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Gene expression in different root types in pearl millet (Pennisetum glaucum (L.) R. Br)

ABSTRACT: Gene expression in different root types in pearl millet (Pennisetum glaucum (L.) R. Br)

PROVIDER: PRJNA1210518 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31994035_1.fastq.gz	Fastqsanger.gz
	SRR31994035_2.fastq.gz	Fastqsanger.gz
	SRR31994036_1.fastq.gz	Fastqsanger.gz
	SRR31994036_2.fastq.gz	Fastqsanger.gz
	SRR31994037_1.fastq.gz	Fastqsanger.gz

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Gene expression in different root types in pearl millet (Pennisetum glaucum (L.) R. Br)

Project description:Pearl millet (Pennisetum glaucum) is a cereal crop well-adapted to arid and high-temperature environments. Its root system exhibits a high degree of complexity, comprising distinct root types with specific anatomical and functional characteristics. These include the primary root, which is established during embryogenesis; crown roots, which develop at the base of the stem post-embryonically; and lateral roots, which emerge from both the primary and crown roots. In this study, we conducted a comparative analysis of gene expression profiles across these distinct root types to better understand their functional specialization.

2025-01-29 | GSE286898 | GEO

Comparison of the root tip transcriptome of 2 pearl millet lines with contrasted root soil aggregation

Project description:We analyzed global gene expression in the crown tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 220) root soil aggregation using RNAseq. The obtective was to identify genes potentially associated with changes in rhizosheath formation.

2021-10-09 | GSE185425 | GEO

Comparison of the root tip transcriptome of 2 pearl millet lines with contrasted primary root growth

Project description:We analysed global gene expression in the primary root tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 337) primary root growth using RNAseq. The objective was to identify genes potentially associated with changes in root growth.

2021-10-10 | GSE185517 | GEO

Proteomic analysis of Pennisetum glaucum under drought stress

Project description:Pearl millet [Pennisetum glaucum (L.) R.Br] is the fifth most important cereal crop next to rice, wheat, maize, and sorghum. It is cultivated especially by small holder farmers in arid and semi-arid regions because of its drought resistance. However, the molecular mechanisms during drought stress in Pennisetum remain elusive. In the present study we have used a shotgun proteomics approach (GEL-LC-Orbitrap-MS) for identification and quantification of proteins from different tissues (root, seed and leaf) under drought and control conditions. Plants were grown in a tube system to survey root growth under drought stress. The water content was measured in the upper and the lower part of the tube using soil moisture sensors. Under drought stress root elongation was observed. Measurement of stomatal conductance showed a clear response to drought stress. For proteomics measurements root, leaf and seed tissues were harvested. In total 2281 proteins were identified, 1095 in root, 1299 in seed, and 1208 in leaf in both stress and control conditions.

2023-08-23 | PXD002923 | Pride

Pearl millet seedlings infected with rust, or treated with MeJA or SA

Project description:This SuperSeries is composed of the following subset Series: GSE13478: Pearl millet seedlings treated with methyl jasmonate (MeJA) GSE13479: Pearl millet seedlings infected with rust (Puccinia substriata) GSE13480: Pearl millet seedlings treated with salicylic acid (SA) Refer to individual Series

2010-05-14 | E-GEOD-13481 | biostudies-arrayexpress

Towards Defining Heterotic Gene Pools in Pearl Millet [Pennisetum glaucum (L.) R. Br.].

Project description:Pearl millet is a climate resilient crop and one of the most widely grown millets worldwide. Heterotic hybrid development is one of the principal breeding objectives in pearl millet. In a maiden attempt to identify heterotic groups for grain yield, a total of 343 hybrid parental [maintainer (B-) and restorer (R-)] lines were genotyped with 88 polymorphic SSR markers. The SSRs generated a total of 532 alleles with a mean value of 6.05 alleles per locus, mean gene diversity of 0.55, and an average PIC of 0.50. Out of 532 alleles, 443 (83.27%) alleles were contributed by B-lines with a mean of 5.03 alleles per locus. R-lines contributed 476 alleles (89.47%) with a mean of 5.41, while 441 (82.89%) alleles were shared commonly between B- and R-lines. The gene diversity was higher among R-lines (0.55) compared to B-lines (0.49). The unweighted neighbor-joining tree based on simple matching dissimilarity matrix obtained from SSR data clearly differentiated B- lines into 10 sub-clusters (B1 through B10), and R- lines into 11 sub-clusters (R1 through R11). A total of 99 hybrids (generated by crossing representative 9 B- and 11 R- lines) along with checks were evaluated in the hybrid trial. The 20 parents were evaluated in the line trial. Both the trials were evaluated in three environments. Based on per se performance, high sca effects and standard heterosis, F1s generated from crosses between representatives of groups B10R5, B3R5, B3R6, B4UD, B5R11, B2R4, and B9R9 had high specific combining ability for grain yield compared to rest of the crosses. These groups may represent putative heterotic gene pools in pearl millet.

| S-EPMC5841052 | biostudies-literature

A Spatiotemporal Transcriptome Revealed Stalk Development in Pearl Millet

Project description:Pearl millet is a major cereal crop that feeds more than 90 million people worldwide in arid and semi-arid regions. The stalk phenotypes of Poaceous grasses are critical for their productivity and stress tolerance, however, the molecular mechanisms governing stalk development in pearl millet remained to be deciphered. In this study, we spatiotemporally measured 19 transcriptomes for stalk internodes of four different early developmental stages. Data analysis of the transcriptomes defined 4 developmental zones on the stalks and identified 12 specific gene sets with specific expression patterns across the zones. Using weighted gene co-expression network analysis (WGCNA), we found that 2 co-expression modules together with candidate genes were involved in stalk elongation and thickening of pearl millet. Among the elongation-related candidate genes, we established by SELEX that a MYB-family transcription factor PMF7G02448 can bind to the promoters of three cell wall synthases genes (CesAs). In summary, these findings provide insights into stalk development and offer potential targets for future genetic improvement of pearl millet.

2024-09-15 | GSE268902 | GEO

Pearl millet seedlings infected with rust (Puccinia substriata)

Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response

2009-01-01 | GSE13479 | GEO

Pearl millet seedlings treated with salicylic acid (SA)

Project description:Transcriptional profiling of SA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response

2009-01-01 | GSE13480 | GEO

Pearl millet seedlings treated with methyl jasmonate (MeJA)

Project description:Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response

2009-01-01 | GSE13478 | GEO

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