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Opto-CLIP reveals dynamic FMRP regulation of mRNAs upon CA1 neuronal activation [RiboTag]

ABSTRACT: Opto-CLIP reveals dynamic FMRP regulation of mRNAs upon CA1 neuronal activation [RiboTag]

PROVIDER: PRJNA1209014 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31954651_1.fastq.gz	Fastqsanger.gz
	SRR31954651_2.fastq.gz	Fastqsanger.gz
	SRR31954652_1.fastq.gz	Fastqsanger.gz
	SRR31954652_2.fastq.gz	Fastqsanger.gz
	SRR31954653_1.fastq.gz	Fastqsanger.gz

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Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation [RiboTag]

Project description:A central question in protein synthesis–dependent synaptic plasticity is how translation of neuronal mRNAs is dynamically regulated in time and space. Here, we develop Opto-CLIP, integrating optogenetic stimulation with cell-type-specific crosslinking immunoprecipitation (CLIP) of FMRP in CA1 neurons to define activity-dependent RNA regulation. Following patterned neuronal activation, we identify dynamic changes in FMRP-mediated translational control at minute-scale resolution. Within five minutes, FMRP is released from synaptic transcripts, accompanied by increased ribosome association. By 30 minutes, FMRP dissociates from transcripts encoding nuclear and RNA regulatory factors, also accompanied by increased ribosome association. At this stage, despite a marked reduction in protein levels, FMRP selectively reassociates with synaptic transcripts, coincident with reduced ribosome association. These coordinated shifts define temporally structured waves of translational control linking synaptic and nuclear programs. Our findings reveal a biphasic program of FMRP-mediated translational control that couples local synaptic responses to delayed nuclear regulation, providing a mechanistic framework linking activity-dependent RNA regulation, its disruption in Fragile X syndrome, and homeostatic plasticity.

2025-01-15 | GSE286381 | GEO

Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation [FMRP-CLIP]

2025-01-15 | GSE286379 | GEO

Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation [FMRP-CLIP]

Project description:Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation [FMRP-CLIP]

| PRJNA1209012 | ENA

Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation

Project description:Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation

| PRJNA1452928 | ENA

The Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase in neurons

Project description:In this study, we sought to identify the mRNAs associated to FMRP protein in mouse cortical neuron using a cross linking immunoprecipitation and microarray (CLIP-microarray). The mRNAs crosslinked at 254 nm to FMRP in mouse cortical neurons cultured 8 days in vitro (8 DIV) were immunoprecipitated with H120 anti-FMRP (Santa Cruz) and reverse transcribed to labeled cDNAs with Ovation Pico WTA system V2 (Nugen) and hybridized on Mouse Gene 1.0ST (Affymetrix) We analyzed total RNA (Input) and FMRP-CLIPed RNA (Clip) from 10 primary cortical neuron mouse cultures (5 Wt Input, 5 FMR1-KO Input, 5 Wt Clip, 5 FMR1-KO Clip). Array data was processed by Affymetrix Exon Array Computational Tool. No technical replicates were performed.

2016-06-01 | E-GEOD-51649 | biostudies-arrayexpress

Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation

Project description:This SuperSeries is composed of the SubSeries listed below.

2026-04-13 | GSE327880 | GEO

Role of FMRP in neurogenesis

Project description:Fragile X syndrome (FXS) is a rare disease but is the most common form of inherited intellectual disability and a leading cause of autism. FXS is due to the absence of the Fragile X Mental Retardation Protein (FMRP), an RNA-binding protein mainly involved in translational control. Even if this molecular defect is known, no specific therapy is available for FXS. The first alteration observed in the brain of FXS patients and of Fmr1 KO mice, model for FXS, is represented by an abnormal dendritic morphology that is associated with an altered synaptic plasticity. These findings led to numerous studies focused on mature neurons. However, recently, an increasing body of evidence is pointing out the importance of FMRP in the early steps of brain development. Thus, with the purpose to decipher the earliest molecular events leading to FXS we developed a stem-cell-based disease model by knocking-down the expression of Fmr1 in mouse embryonic stem (ES) cells. To gain insights in the pathways that are affected when FMRP is repressed, we performed a gene expression profile analysis comparing total RNA from shFmr1 and shControl ES cells using whole genome mouse microarrays. Transcripts were clustered according to their Gene Ontology classification using the DAVID software. Surprisingly, the most altered functional category was Nervous system development and function, highlighting the role of FMRP also during the earliest steps of neural development. To study the precise role of FMRP in Embryonic stem (ES) cells, we used an RNAi-based loss-of-function strategy by transducing mouse ES cells with a lentivirus expressing GFP and a shRNA targeting the constitutive exon 1 of Fmr1 (shFmr1). A random shRNA (shCT) was used as a control sequence. Transduced cells were sorted by flow cytometry and established as non-clonal cell populations. RNA samples were harvested and profiling experiments were performed in â??dye-balanceâ?? as indicated for each replicate.

2016-07-04 | E-GEOD-71184 | biostudies-arrayexpress

FMRP cTag CLIP from mouse CA1 pyramidal neurons

Project description:Loss of the neuronal RNA binding protein FMRP causes Fragile X Syndrome (FXS), the most common cause of inherited intellectual disability, yet it is unknown which brain regions and cell types within them contribute to disease pathophysiology. We used conditional tagging of FMRP and CLIP (cTag FMRP CLIP) to examine FMRP targets specifically in CA1 hippocampal neurons, a critical cell type for learning and memory known to have altered synaptic function in FXS. Integrating this data with analysis of ribosome-bound transcripts from the same neuronal population revealed CA1-enriched binding of autism-relevant mRNAs, and unexpected CA1-specific regulation of transcripts encoding circadian proteins.

2020-01-07 | GSE127846 | GEO

The Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase in neurons

2016-06-01 | GSE51649 | GEO

Regulatory Discrimination of mRNAs by FMRP Controls Adult Neural Stem Cell Differentiation

Project description:Fragile X syndrome (FXS) is caused by the loss of fragile X mental retardation protein (FMRP), a translation-inhibitor RNA binding protein. The impact of FMRP-deficiency on neural function is widespread, including its regulation of adult neural stem cell (aNSC) differentiation. To assess FMRP activity, we performed ribosome profiling of aNSCs from normal and Fmr1 knockout mice, which revealed diverse gene expression changes at the mRNA and translation levels. Many mitosis and neurogenesis genes were dysregulated primarily at the mRNA level, while numerous synaptic genes were mostly dysregulated at the translation level. Translational “buffering” was also evident, whereby changes in ribosome association with mRNA is compensated by alterations in RNA abundance. Our data revealed that FMRP-regulated neurogenesis is mediated by the transcriptional factor necdin and that FMRP determines mitochondrial mRNA expression and energy homeostasis. Thus, FMRP controls diverse transcriptional and post-transcriptional gene expression programs critical for the adult neural stem cell differentiation.

2018-11-20 | GSE112502 | GEO

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