Project description:Objectives: To identify gene expression changes in acne flare-up patients, thereby exploring the mechanisms of acne flare-up after treatment. Methods: 11 acne patients and 3 healthy people were divided into 4 groups (group1: 4 with flare-up, group2: 4 with improvement, group3: 3 without obvious changes, group4: healthy control). Peripheral blood of patients before and after isotretinoin or minocycline were collected. RNA-seq were used to detect the gene expression. We applied data in self-contrast and intergroup comparisons. Results: In the self-contrast of group1, 22 upregulated genes were involved in Toll-like receptor signaling pathway and inflammatory response. Comparing group1 and group3 before treatment, 1778 upregulated genes enriched in Th17 cell differentiation, while 57 downregulated genes enriched in defensive response to organism. Conclusions: The gene expression profiles of acne flare-up patients changed. Inflammatory, immune responses played a prominent role in acne flare-up process and relatively weak defensive response to microbes, comedogenesis might be risk factors.
Project description:Blood samples from healthy controls and PAD patients were collected at Xiangya Hospital, China. Two to three milliliters of venous blood were drawn into EDTA-coated tubes and stored at 4°C. Plasma was isolated after centrifugation (2,000 g, 10 min, 4°C), aliquoted, and send to perform MS. Group1: Health control D1, A5, A46, B14,B22; Group2: PAD C6 C29 D45 A32
Project description:The prevalence of glioblastoma increases with age. Advanced age is also one of the best-established risk factors for sporadic Alzheimer's disease (AD). Previous histopathological studies detected the occurrence of AD features in frontal cortex specimens from glioblastoma patients. This study aimed to identify AD-related transcript changes in cerebral cortex specimens obtained during surgery for glioblastoma. The study analyzed cerebral cortex specimens from three groups of glioblastoma patients (Group1, Group2, Group3). The transcriptome study of this Group1 includes specimens from 10 glioblastoma patients (Patient1-Patient10).
Project description:CS Baby Biome randomized control trial aims to investigate if the timing of intrapartum antibiotics given to mother influences the infant gut microbiome composition. The study was performed in women delivering via elective CS, who received antibiotics prior to skin incision, or after umbilical cord clamping.
