Project description:we report a transcriptome-wide comparative investigation between surface and cave species in Sinocyclocheilus. De novo transcriptome assemblies were performed on surface and cave species; then the Sinocyclocheilus contigs were annotated with Gene Ontology. RNA-Seq assays revealed reduced transcription of a series of visual phototransduction and retinal disease related genes in cave-dwelling species compared with surface species. Degeneration of the retina in Sinocyclocheilus cavefish might occur in a lens-independent way by the down-regulation of several transcriptional factors, which have direct roles in retina development and maintenance, such as crx, rorb and Wnt pathway members. Examination of 2 different eye samples in 2 Sinocyclocheilus species.
Project description:we report a transcriptome-wide comparative investigation between surface and cave species in Sinocyclocheilus. De novo transcriptome assemblies were performed on surface and cave species; then the Sinocyclocheilus contigs were annotated with Gene Ontology. RNA-Seq assays revealed reduced transcription of a series of visual phototransduction and retinal disease related genes in cave-dwelling species compared with surface species. Degeneration of the retina in Sinocyclocheilus cavefish might occur in a lens-independent way by the down-regulation of several transcriptional factors, which have direct roles in retina development and maintenance, such as crx, rorb and Wnt pathway members.
Project description:To facilitate new studies in the Amaryllidoideae subfamily, we present AmarylOmicBase, a unified transcriptomic database that integrates assemblies, annotations, and expression profiles from 39 studies, covering 29 species across 13 genera of Amaryllidoideae. The AmarylOmicBase includes both published and de novo assemblies generated from published raw data using Trinity or IsoSeq workflows and provides standardized functional annotation and quantitative expression datasets. Preprint - https://doi.org/10.1101/2025.11.24.690262
Project description:The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective fashion. Here, we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67X coverage, Sample GSM1551550). We then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors Aedes aegypti and Culex quinquefasciatus, each consisting of three scaffolds corresponding to the three chromosomes in each species. These assemblies indicate that virtually all genomic rearrangements among these species occur within, rather than between, chromosome arms. The genome assembly procedure we describe is fast, inexpensive, accurate, and can be applied to many species.
Project description:To facilitate new studies in the Amaryllidoideae subfamily, we present AmarylOmicBase, a unified transcriptomic database that integrates assemblies, annotations, and expression profiles from 39 studies, covering 27 Amaryllidoideae species and 4 hybrid cultivars across 13 genera of Amaryllidoideae. The AmarylOmicBase (v2, https://doi.org/10.5281/zenodo.20349158) includes de novo assemblies generated from published raw data using Trinity or IsoSeq workflows and provides standardized functional annotation (trinotate) and quantitative expression datasets. Preprint - https://doi.org/10.1101/2025.11.24.690262
Project description:To facilitate new studies in the Amaryllidoideae subfamily, we present AmarylOmicBase, a unified transcriptomic database that integrates assemblies, annotations, and expression profiles from 39 studies, covering 27 species and 4 hybrid cultivars across 13 genera of Amaryllidoideae. The AmarylOmicBase (v2, https://doi.org/10.5281/zenodo.20349158) consists of de novo assemblies generated from published raw data using Trinity or IsoSeq workflows and provides standardized functional annotation and quantitative expression datasets. Preprint - https://doi.org/10.1101/2025.11.24.690262
Project description:To facilitate new studies in the Amaryllidoideae subfamily, we present AmarylOmicBase, a unified transcriptomic database that integrates assemblies, annotations, and expression profiles from 39 studies, covering 27 species and 4 hybrid cultivars across 13 genera of Amaryllidoideae. The AmarylOmicBase (v2, https://doi.org/10.5281/zenodo.20349158) consists of de novo assemblies generated from published raw data using Trinity or IsoSeq workflows and provides standardized functional annotation and quantitative expression datasets. Preprint - https://doi.org/10.1101/2025.11.24.690262
Project description:To facilitate new studies in the Amaryllidoideae subfamily, we present AmarylOmicBase, a unified transcriptomic database that integrates assemblies, annotations, and expression profiles from 39 studies, covering 27 species and 4 hybrid cultivars across 13 genera of Amaryllidoideae. The AmarylOmicBase (v2, https://doi.org/10.5281/zenodo.20349158) consists of de novo assemblies generated from published raw data using Trinity or IsoSeq workflows and provides standardized functional annotation and quantitative expression datasets. Preprint - https://doi.org/10.1101/2025.11.24.690262