Gars
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1201891 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Lepisosteus oculatus (spotted gar) genome, fLepOcu1, haplotype 1
| PRJNA1106998 | ENA
Project description:Lepisosteus oculatus (spotted gar) genome, fLepOcu1, haplotype 2
| PRJNA1106999 | ENA
Project description:Comparison between [gar-] and [GAR+] samples of the W303 background grown in 2% glucose. Three separate [gar-] and [GAR+] cultures were grown to late exponential phase (OD600~0.8) prior to phenol-chloroform extraction. Biological replicates are [gar-] samples #1, 2, and 3 and [GAR+] samples #1, 2, and 3. [gar-] and [GAR+] sample sets were compared to determine transcriptional differences.
2009-07-01 | E-GEOD-12479 | biostudies-arrayexpress
Project description:Comparison between [gar-] and [GAR+] samples of the W303 background grown in 2% glucose.
2009-07-01 | GSE12479 | GEO
Project description:Transcription and pre-mRNA splicing are coupled to promote gene expression and regulation. However, mechanisms by which transcription and splicing influence each other are still under investigation. The ATPase Prp5p is required for pre-spliceosome assembly and splicing proofreading at the branch-point region. From an open UV mutagenesis screen for genetic suppressors of prp5 defects and subsequent targeted testing, we identify components of the TBP-binding module of the Spt–Ada–Gcn5 Acetyltransferase (SAGA) complex, Spt8p and Spt3p. Spt8Δ and spt3Δ rescue the cold-sensitivity of prp5-GAR allele, and prp5 mutants restore growth of spt8Δ and spt3Δ strains on 6-azauracil. By chromatin immunoprecipitation (ChIP), we find that prp5 alleles decrease recruitment of RNA polymerase II (Pol II) to an intron-containing gene, which is rescued by spt8Δ. Further ChIP-seq reveals that global effects on Pol II-binding is mutually rescued by prp5 mutant and spt8Δ. Inhibited splicing caused by prp5-GAR is also restored by spt8Δ. In vitro assay indicates that Prp5p directly interacts with Spt8p, but not Spt3p. We demonstrate that Prp5p’s splicing proofreading is modulated by Spt8p and Spt3p. Therefore, this study reveals that interactions between the TBP-binding module of SAGA and the spliceosomal ATPase Prp5p mediate a balance between transcription initiation/elongation and pre-spliceosome assembly.
2020-04-17 | GSE147179 | GEO
Project description:We used microarrays to observe the global gene expression in hematopoietic stem and projenitor cells during ex vivo culture with DMSO (Blank) or with Garcinol (GAR) and identified distinct classes of up or down-regulated genes. CD34+ cells were cultured ex vivo with DMSO (Blank) or with Garcinol (GAR) for 7 days. CD34+ CD38- cells were sorted from cultured cells (blank and GAR) with a FACS and total RNA were extracted from the sorted cells (blank and GAR), respectively. Blank represents a control sample.
2012-05-31 | E-GEOD-29459 | biostudies-arrayexpress