Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA) and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples

PROVIDER: PRJNA1210853 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR32006200_1.fastq.gz	Fastqsanger.gz
	SRR32006200_2.fastq.gz	Fastqsanger.gz
	SRR32006189_1.fastq.gz	Fastqsanger.gz
	SRR32006189_2.fastq.gz	Fastqsanger.gz
	SRR32006190_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Transcriptomic analysis of PLA-degrading bacteria Brevibacillus brevis grown on polylactic acid

Project description:Polylactic acid (PLA) is a promising biodegradable material used in various fields, such as mulching films and disposable packaging materials. Biological approaches for completely degrading biodegradable polymers can provide environmentally friendly solutions. However, to our knowledge, no studies have performed transcriptome profiling to analyze PLA-degrading genes of PLA-degrading bacteria. Therefore, this study reports for the first time an RNA sequence approach for tracing genes involved in PLA biodegradation in the PLA-degrading bacterium Brevibacillus brevis. In the interpretation results of the differentially expressed genes, the hydrolase genes mhqD and nap and the serine protease gene besA were up-regulated by a fold change of 7.97, 4.89, and 4.09, respectively. This result suggests that hydrolases play a key role in PLA biodegradation by B. brevis. In addition, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that genes implicated in biofilm formation were upregulated. The biodegradation of PLA starts with bacteria attaching to the surface of PLA and forming a biofilm. Therefore, it could be confirmed that the above genes were up-regulated for access to PLA and biodegradation. Our results provide transcriptome-based insights into PLA biodegradation, which pitch a better understanding of microbial biodegradation of plastics.

2025-01-16 | GSE223668 | GEO

Bacteria

Project description:A Novel approach to enhance the marine biodegradability of poly(butylene succinate)

| PRJDB17964 | ENA

Project description:Marine copepod culture as a potential source of bioplastic-degrading microbiome: The case of poly(butylene succinate-co-adipate)

| PRJNA1288420 | ENA

Project description:Characterisation of microbial communities during composting of poly(butylene succinate)-wheat bran composites

| PRJNA1087318 | ENA

Plastics investigated can be mineralized by bacteria naturally occurring within the marine environment

Project description:Biodegradable plastics are one possible solution for reducing plastic waste, yet the mechanisms and organisms involved in their degradation in the aquatic environment remain understudied. In this study, we have enriched a microbial community from North Sea water and sediment, capable of growing on the polyester poly(butylene succinate). This culture was grown on two other biodegradable polyesters, polycaprolactone and ecovio® FT (a PBAT-based blended biodegradable plastic), and the differences between community structure and activity on these three polymers were determined by metagenomics and metaproteomics. We have seen that the plastic supplied drives the community structure and activity. Setups growing on ecovio® FT were more diverse, yet showed the lowest degradation, while poly(butylene succinate) and polycaprolactone resulted in a less diverse community but much higher degradation efficiencies. The dominating species were Alcanivorax sp., Thalassobius sp., or Pseudomonas sp., depending on the polymer supplied. Furthermore, we have observed that Gammaproteobacteria were more abundant and active within the biofilm and Alphaproteobacteria within the free-living fraction of the enrichments. Two of the three PETase-like enzymes isolated were expressed as tandems (Ple -tan1 &Ple – tan2) and all three were produced by Pseudomonas sp. Of those, Ple-tan1 was most active on all three substrates and also the most thermostable. Overall, we could show that all three plastics investigated can be mineralized by bacteria naturally occurring within the marine environment and characterize some of the enzymes involved in the degradation process.

2023-11-22 | PXD038098 | Pride

Project description:Genomic analysis of Poly(lactic acid) degrading Geobacillus sp (strain EC-3)

| PRJNA721072 | ENA

Polyesterase discovery in Pseudomonas pseudoalcaligenes secretome

Project description:By screening the secretomes of polymer induced Pseudomonas pseudoalcaligenes we identify a new enzyme PpEst that can degrade the co-aliphatic-aromatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT). The discovered enzyme has predicted arylesterase activity and is induced by PBAT added to the growth medium

2016-12-13 | PXD004014 | Pride

Massilia strain:N-3

Project description:PLA-degrading bacterium Massilia sp. N-3

| PRJDB14675 | ENA

Project description:Genomic analysis of Poly(lactic acid) degrading Pseudomonas aeruginosa (strain S3) and Sphingobacterium sp. (strain S2)

| PRJNA474620 | ENA

Project description:Metagenomes of North Sea enrichments degrading PBS

| PRJNA887682 | ENA