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ABSTRACT: Near Infrared Laser Irradiation Reverses the Declining Quality of Post-Ovulatory Aging and Reproductive Aged Oocytes

PROVIDER: PRJNA1204317 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31853979_1.fastq.gz	Fastqsanger.gz
	SRR31853979_2.fastq.gz	Fastqsanger.gz
	SRR31853980_1.fastq.gz	Fastqsanger.gz
	SRR31853980_2.fastq.gz	Fastqsanger.gz
	SRR31853981_1.fastq.gz	Fastqsanger.gz

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Gene expression in a 3-dimensional reconstructed human corneal epithelial tissue after near-infrared irradiation simulating solar near-infrared radiation that reaches human tissues on the Earthâs surface

Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered broad-spectrum near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation. We performed 5 rounds of near-infrared irradiation (at 10 J âcm2) using 2 sets of transparent polycarbonate plates, one to block UV and the other to block both UV and near-infrared.

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Project description:Nicotinamide mononucleotide supplementation reverses the declining quality of maternally aged oocytes

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Project description:This study compares miRNA expression profiles in mouse oocytes as young oocytes vs aged oocytes, and growing oocytes vs small oocytes from primordial follicles. Oocytes were derived from the ovary of young (6-8 week-old) C57BL/6 mice and aged (41-43 week-old) mice and pooled according to whether they were 20 to 50 um or 60 to 80 um in diameter. Of oocytes with the diameter of more than 60um, oocyte from young mice are called ‘young oocytes’ and those from aged mice near the end of their reproductive life span are called ‘aged oocytes’ to analyze the miRNA profiling associated with aging. They were also each called ‘small oocytes’ or ‘large oocytes’ from the size of 60um so as to investigate miRNA profiling associated with growing. Total RNA from oocytes was isolated using mirVana miRNA Isolation Kit (Applied Biosystems). MiRNA expression was profiled using Agilent's Mouse miRNA Microarray Kit (G4472A) annotated against the Sanger miRBASE 10.1 database of miRNAs. This miRNA microarray was provided by Agilent Technologies (Santa Clara, CA). Each sample was run in duplicate.

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Project description:Advanced maternal age, defined as 35 years or older, is associated with a decline in both ovarian reserve and oocyte quality, which leads to the female infertility, pregnancy loss, fetal anomalies, stillbirth, and obstetric complications. At present, the effective approaches to counteract the maternal age-related decay of oocyte quality are still not fully determined. Here, we report that in vivo supplementation of nicotinamide mononucleotide (NMN) efficaciously ameliorates the quality of oocytes from naturally aged mice by recovering nicotinamide adenine dinucleotide (NAD + ) levels in oocytes. NMN supplementation increases the number of antral follicles, ovulated oocytes and matured oocytes from aged mice. Specifically, NMN supplementation maintains the normal spindle/chromosome structure and dynamics of cortical granule component ovastacin to ensure the meiotic competency and fertilization ability of aged oocytes. Moreover, single cell transcriptome analysis shows that the beneficial effect of NMN on the aged oocytes is mediated by the restoration of the mitochondrial function, thereby reducing the accumulated ROS to suppress the occurrence of apoptosis. To sum up, our data reveal that supplementation of NMN is a feasible approach to prevent oocyte quality from advanced maternal age-related deterioration, contributing to improve the reproductive outcome of aged women and the assisted reproductive technology.

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