Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos

ABSTRACT: Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos

PROVIDER: PRJNA1209118 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos [BRB-seq]

Project description:Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos [BRB-seq]

| PRJNA1208519 | ENA

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos [scRNA-seq]

Project description:Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos [scRNA-seq]

| PRJNA1208511 | ENA

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos [BRB-seq]

Project description:Understanding the interplay between cell fate specification and morphogenetic changes remains a central challenge in developmental biology. Gastruloids, self-organizing pseudo-embryos that mimic post-implantation mammalian development, provide a powerful platform to address this question. Here, we show that physical parameters, particularly system size, critically influence the timing and outcomes of morphogenetic processes. Larger gastruloids exhibit delayed symmetry breaking, increased multipolarity, and prolonged axial elongation, with morphogenesis driven by system size. Despite these variations, transcriptional programs and cell fate composition remain remarkably stable across a broad size range. Notably, extreme sizes show distinct transcriptional modules and clear shifts in gene expression patterns. Intriguingly, size perturbation experiments rescued the morphogenetic and pattern phenotypes observed in extreme sizes, demonstrating the remarkable adaptability of gastruloids to their effective system size. These findings establish gastruloids as versatile models for studying spatiotemporal dynamics in mammalian embryogenesis and reveal how physical constraints decouple transcriptional from morphogenetic programs.

2025-05-07 | GSE286276 | GEO

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos [scRNA-seq]

2025-05-07 | GSE286273 | GEO

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudo-embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-05-07 | GSE286428 | GEO

Resolving single-cell gene expression by pseudo-temporal integration of transcriptomic and proteomic datasets.

Project description:One of the greatest prospects of multimodal single-cell analysis is the ability to integrate single-cell transcriptomic and proteomic datasets to compare transcription and translation profiles to investigate regulation between phenotypes. However, the integration of these complex datasets remains challenging. In this study, we demonstrate the use of pseudo-temporal cell orders as a mutual axis for integrating of scRNA-Seq and scp-MS data. We collected temporal samples of HEK293F cells undergoing hypoxia to isolate the variance of hypoxia from the tightly coupled variance of cell cycle. Markers for hypoxia were then used to construct pseudo-temporal orders in each dataset which were used to reveal transcription and translation dynamics of attenuated respiration in single cells. This approach allowed us to integrate single-cell transcriptomics and proteomics methods to understand the regulatory mechanisms governing biological phenotypes.

2024-12-01 | GSE273172 | GEO

Pseudo-nitzschia multiseries

Project description:Pseudo-nitzschia multiseries overview

| PRJNA287468 | ENA

Pseudo-nitzschia multistriata

Project description:Pseudo-nitzschia multistriata overview

| PRJNA84105 | ENA

Pseudo-nitzschia multistriata

Project description:Pseudo-Nitzschia multistriata Project

| PRJNA80045 | ENA

Pseudo-nitzschia multistriata

Project description:Pseudo-nitzschia multistriata transcriptome