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MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

ABSTRACT: MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

PROVIDER: PRJNA120333 | ENA |

REPOSITORIES: ENA

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MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

Project description:This SuperSeries is composed of the following subset Series: GSE19354: Expression data for rno-miR-146a overexpressing cell line HSC-2 GSE19462: microRNA profiling in vitro activated primary hepatic stellate cells Refer to individual Series

2010-05-18 | E-GEOD-19463 | biostudies-arrayexpress

MicroRNA studies in in vitro and in vivo activated rat hepatic stellate cells: Emphasis on miR-146a

Project description:This SuperSeries is composed of the SubSeries listed below.

2009-12-15 | GSE19463 | GEO

Expression data for rno-miR-146a overexpressing cell line HSC-2

Project description:HSC-2 (hepatic stellate cells line from rat) were stably transfected with rno-miR-146a. Three different clones were selected (S1, S4, S5). We used Affymetrix rat genome RAT230 2.0 chip to monitor global transcriptome changes.

2009-12-08 | GSE19354 | GEO

Expression data for rno-miR-146a overexpressing cell line HSC-2

2009-12-07 | E-GEOD-19354 | biostudies-arrayexpress

Hepatocyte-derived exosomal miR-146a-5p inhibits hepatic stellate cell EMT process: a crosstalk between hepatocytes and hepatic stellate cells.

Project description:Recently, Salidroside (Sal) has been demonstrated to suppress hepatic stellate cell (HSC) activation, a crucial event for liver fibrosis. Moreover, Sal has been reported to decrease hepatocyte injury. A growing number of reports have indicated that the crosstalk between hepatocytes and HSCs is very crucial for liver fibrosis development. Whether Sal-treated hepatocytes could inhibit HSC activation is unclear. Exosomes, as vital vehicles of intercellular communication, have been shown to transfer cargos between hepatocytes and HSCs. Herein, we aimed to investigate the roles of exosomal miRNAs from Sal-treated hepatocytes in HSC activation as well as liver fibrosis. Our results showed that Sal suppressed carbon tetrachloride (CCl4)-induced liver fibrosis in vivo. HSC activation as well as cell proliferation was repressed in HSCs co-cultured with Sal-treated hepatocytes. Interestingly, miR-146a-5p was up-regulated by Sal in CCl4-treated mice. Also, enhanced miR-146a-5p was found in hepatocytes isolated from Sal-treated CCl4 mice and hepatocyte-derived exosomes. Notably, hepatocyte exosomal miR-146a-5p contributed to HSC inactivation. Inhibiting miR-146a-5p in hepatocyte exosomes resulted in reduced E-cadherin (E-cad) and increased desmin in HSCs, indicating that miR-146a-5p caused HSC inactivation via epithelial-mesenchymal transition (EMT). miR-146a-5p inhibition-mediated HSC activation and EMT process were blocked down by loss of EIF5A2. Further studies revealed that EIF5A2 was a target of miR-146a-5p. Furthermore, exosomes with miR-146a-5p overexpression inhibited liver fibrosis in CCl4 mice. Collectively, exosomal miR-146a-5p from Sal-treated hepatocytes inhibits HSC activation and liver fibrosis, at least in part, by suppressing EIF5A2 and EMT process.

| S-EPMC10439924 | biostudies-literature

miR-146a regulates many lncRNAs in RAS induced senescence [Anti-146a]

Project description:miR-146a is a known anti-inflammatory miRNA. Intringuigly, it is overexpressed in RAS-induced senescent cells which is accompanied with a rich pro-inflammatoy secretpry phenotype. We aim to study possible sponges for miR-146a.

2021-05-11 | GSE150325 | GEO

miR-146a Pulldown-sequencing data

Project description:miR-146a Pulldown-sequencing data

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Transcription profiling by array of human Jurkat T cells either over-expressing miR-146a or with miR-146a knockdown

Project description:Screening the differetial expressed genes with Jurkat-146a(miR-146a over expression),Jurkat-sponge(miR-146a knockdown),Jurkat-FF3(vector control)

2010-03-24 | E-MEXP-2627 | biostudies-arrayexpress

miR-146a target identification in gastric cancer

Project description:We have found expression of miR-146a up-regulated in gastric cancer. To identify new targets of miR-146a we profiled the transcriptome after miR-146a over-expression in the human gastric cancer cell line SNU638. SNU638 cells were transfected in triplicates with 50 nM miR-146a or control (siGlo) using Lipofectamine 2000. Total RNA was harvested 24 h after transfection using Trizol reagent. There are a total of six arrays included in this experiment, including three biological replicates of mRNA expression after miR-146a over-expression and three controls in SNU638 cells.

2012-07-01 | E-MEXP-3479 | biostudies-arrayexpress

miR-146a regulates many lncRNAs in RAS induced senescence [miR-CLIP]

2021-05-11 | GSE150324 | GEO