Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA120755 | ENA |
REPOSITORIES: ENA
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Project description:Human mesenchymal stem cells (hMSC) have an extensive potential for clinical applications in cell therapy. However, very little is known of the specific molecular regulatory mechanisms that control the therapeutical properties of these cells. We aimed to identify microRNAs (miRNAs) that could be involved in controlling the transition between the self-renewing (undifferentiated) and the reparative (differentiated) phenotypes of hMSCs. MicroRNA microarrays were used to identify miRNAs that are upregulated in undifferentiated hMSCs. For that, we compared the miRNA expression profiles of undifferentiated bone marrow-derived hMSCs with the same primary cell lines after 9 days of in vitro adipogenic or osteogenic induction. We also compared the miRNA expression profiles of undifferentiated hMSCs with skin fibroblasts (a mesenchymal cell lineage with a more restricted differentiation potential). These experiments allowed us to identify miR-335 as the only miRNA downregulated upon MSC differentiation as well as in MSCs in comparison with skin fibroblasts. Gene expression microarrays were used to identify genes that are downregulated in hMSCs overexpressing miR-335. We compared the miRNA expression profiles of hMSCs transduced with a lentiviral vector encoding miR-335 with MSCs transduced with a control lentiviral vector. Our results suggest miR-335 downregulation could be a critical trigger for the initiation of MSCs activities involved in tissue repair and remodelling, including cell proliferation, migration and differentiation.
2010-03-01 | GSE19232 | GEO
Project description:miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells
| PRJNA203222 | ENA
Project description:Human mesenchymal stem cells (hMSC) have an extensive potential for clinical applications in cell therapy. However, very little is known of the specific molecular regulatory mechanisms that control the therapeutical properties of these cells. We aimed to identify microRNAs (miRNAs) that could be involved in controlling the transition between the self-renewing (undifferentiated) and the reparative (differentiated) phenotypes of hMSCs. MicroRNA microarrays were used to identify miRNAs that are upregulated in undifferentiated hMSCs. For that, we compared the miRNA expression profiles of undifferentiated bone marrow-derived hMSCs with the same primary cell lines after 9 days of in vitro adipogenic or osteogenic induction. We also compared the miRNA expression profiles of undifferentiated hMSCs with skin fibroblasts (a mesenchymal cell lineage with a more restricted differentiation potential). These experiments allowed us to identify miR-335 as the only miRNA downregulated upon MSC differentiation as well as in MSCs in comparison with skin fibroblasts. Gene expression microarrays were used to identify genes that are downregulated in hMSCs overexpressing miR-335. We compared the miRNA expression profiles of hMSCs transduced with a lentiviral vector encoding miR-335 with MSCs transduced with a control lentiviral vector. Our results suggest miR-335 downregulation could be one of the triggers for the initiation of MSCs activities involved in tissue repair and remodelling, including cell migration and differentiation. We compared the miRNA expression profiles of undifferentiated bone marrow-derived hMSCs with the same primary cell lines after 9 days of adipogenic or osteogenic induction, as well as with skin fibroblasts. A total of four independent samples were used for each condition. For the adipogenic/osteogenic vs. undifferentiated MSC comparison, the RNA samples were pooled (two independent samples/pool) before labeling. We also compared the miRNA expression profiles of hMSCs transduced with the lentiviral vector pLV-EmGFP-MIRN335 with MSCs transduced with the control vector pLV-EmGFP-Mock. For the gene expression microarrays, a total of three independent samples were used for each condition.
2010-05-06 | E-GEOD-19232 | biostudies-arrayexpress
Project description:Corneal diseases are a leading contributor of vision impairment and their treatment remains challenging. It’s known that enhancement of the stemness of corneal epithelial stem cells (CECs) plays a pivotal role in effective treatments. In this study, we evaluated the effects of extracellular vesicles from human adipose-derived mesenchymal stem cells (AdMSC-EVs) on CECs. Firstly, colony formation assays showed that the colony-forming efficiency (CFE) of CECs significantly increased in the presence of AdMSC-EVs. Then we demonstrated that AdMSC-EVs accelerated migration of CECs in a scratch assay, whereas the proliferation of CECs was suppressed by AdMSC-EVs in cell proliferation assay. RNA Sequencing (RNA-Seq) analysis of CECs implied that AdMSC-EVs maintained the stemness, as well as improved epithelial-mesenchymal transition (EMT). Furthermore, having identified the six top microRNAs (miRNAs) of AdMSC-EVs, CEC transfection with those miRNA mimics, indicated that miR-25, miR-191, and miR-335 were the most effective molecules for improving stemness and EMT. Taken together, our findings indicated that AdMSC-EVs can enhance stemness and EMT of CECs, and AdMSC-EV effects are exerted by the contained miRNAs.
2025-04-30 | GSE255314 | GEO
Project description:Corneal diseases are a leading contributor of vision impairment and their treatment remains challenging. It’s known that enhancement of the stemness of corneal epithelial stem cells (CECs) plays a pivotal role in effective treatments. In this study, we evaluated the effects of extracellular vesicles from human adipose-derived mesenchymal stem cells (AdMSC-EVs) on CECs. Firstly, colony formation assays showed that the colony-forming efficiency (CFE) of CECs significantly increased in the presence of AdMSC-EVs. Then we demonstrated that AdMSC-EVs accelerated migration of CECs in a scratch assay, whereas the proliferation of CECs was suppressed by AdMSC-EVs in cell proliferation assay. RNA Sequencing (RNA-Seq) analysis of CECs implied that AdMSC-EVs maintained the stemness, as well as improved epithelial-mesenchymal transition (EMT). Furthermore, having identified the six top microRNAs (miRNAs) of AdMSC-EVs, CEC transfection with those miRNA mimics, indicated that miR-25, miR-191, and miR-335 were the most effective molecules for improving stemness and EMT. Taken together, our findings indicated that AdMSC-EVs can enhance stemness and EMT of CECs, and AdMSC-EV effects are exerted by the contained miRNAs.
2025-04-30 | GSE255429 | GEO
Project description:Analysis of human mesenchymal stem cells (MSC) from bone marrow and the Wharton's jelly of the umbilical cord after manupulating miR-146a-5p expression. miR-146a-5p is involved in controlling the proliferation and migration of MSCs. Results provide miR-146a-5p-regulating genes in MSCs.
2013-09-05 | GSE44186 | GEO
Project description:Recent development of integrative therapy against melanoma combines surgery, radiotherapy, targeted therapy, and immunotherapy; however, the clinical outcomes of advanced stage and recurrent melanoma are poor. As a skin cancer, melanoma is generally resistant to radiotherapy. Hence, there is an urgent need for evaluation of the mechanisms of radioresistance. The present study identified miR-335 as one of the differential expression of miRNAs in recurrent melanoma biopsies post-radiotherapy. The expression of miR-335 declined in melanoma tissues compared to the adjacent tissues. Moreover, miR-335 expression correlated with advanced stages of melanoma negatively. Consistent with the prediction of STARBASE and miRDB database, miR-335 targeted ROCK1 via binding with 3’-UTR of ROCK1 directly, resulting in attenuation of proliferation, migration and radioresistance of melanoma cells. The authors validated that overexpression of miR-335 enhanced X-ray-induced tumor regression by B16 mouse models. Briefly, the present findings gained insights into miR-335/ROCK1-mediated radiosensitivity and provided a promising therapeutic strategy for improving radiotherapy against melanoma. miRNAs profiling by array
2020-02-13 | GSE143777 | GEO
Project description:Analysis of human mesenchymal stem cells (MSC) from bone marrow and the Wharton's jelly of the umbilical cord after manupulating miR-146a-5p expression. miR-146a-5p is involved in controlling the proliferation and migration of MSCs. Results provide miR-146a-5p-regulating genes in MSCs. In this study, BM-MSCs transduced with miR-146a-5p expression vector or pCDH-CMV-MCS-EF1-copGFP vector only, as well as two WJ-MSCs transfected with short interfering RNAs targeting miR-146a or a GFP control.
2013-09-05 | E-GEOD-44186 | biostudies-arrayexpress
Project description:Human Mesenchymal Stem Cells (hMSC) Differentiation Gene Expression
| PRJNA905479 | ENA
Project description:We developed a strategy to generate cardiac progenitor cells from human induced pluripotent stem cells using a novel small molecule.miRNA-sequencing results showed different miRNA expression profile among undifferentiated human induced pluripotent stem cells(hiPSCs), DMSO and ISX-9 treated hiPSCs.In comparsion with DMSO treated cells, ISX-9 upregulated several myogenic miRNAs and cardiac hypertrophy related-miRNAs including miR-335, miR-21, miR-30c,miRNA-181a and miR-214.
2017-12-30 | GSE95390 | GEO