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Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

ABSTRACT: Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

PROVIDER: PRJNA1206966 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31899600_1.fastq.gz	Fastqsanger.gz
	SRR31899600_2.fastq.gz	Fastqsanger.gz
	SRR31899601_1.fastq.gz	Fastqsanger.gz
	SRR31899601_2.fastq.gz	Fastqsanger.gz
	SRR31899602_1.fastq.gz	Fastqsanger.gz

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Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

Project description:Anillin (ANLN), a mitotic protein that regulates contractile ring assembly, has been reported as an oncoprotein. However, the function of ANLN in cancer cells, especially in the nucleus, has not been fully understood. Here, we report a previously unknown role of nuclear ANLN in gene transcriptional regulation. We found that nuclear ANLN directly interacts with the RNA polymerase II (Pol II) large subunit. ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and cell differentiation. Our results reveal the function of nuclear ANLN in transcriptional regulation, providing a theoretical basis for ESCC treatment.

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Project description:Anillin (ANLN), a mitotic protein that regulates contractile ring assembly, has been reported as an oncoprotein. However, the function of ANLN in cancer cells, especially in the nucleus, has not been fully understood. Here, we report a role of nuclear ANLN in gene transcriptional regulation. We find that nuclear ANLN directly interacts with the RNA polymerase II (Pol II) large subunit to form transcriptional condensates. ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and cell differentiation. THZ1, a super-enhancer inhibitor, specifically inhibits ANLN-Pol II clustering, target gene expression and esophageal squamous cell carcinoma (ESCC) cell proliferation. Our results reveal the function of nuclear ANLN in transcriptional regulation, providing a theoretical basis for ESCC treatment.

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