Project description:Single cell RNA-seq samples from 3 species across a differentiation from induced pluripotent stem cells to neural progenitor cells were generated to study gene expression evolution. Briefly, previously generated urinary stem cell derived iPSCs of 3 human (Homo sapiens) individuals (3 clones), 1 gorilla (Gorilla gorilla) individual (2 clones) and fibroblast derived cynomolgus macaque (Macaca fascicularis) iPSCs of 2 individuals (4 clones) (Geuder et al. 2021) were differentiated to neural progenitor cells via dual-SMAD inhibition as three-dimensional aggregation culture (Chambers et al. 2009; Ohnuki et al. 2014). Single cell RNA-seq libraries from differentiation days 0, 1, 3, 5, 7, 9 were generated using mcSCRB-seq protocol (Bagnoli et al. 2018).
Project description:In vitro challenge of primary hepatocytes, with the Infectious pancreatic necrosis (IPN) virus. Cells from were infected at MOI 0.1 with two different isolates of IPNV: V1244 and Vir410/2018. V1244 represents classical IPNV that is affected by the IPN-QTL, while Vir410/2018 is the QTL-insensitive or recent isolate, rIPNV in short.
