Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

ABSTRACT: Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

PROVIDER: PRJNA1206966 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31899600_1.fastq.gz	Fastqsanger.gz
	SRR31899600_2.fastq.gz	Fastqsanger.gz
	SRR31899601_1.fastq.gz	Fastqsanger.gz
	SRR31899601_2.fastq.gz	Fastqsanger.gz
	SRR31899602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

Project description:Anillin (ANLN), a mitotic protein that regulates contractile ring assembly, has been reported as an oncoprotein. However, the function of ANLN in cancer cells, especially in the nucleus, has not been fully understood. Here, we report a previously unknown role of nuclear ANLN in gene transcriptional regulation. We found that nuclear ANLN directly interacts with the RNA polymerase II (Pol II) large subunit. ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and cell differentiation. Our results reveal the function of nuclear ANLN in transcriptional regulation, providing a theoretical basis for ESCC treatment.

2025-01-11 | GSE285915 | GEO

RNA Polymerase II transcription independent of TBP (CUT&Tag)

Project description:Using CUT&Tag, a chromatin profiling technique, we show that TBP depletion via IAA surprisingly does not affect RNA Pol II transcription but affects RNA Pol III transcription. Additionally, induction of genes via heat shock and retinoic acid treatment does not require TBP. We also show that a metazoan specific paralog TRF2 does not compensate for TBP for RNA Pol II transcription and that the TFIID subunit of the Pre-initiation Complex can still form with specific subunits still binding onto DNA when TBP is depleted.

2023-04-12 | GSE206694 | GEO

Role of Setd2 in regulatory T cells [Pol II CUT&Tag]

Project description:Role of Setd2 in regulatory T cells [Pol II CUT&Tag]

| PRJNA758043 | ENA

HCT116_SMARCA4 CUT&Tag

Project description:HCT116_SMARCA4 CUT&Tag

| PRJNA875063 | ENA

CUT&TAG of OE19 cell line

Project description:To map the chromatin binding sites for SMC1 in OE19 cells, we performed CUT&TAG.

2024-09-25 | E-MTAB-14090 | biostudies-arrayexpress

CUT&TAG of OE19 cell line

Project description:To map the chromatin binding sites for various factors in OE19 cells, we performed CUT&TAG.

2023-02-24 | E-MTAB-11356 | biostudies-arrayexpress

Z-DNA underlies the target choice of Aire by facilitating promoter poising [CUT&Tag]

Project description:We profiled Pol II-pS5 binding in medullary thymic epithelial cells using CUT&Tag to investigate the logic of Aire's target choices

2024-03-11 | GSE224555 | GEO

Role of Setd2 in regulatory T cells [Pol II CUT&Tag]

Project description:To determine the molecular regulation of Tregs by Setd2, spleen Tregs (CD3+CD4+Foxp3-YFP+) from Setd2-deficient and control Tregs were subjected to polII CUT&Tag analysis

2022-10-14 | GSE182839 | GEO

HIF2A(EPAS1) Genome-Wide Localization in MRC5 Human Fibroblasts by Cut & Tag

Project description:HIF2A(EPAS1) geenome_wide localization by Cut & Tag in MRC5 human fibroblasts ectopically expressing HIF2A in the pBabe vector, and in control MRC5 human fibroblasts containing only the pBabe vector. 2 biological replicates were done for the MRC5-HIF2A cells. Cut & Tag with an anti-H3-K27me3 Ab was done as a positive control for the Cut & Tag experiments.

2025-01-17 | E-MTAB-13732 | biostudies-arrayexpress

Bulk Cut&run and Cut&tag

Project description:Bulk Cut&run and Cut&tag

| PRJNA686390 | ENA