Project description:While cold stress has been shown to seriously impact cattle industry, there are only a few reports investigating the effect of cold stress on cattle. Whether severe cold stress results in alterations in gene expression and affects molecular genetic mechanisms remains unknown. We used microarrays to analyze the alterations in gene expression in peripheral blood samples in response to cold exposure and identified differentially regulated genes.
Project description:We identified and functionally explored D. silvarum miRNAs involved in cold response to gain further understanding of the molecular regulatory mechanisms underlying the cold stress in ticks. The microRNA libraries of D. silvarum were established via high-throughput sequencing after exposure to different cold treatments. A total of 147 miRNAs, including 44 known miRNAs and 103 new miRNAs, were identified.
2021-09-29 | GSE184617 | GEO
Project description:Full-length transcriptome sequencing of Trifolium ambiguum Bieb
Project description:The biochemical and molecular mechanisms of insect cold acclimation prior to cold stress have been relatively well explored. The mechanisms linked to recovery and repair after cold stress received much less attention.Here we focus on recovery from cold stress in the larvae of vinegar fly (Drosophila melanogaster) that were exposed to two physiologically distinct cold stress situations; supercooling (S, survival > 90%) and freezing (F, survival < 10%), both at -5°C.The transcriptomic responses to cold stress were analyzed.
Project description:While cold stress has been shown to seriously impact cattle industry, there are only a few reports investigating the effect of cold stress on cattle. Whether severe cold stress results in alterations in gene expression and affects molecular genetic mechanisms remains unknown. We used microarrays to analyze the alterations in gene expression in peripheral blood samples in response to cold exposure and identified differentially regulated genes. This study was performed on 30 healthy Sanhe heifers with similar genetic backgrounds, weight, and age. In order to induce cold stress, the cattle were transferred outdoor and were exposed to a temperature of -32°C for 3 hours followed by housing in cowshed at 5°C for 15 hours. Blood samples with EDTA were collected from each animal before and after the cold exposure. After total RNA was isolated from blood cells, six RNA samples (three derived before and three after the cold exposure), were collected from three animals randomly selected from the 30 healthy heifers for gene expression profiling in response to severe cold stress.
Project description:Jojoba (Simmondsia chinensis) is a new semi- arid, oil- producing industrial crop that has attracted much attention in recent years. Low temperature is one of the major environmental stress that impairs plant growth and development. To better understand the molecular mechanisms of cold stress adaptation and acclimation of jojoba plants, a quantitative proteomic analysis using iTRAQ technology was conducted to detect the effects of cold stress on protein expression profiles in jojoba seedlings. Our work provided useful infomation for understanding the cold stress response and cold acclimation in jojoba.
Project description:By comparing transcriptome profiles for differential gene expression, genes and pathways probably involved in insect cold hardinesswere identified. This dataset provides a global transcriptome data and differential transcriptome comparisons in E. hippophaecolus under cold stress. This may facilitate future genomic studies aimed at improving our understanding of the molecular mechanisms underlying the response of insects to low temperatures.
Project description:Basic region/leucine zipper (bZIP) transcription factors play vital roles in the abiotic stress response of plants. However, little is known about the function of bZIP genes in Camellia sinensis. Here, we show that CsbZIP6 is induced during cold acclimation in tea plant. Constitutive overexpression of CsbZIP6 in Arabidopsis lowered the plants’ tolerance to freezing stress and ABA exposure during seedling growth. Compared to wildtype (WT) plants, CsbZIP6 overexpression (OE) lines exhibited increased levels of electrolyte leakage (EL) and malondialdehyde (MDA) contents and reduced levels of total soluble sugars (TSS) under cold stress conditions. Microarray analysis of transgenic Arabidopsis revealed that many differentially expressed genes (DEGs) between OE lines and WT plants could be mapped to ‘response to cold’ and ‘response to water deprivation’ terms based on GO analysis. Interestingly, CsbZIP6 overexpression repressed most of the cold- and drought-responsive genes as well as the starch metabolism under cold stress conditions. Taken together, our data suggests that CsbZIP6 functions as a negative regulator of the cold stress response in Arabidopsis thaliana, potentially by down-regulating cold-responsive genes. To obtain insights into the molecular mechanisms by which CsbZIP6 mediates senstivity to cold stress in Arabidopsis plants, gene expression profiles in leaves of two CsbZIP6 OE lines and WT plants under normal (22ºC) and cold (4ºC) conditions were compared. The Agilent Arabidopsis Gene Expression (4×44K, Design ID: 021169) was used in this experiment.