under saline soil.
Project description:Straw return is an effective method for disposing agricultural residues. It not only utilizes agricultural waste but also improves soil. In the current review, different crop straw and its characteristics were highlighted, and patterns of straw return were explored (including straw return, straw biochar return, and their combined with fertilizer return), as well as their environmental impacts were outlined. In addition, the effects of straw return and straw biochar amendment on soil properties [e.g., pH, soil organic carbon (SOC), soil nitrogen (N)/phosphorus (P)/potassium (K), soil enzyme activities, and soil microbes] were discussed. Information collected from this review proposed that straw return and straw biochar return or in combination with fertilizer is an applicable way for improving soil fertility and enhancing crop production. Straw return is beneficial to soil physicochemical properties and soil microbial features. The rice straw has positive impacts on crop growth. However, there are different climate types, soil types and crops in China, meaning that the future research need long-term experiment to assess the complex interactions among straw, soil, and plant eco-systems. Accordingly, this review aims to provide available information on the application of straw return in terms of different patterns of its to justify and to expand their effective promotion.
Project description:Microbial decomposition plays a crucial role in the incorporation of straw and straw biochar (SSB) into soil. Lime concretion black soil (LCBS) is a typical low-medium crop yield soil, and it is also one of the main soil types for grain production in China. However, the link between SSB additions and soil bacterial communities in LCBS remains unclear. This study explored the effects of SSB incorporation on bacterial community composition, structure and co-occurrence network patterns at different soil depths and maize growth stages. The results showed that soil PH, soil organic matter and total nitrogen significantly affected the seasonality and stratification of the soil bacterial community. The composition and diversity of bacterial communities were significantly affected by growth period and treatment rather than soil depth. Specifically, the bacterial community diversity increased significantly with crop growth at 0-20 cm, decreased the relative abundance of Actinobacteria, and increased the relative abundance of Proteobacteria and Acidobacteria. SF (straw with fertilizer) and BF (straw biochar with fertilizer) treatments decreased bacterial community diversity. Co-occurrence networks are more complex in BF, S (straw), and SF treatments, and the number of edge network patterns is increased by 92.5, 40, and 60% at the maturity stage compared with F (fertilizer) treatment, respectively. Moreover, the positive effect of straw biochar on the bacterial network pattern increased with time, while the effect of straw weakened. Notably, we found that rare species inside keystone taxa (Gemmatimonadetes and Nitrospirae) play an indispensable role in maintaining bacterial network construction in LCBS. This study offers a comprehensive understanding of the response of soil bacterial communities to SSB addition in LCBS areas, and provides a reference for further improvement of LCBS productivity.