Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - Timelapse sequencing data of Transcription Factor induced Microglia Like cells

ABSTRACT: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - Timelapse sequencing data of Transcription Factor induced Microglia Like cells

PROVIDER: PRJNA1214810 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR32095300_1.fastq.gz	Fastqsanger.gz
	SRR32095300_2.fastq.gz	Fastqsanger.gz
	SRR32095301_1.fastq.gz	Fastqsanger.gz
	SRR32095301_2.fastq.gz	Fastqsanger.gz
	SRR32095302_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - Timelapse sequencing data of Transcription Factor induced Microglia Like cells

Project description:Differentiation of induced pluripotent stem cells (iPSCs) into specialized cell types is essential for uncovering cell-type specific molecular mechanisms and interrogating cellular function. Transcription factor (TF) screens have enabled efficient production of a few cell types; however, engineering cell types that require complex TF combinations remains challenging. Here, we report an iterative, high-throughput single-cell TF screening method that enables identification of TF combinations for specialized cell differentiation, which we validated by differentiating human microglia-like cells. We found that the expression of six TFs, SPI1, CEBPA, FLI1, MEF2C, CEBPB, and IRF8, is sufficient to differentiate human iPSC into cells with transcriptional and functional similarity to primary human microglia within 4 days.

2025-03-23 | GSE287851 | GEO

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - TFiMGLs are responsive to disease-relevant stimuli

2025-03-23 | GSE287853 | GEO

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - First round of pooled screening identified an initial set of TFs for inducing microglia gene expression.

2025-03-23 | GSE287847 | GEO

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - Second round of pooled screening identified an initial set of TFs for inducing microglia gene expression.

2025-03-23 | GSE287850 | GEO

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC -Single cell transcriptome timecourse analysis of TFiMGLs after SCF-MCI (SPI1, FLI1, CEBPA, CEBPB, IRF8, and Mef2C) induction.

Project description:Transcription factor (TF) screens have enabled efficient production of a few cell types; however, engineering cell types that require complex TF combinations remains challenging. Here, we report an iterative, high-throughput single-cell TF screening method that enables identification of TF combinations for specialized cell differentiation, which we validated by differentiating human microglia-like cells. We found that the expression of six TFs, SPI1, CEBPA, FLI1, MEF2C, CEBPB, and IRF8, is sufficient to differentiate human iPSC into cells with transcriptional and functional similarity to primary human microglia within 4 days.

2025-03-23 | GSE287852 | GEO

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - TFiMGLs are responsive to disease-relevant stimuli

Project description:Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - TFiMGLs are responsive to disease-relevant stimuli

| PRJNA1214809 | ENA

Project description:Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - First round of pooled screening identified an initial set of TFs for inducing microglia gene expression.

| PRJNA1214808 | ENA

Project description:Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - Second round of pooled screening identified an initial set of TFs for inducing microglia gene expression.

| PRJNA1214811 | ENA

Oncogenic transcription factor targets

Project description:Oncogenic transcription factor targets

| PRJNA1251094 | ENA

TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding

Project description:RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.

2018-01-12 | GSE95854 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data