Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: TLA reads of Lifeact.mScarlet-I vector intergation site in mouse model

PROVIDER: PRJNA1214545 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR32089531_1.fastq.gz	Fastqsanger.gz
	SRR32089531_2.fastq.gz	Fastqsanger.gz
	SRR32089532_1.fastq.gz	Fastqsanger.gz
	SRR32089532_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

TLA: Targeted Locus Amplification interaction libraries from TLA viewpoints in the GFP construct from GFP(CAG) cells.

Project description:We performed Targeted Locus Amplification (TLA) to map the insertion site of a stably integrated, hemizygous ectopic locus that contains CAG repeats (15 or 270 repeats) in clonal populations of HEK293-derived cells (GFP(CAG)15 or GFP(CAG)270).

2020-04-07 | GSE148183 | GEO

The longitudinal kinetics of AAV5 vector integration profiles and evaluation of clonal expansion in mice

Project description:Recombinant adeno-associated virus (AAV)-based vectors are used clinically for gene transfer and persist as extrachromosomal episomes. A small fraction of vector genomes can integrate into the host genome, but the theoretical risk of tumorigenesis may depend on vector regulatory features. A mouse model was used to investigate long-term kinetics and integration profiles of an AAV serotype 5 (AAV5) vector that mimics key features of valoctocogene roxaparvovec (AAV5-hFVIII-SQ), a gene therapy for severe hemophilia A. The majority (95%) of vector genome reads identified by target enrichment sequencing were derived from episomes, and mean integration frequency was 2.70 (standard deviation, 1.24) integrations per 1000 cells. Longitudinal integration analysis suggested AAV5 vector integrations occur primarily within 1 week, at low frequency, and their abundance was stable over time. Integration profiles were polyclonal, and only 5.46% of integrations had a common integration site order ≥5, suggesting random distributions when looking at a 50-kb genomic window. No integrations were associated with clonal expansion. Integrations were enriched near transcription start sites of genes highly expressed in the liver (P = 1x10−4) and less enriched for genes with low or no liver expression. We found no evidence of tumorigenesis or fibrosis caused by the vector integrations.

2024-02-01 | GSE254503 | GEO

Genome wide transcriptome analysis (RNA-seq) of adult hippocampal neural stem cells in vitro a.k.a. HCN cells in quiescent and proliferative conditions in non-electroporated or electroporated with control empty vector or REST knock-down shRNA vector

Project description:We report the high-throughput transcriptome profiling of induced quiescent (iQNP) and proliferative (TAP) conditions in mammalian adult hippocampal stem cells in culture (HCN cells). By obtaining 145 to 78 million pair-end reads of sequence from isolated RNA from HCN cells in iQNP and TAP conditions. Thereafter, we intersected this gene expression data with REST bound ChIP-seq peaks within +/-10kb of transcription start site of genes also from HCN cells in iQNP and TAP conditions. We find that with +/-10kb of transcription start site of genes, REST efficiently binds neuronal genes and represses them in HCN cells in both iQNP and TAP conditions. Moreover, only in the iQNP REST also binds non-neuronal genes like DNA replication genes. We also report the high-throughput transcriptome profiling of iQNP and TAP condition HCN cells electroporated with a control empty vector or REST knock-down shRNA vector. By obtaining 145 to 78 million pair-end reads of sequence from isolated RNA from HCN cells in electroporated control empty vector or REST knock-down vector in iQNP and TAP conditions. In REST knockdown in iQNP and TAP conditions we found that predominantly non-neuronal genes and neuronal genes were derepressed. This study reveals that in addition to its well known function as a neuronal repressor in non-neuronal tissue, REST can also play other diverse roles in non-neuronal tissues.

2016-11-09 | GSE70696 | GEO

mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy

Project description:Large genes including several CRISPR-Cas modules, such as gene activators (CRISPRa), require dual adeno-associated viral (AAV) vectors for efficient in vivo delivery and expression. Current dual AAV vector approaches have important limitations, e.g., low reconstitution efficiency, production of alien proteins, or low flexibility in split site selection. Here, we present a dual AAV vector technology based on reconstitution via mRNA trans-splicing (REVeRT). REVeRT is flexible in split site selection and can efficiently reconstitute different split genes in numerous in vitro models, in human organoids and in vivo. Furthermore, REVeRT can functionally reconstitute a CRISPRa module targeting genes in various mouse tissues and organs in single or multiplexed approaches upon different routes of administration. Finally, supplementation of ABCA4 (6.8 kb) via REVeRT improves retinal degeneration and function in a mouse model of inherited blindness. Due to its flexibility and efficiency REVeRT harbors great potential for basic research and clinical applications.

2023-03-18 | GSE198863 | GEO

RNA sequencing data after simultaneous transactivation of M-opsin and knockout of rhodopsin upon subretinal injection of mice with the REVeRT dual AAV system

2023-09-06 | GSE198893 | GEO

Single cell sequencing datasets of tibial nerves from diabetic neuropathy (DPN) patients and traumatic limb amputation (TLA) patients.

Project description:To understand the cellular diversity and alterations in the pathogenesis of diabetic neuropathy (DPN), weenrolled 4 DPN patients, 3 traumatic limb amputation (TLA) patients at the Department of Orthopedic Surgery of Shanghai Sixth People’s Hospital, Shanghai, China.

2024-12-18 | GSE266026 | GEO

Whole Exome Sequencing of cohorts of Mutant Braf mouse model melanoma DNA and germline DNA.

Project description:Whole Exome Sequencing of cohorts of Mutant Braf mouse model melanoma DNA and germline DNA. The cohorts are (1) Mutant Braf mouse model melanomas, (2) Mutant Braf mouse model melanomas from UVR exposed mice and (3) Mutant Braf mouse model melanomas from UVR exposed, sunscreen protected mice.

| EGAS00001000729 | EGA

Generation of a new mouse model of glaucoma characterized by reduced expression of the AP-2 and AP-2

Project description:This study reports on the generation of a transgenic mouse which is characterized by early-onset angle-closure glaucoma. The phenotype is due to the transgene insertion site which is proximal to the genes encoding AP-2 and AP-2, two proteins present in the retina and corneal epithelium. This leads to a reduction in AP-2 levels, which is likely to are responsible for the glaucomatous phenotype. Here we provide paired-end raw DNA-Seq reads of the transgenic mouse genome.

2017-03-10 | E-MTAB-5502 | biostudies-arrayexpress

Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))

Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation Four comparisons were made, all with log phase cultures and no ATc added. Darr Site(+) with an empty vector compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing WT Arr compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing ArrH18A compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing Arr D83A compared to wild-type M. smegmatis Site(+). 3 biological replicates of each strain for each experiment.

2011-07-14 | E-GEOD-30635 | biostudies-arrayexpress

Project description:TLA induces mucus via IL13

| PRJNA735223 | ENA