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MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

ABSTRACT: MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

PROVIDER: PRJNA120333 | ENA |

REPOSITORIES: ENA

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MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

Project description:This SuperSeries is composed of the following subset Series: GSE19354: Expression data for rno-miR-146a overexpressing cell line HSC-2 GSE19462: microRNA profiling in vitro activated primary hepatic stellate cells Refer to individual Series

2010-05-18 | E-GEOD-19463 | biostudies-arrayexpress

MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

Project description:This SuperSeries is composed of the SubSeries listed below.

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Expression data for rno-miR-146a overexpressing cell line HSC-2

Project description:HSC-2 (hepatic stellate cells line from rat) were stably transfected with rno-miR-146a. Three different clones were selected (S1, S4, S5). We used Affymetrix rat genome RAT230 2.0 chip to monitor global transcriptome changes.

2009-12-08 | GSE19354 | GEO

Expression data for rno-miR-146a overexpressing cell line HSC-2

2009-12-07 | E-GEOD-19354 | biostudies-arrayexpress

Hepatocyte-derived exosomal miR-146a-5p inhibits hepatic stellate cell EMT process: a crosstalk between hepatocytes and hepatic stellate cells.

Project description: Not available

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miR-146a regulates many lncRNAs in RAS induced senescence [Anti-146a]

Project description:miR-146a is a known anti-inflammatory miRNA. Intringuigly, it is overexpressed in RAS-induced senescent cells which is accompanied with a rich pro-inflammatoy secretpry phenotype. We aim to study possible sponges for miR-146a.

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miR-146a Pulldown-sequencing data

Project description:miR-146a Pulldown-sequencing data

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Transcription profiling by array of human Jurkat T cells either over-expressing miR-146a or with miR-146a knockdown

Project description:Screening the differetial expressed genes with Jurkat-146a(miR-146a over expression),Jurkat-sponge(miR-146a knockdown),Jurkat-FF3(vector control)

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miR-146a regulates many lncRNAs in RAS induced senescence [miR-CLIP]

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miR-146a Pulldown-sequencing data

Project description:Human CD4+ Total memory cells (CD4+CD25-CD45RA-CCR7+/-) were activated with anti-CD3/anti-CD28 antibodies and transfected with biotinylated miR-146a mimic or biotinylated mimic negative control by Exiqon. After 24h cells were lysed and miRNA:mRNA complexes pulled-down with streptavidin agarose O/N at 4°C. RNA was then purified using TRI-reagent and RNA zymospin columns. Four independent donors were used for this experiment. Total RNA was sequenced in 2 lanes, paired ends. This experiment is assessing direct targets bound by miR-146a in human T cells.

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