Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA120869 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of human preadipocytes comparing preadipocytes cultured in control media vs co-culture with PBMC's after 3 days. Goal was to elucidate novel expression patterns in preadipocytes during exposure to human immune cells. 2-condition experiment, Preadipocytes+Media vs Preadipocytes+PBMC. Biological replicates: 4 experimental replicates.
2010-12-03 | E-GEOD-19324 | biostudies-arrayexpress
Project description:Transcriptional profiling of human preadipocytes comparing preadipocytes cultured in control media vs co-culture with PBMC's after 3 days. Goal was to elucidate novel expression patterns in preadipocytes during exposure to human immune cells.
2010-12-03 | GSE19324 | GEO
Project description:Transcription profiling of human primary osteoblasts to study the effect of direct or indirect co-culture with human umbilical vein endothelial cells
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Project description:Full title: Expression data from human primary subcutaneous preadipocytes treated with glucocorticoids prior to the initiation of differentiation. Preadipocytes are continuously exposed to glucocorticoids in situ due to both steroid present in the circulatory system as well as adipose tissue specific 11βHSD1 activity. While the effects of glucocorticoids during differentiation are well studied, the effect of exposure of preadipocytes to glucocorticoids prior to differentiation is unknown. We therefore treated confluent human primary preadipocytes drived from subcutaneous adipose tissue with the synthetic glucocorticoid dexamethasone for 48 hours prior to the initiation of differentiation and assessed what effect this had on their subsequent potential to differentiate. We found that pretreatment with glucocorticoids had a priming effect and resulted in increased differentiation of these preadipocytes. Furthermore, this treatment was additive to the effects of glucocorticoids during the initial phase of adipogenesis. Microarray analysis performed subsequent to the pretreatment with glucocorticoids (at the time point at which preadipocytes would have been induced to differentiate) identified glucocorticoid-responsive, candidate genes whose altered expression could mediate these effects. keywords: glucocorticoids, glucocorticoid receptor, preadipocytes, adipogenesis, human primary preadipocytes, subcutaneous, adipose tissue
2009-12-10 | GSE19393 | GEO
Project description:Full title: Expression data from human primary subcutaneous preadipocytes treated with glucocorticoids prior to the initiation of differentiation. Preadipocytes are continuously exposed to glucocorticoids in situ due to both steroid present in the circulatory system as well as adipose tissue specific 11βHSD1 activity. While the effects of glucocorticoids during differentiation are well studied, the effect of exposure of preadipocytes to glucocorticoids prior to differentiation is unknown. We therefore treated confluent human primary preadipocytes drived from subcutaneous adipose tissue with the synthetic glucocorticoid dexamethasone for 48 hours prior to the initiation of differentiation and assessed what effect this had on their subsequent potential to differentiate. We found that pretreatment with glucocorticoids had a priming effect and resulted in increased differentiation of these preadipocytes. Furthermore, this treatment was additive to the effects of glucocorticoids during the initial phase of adipogenesis. Microarray analysis performed subsequent to the pretreatment with glucocorticoids (at the time point at which preadipocytes would have been induced to differentiate) identified glucocorticoid-responsive, candidate genes whose altered expression could mediate these effects. keywords: glucocorticoids, glucocorticoid receptor, preadipocytes, adipogenesis, human primary preadipocytes, subcutaneous, adipose tissue Experiment Overall Design: Human subcutaneous primary preadipocytes were purchased from Zen-Bio Inc. Preadipocytes from 5 female donors (average BMI 22.5±0.2kg/m2) were pooled prior to the initial seeding in T75 flasks. Cells were maintained at 5% CO2 in DMEM with 1.0g/L glucose, 20% calf serum, 100U/ml penicillin, 100mg/ml streptomycin and 50U/ml nystatin. Cells were expanded once prior to seeding in Nunc-brand 12-well dishes. Upon reaching confluence (24 h post-splitting), preadipocytes were stimulated with vehicle or 1uM dex for 48 hours in growth media containing 3% calf serum. Microarray analysis was performed on duplicate samples.
2009-12-19 | E-GEOD-19393 | biostudies-arrayexpress
Project description:Microarray analysis comparing gene expression profiles of primary cultured preadipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects (a subset of the subjects from the adipocyte genechip project, GSE2508). Primary cultured abdominal subcutaneous preadipocytes from 14 lean (7 Males / 7 Females) and 14 obese (7M/7F) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U133A and B.
2007-08-25 | E-GEOD-2510 | biostudies-arrayexpress
Project description:RNAseq of human bone marrow mesenchymal stem cells in co-culture with endothelial cells
2025-01-23 | E-MTAB-6849 | ExpressionAtlas
Project description:Human subcutaneous preadipocytes RNAseq
| PRJNA736742 | ENA
Project description:Microarray analysis comparing gene expression profiles of primary cultured preadipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects (a subset of the subjects from the adipocyte genechip project, GSE2508). Primary cultured abdominal subcutaneous preadipocytes from 14 lean (7 Males / 7 Females) and 14 obese (7M/7F) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U133A and B. Keywords = Obesity Keywords = inflammation Keywords = microarray Keywords = gene expression Keywords = preadipocyte Keywords = stromal-vascular cells Keywords = adipose tissue Keywords: other
2005-07-28 | GSE2510 | GEO
Project description:Human primary subcutaneous preadipocytes treated with glucocorticoids prior to the initiation of differentiation
| PRJNA121753 | ENA